Effects of Dietary Probiotic on Performance, Noxious Gas Emission and Microflora Population on the Cecum in Broiler

복합 생균제 첨가가 육계 생산성, 유해가스 발생량 및 맹장내 균총에 미치는 영향

  • Ko, Y.D. (Division of Applied Life Science, Gyeongsang National University) ;
  • Sin, J.H. (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, S.C. (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Y.M. (Division of Applied Life Science, Gyeongsang National University) ;
  • Park, K.D. (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, J.H. (Korea Applied Microorganism Institute Research)
  • 고영두 (경상대학교 응용생명과학부) ;
  • 신재형 (경상대학교 응용생명과학부) ;
  • 김삼철 (경상대학교 응용생명과학부) ;
  • 김영민 (경상대학교 응용생명과학부) ;
  • 박기동 (경상대학교 응용생명과학부) ;
  • 김재황 (한국응용미생물산업연구소)
  • Published : 2003.08.30


This study was carried out to investigate the effect of probiotics on the performance, nutrients digestibility, noxious gas emission and microflora population on the cecum of broilers. A total of 120 broilers, consisted of 4 treatments X 3 replicates X 10 broilers per replicates, were fed the experimental diets containing 0, 0.1, 0.3 and 0.5% probiotics for 5 weeks. Broilers fed the diets containing 0.1 and 0.3% probiotic had higher (p<0.05) body weight gain and feed conversion than those of the others from the 3rd to 4th week. Broilers fed 0.3% probiotic had higher (p<0.05) body weight gain and feed conversion than those of the other levels from the 5th to 6th week. Broilers fed the diets containing 0.1% and 0.3% probiotic had higher (p<0.05) body weight gain and feed conversion than those of the others from the 2nd to 6th week. Dry matter digestibility was significantly (p<0.05) improved with 0.3% probiotic. Emission of ammonia and sulfate hydrogen gas was significantly (p<0.05) decreased at 6th week. However, there was no (p<0.05) difference at the levels of 0, 0.1, 0.3 and 0.5% at the 4th weeks. There was an increase in the lactobacillus sp, but there was a decrease in the microflora population of coliforms in the cecum of broiler with 0.1% and 0.3% probiotics. These results indicated that the compound probiotics of 0.1${\sim}$0.3% were effective in the body weight gain, feed conversion, nutrients digestibility, noxious gas emission and microflora population on the cecum in broilers.


