Electrochemical Determination of the Optimum pH of HRP

전기화학적 방법에 의한 HRP의 최적 pH 도출

  • 윤길중 (청주대학교 이공대학 응용과학부)
  • Received : 2003.07.16
  • Accepted : 2003.10.30
  • Published : 2003.12.25

Abstract

A carbon paste electrode was constructed with peroxidase extracted from Horseradish and the variation of the response of the sensor with pH was investigated. Current profiles showed two highest sensitivities at two pH values respectively. In addition, two bands were observed in the electrophoretic expansion. A coincidence of the two experimental results added support to the possibility that the biosensor has two different isozymes. Assuming that current profiles are the sum of two gaussians, we deconvoluted them and determined the optimum pH of peroxidase isozymes.

Acknowledgement

Supported by : 청주대학교

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