Probiotic;Broiler;Performance;Microflora population;Noxious gas


  1. AOAC. 1990. Official method analysis, Association of Official Analytical Chemist. Washington, DC, USA.
  2. Buenrostro, J. I. and Kratzer, F. H. 1983. Effects of lactobacillus inoculation and antibiotic feeding of chicks on availability of dietary biotin. Poult. Sci. 62:2022-2029.
  3. Chiang, S. H. and Hsieh, W. M. 1995. Effect of direct-fed microorganisms on broiler growth performance and litter ammonia level. Asian Aus. J. Anim. Sci. 8(2):159-162.
  4. Duncan, D. B. 1955. Multiple range and multiple F test. Biometerics. 11:1.
  5. Edozien, J. C., Udo, U. U., Joung, V. R. and Scrimshaw, N. S. 1970. Effects of high levels of yeast feeding on uric acid metabolism of young men. Nature. London. 228:180.
  6. Goodling, A. C., Cerniglia, G. J. and Hebert, J. A. 1987. Production performance of white leghorn layers fed lactobacillus fermentation products. Poult. Sci. 66:480-486.
  7. Francis, C., Janky D. M., Arafa A. S. and Harms R. H. 1978. Interrelationship of lactobacillus and zinc bacteriocin in diets of trukey poults. Poult. Sci. 57:1687-1689.
  8. Fuller, R. 1977. The impotance of lactobacilli in maintaining normal microbial balance in crop. Br. Poult. Sci. 18:85-94.
  9. Fuller, R. and Cole, C. B. 1989. The scientific basis of the pro-biotics concept. In B. A. Stark and J. M. Wilkinson(Eds). probioticss; Theory and Applications. 1:14.
  10. Jassim, A. H., Hussain, N. A., Muhssin, A. A., Alakb, M. and Al-Haidary, S. Y. 1986. The effect of using single cell protein on productive performance, level of nucleic acids and histopathological changes of broilers. J. Agri. and Water Resources Res. Anim. Production. 5:275-283.
  11. Lilly D. M. and R. H. Stillwell. 1965. Probiotics : growth promoting factors produced by microorganisms. Science. 147:747-748.
  12. Nahashon, S. N., Nakaue, H. S. and Mirosh, L. W. 1994. Phytase activity, phosphors and calcium retention and performance of single comb white leghorn layers fed diets containing two levels of available phosphorus and supplemented with directed microbials. Poult. Sci. 73:1552-1562.
  13. Paker R. B. 1974. Probiotics, the other half of the antibiotic story. Anim. Nutr. Health. 29:4-8.
  14. Piva, G., Belladonna, S., Fusconi, G. and Sicbaldi, F. 1993. Effect of yeast on dairy cow performance, ruminal fermentation, Blood components and Manufacturing properties. J. Dairy Sci. 76:2717.
  15. Pollman, D. S. 1986. Probiotics in pig diets. J. Anim. Nut. 193 -205.
  16. Rose, A. H. 1980. Recent research on industrially important strains of saccharomycess cerevisiae. In: Skinner, F. A., Passmore, S. M. and Daven, R. R. port(ed.) Biology and Activities of Yeast. The Society for Applied Bacteriology Symposium Series. 9:103. Academic press, London.
  17. SAS. 1990. $SAS/STAT^{(R)}$ User’s Guide, version 6.01, 4th ed.; SAS Institute, Inc., Cary, NC, USA
  18. Sedlacek, O. and Rucki, J. 1976. Presence of residue of drugs meat and intestinal caves fed on a milk mixture. VM. Med./Praga. 21:137.
  19. Shannon, D. W. F. and McNab, J. M. 1972. The effect of different dietary levels cattle wastes. Livestock waste management and pollution abatement. ASAE. p. 314-314.
  20. Underdahl, N. R., Torres-Median, A. and Doster, A. R. 1982. Effect of streptococcus faecium C-68 in the control of escherichia coli-induced diarrhea in gnotobiotic pigs. J. Vet. Res. 43:227.
  21. Visek, W. J. 1978. The mode of growth promotion by antibiotics. J. Anim. Sci. 1447-1469.
  22. Waslie, C. I., Calloway, D. H., Margen, S. and Costa, F. 1970. Uric acid levels in men fed algae and yeast as protein sources. J. Food Sci. 35:294-298.
  23. Watkins, B. A., Miller B. F. and Neil D. H. 1982. In vivo effects of Lactobacillus acidophilus against pathogenic Escherichia coli in gnotobiotic chicks. Poult. Sci. 61:1298-1308.
  24. White, F, G, Wenham, G. A., Shatman, A. S., Jones, E. A., Rattray and McDonald, I. 1969. Stomach function in relation to a scour syndrome in the piglet. Br. J. Nutr. 23:847-58.
  25. Wrong, O. M., Edomonds, C. J. and Chadwick, V. S. 1981. Nitrogen compounds. In: The large Intestine: Its role in mammalian nutrition and homeostasis. J & S, New York. 133-211.
  26. Wu, J. F. 1987. The microbiologist’s function in developing action specific microorganisms. In: T. P. Lyons(ed.). Biotechnology in the feed industry. Alletech, Inc. Nicholas ville, Kentucky. 181.
  27. Yeo, J. M. and Kim, K. I. 1997. Effect of feeding diets containing an antibiotic, a probiotic or yucca extract on growth and intestinal urease activity in broiler chicks. Poult. Sci. 76:381-385.
  28. 고용균, 황영환. 1999. Aspergillus oryzae 균주로 배양한 효모배양물의 급여가 부로일러의 육성성적에 미치는 영향. 한국축산학회지. 41 (1):15-22.
  29. 권오석, 김인호, 홍종욱, 한영근, 이상환, 이제만. 2002. 사료내 생균제의 첨가가 육계의 상장, 혈액성상 및 분내 유해가스 함량에 미치는 영향. 가금학회지. 29(1):1-6
  30. 김재황. 김영민, 김삼철, 하홍민, 고영두, 김창현. 2001. 복합 $생균제(Economix^{(R)})$ 첨가가 육계 생산성 및 계사 내 유해가스 감소에 미치는 영향. 동물자원지. 43(3);349-360.
  31. 김춘수, 이남형. 1974. 석유자화 효모의 사료적 가치에 관한 연구(I). 한국축산학회지. 16(2):125-133.
  32. 남궁환, 손익성, 정진성, 백인기. 1986. 생균제와 항생제가 병아리의 성장과 장내 세균총에 미치는 영향. 가금학회지. 13(1):49.
  33. 류경선, 박홍석. 1998. 생균제의 급여가 육계의 생산성과 장내 미생물의 변화에 미치는 영향. 가금학회지. 25(1)31-37.
  34. 박수영, 김상호, 유동조, 이상진, 류경선. 2001. 유산균의 급여가 육계의 성장능력에 미치는 영향. 가금학회지. 28(1):27-40.
  35. 박형룡, 한인규, 허기남. 1994. Yeast culture의 첨가가 육계의 생산성과 장내 yeast colony에 미치는 영향. 한국영양사료학회지. 18(5):346-354.
  36. 이선재, 최선우, 남궁환, 백인기. 2000. 사료의 단백질 수준과 첨가제들이 육계사내 암모니아가스 발생에 미치는 영향. 한국동물자원과학회지. 42(3):299-314.
  37. 한인규, 이상철, 이진희, 이금기, 이정치. 1984. 생균제제의 성장촉진 효과에 관한 연구; I. 브로일러에 대한 lactobacillus sporogenes의 성장촉진 효과와 분변 및 장내 세균총의 변화에 미치는 영향. 한국동물자원과학회지. 23(2):150-157.

Cited by

  1. Effects of Dietary Bacillus subtilis Supplementation on Meat Quality, Growth Performance and Fecal Malodor Gas Emission in Finishing Pigs vol.28, pp.3, 2008,
  2. Effects of Dietary Supplementation of a New Probiotic CS61 Culture on Performance in Broiler Chickens vol.22, pp.3, 2012,