Determination of Amitrole in Water Samples by Evaporation and GC/MS

증발법과 GC/MS를 이용한 수질 시료 중의 Amitrole의 분석법 연구

  • Yoon, So Hee (Bioanalysis & Biotransformation Research Center, Korea Institute of Science & Technology) ;
  • Hong, Ji Eun (Bioanalysis & Biotransformation Research Center, Korea Institute of Science & Technology) ;
  • Pyo, Hee Soo (Bioanalysis & Biotransformation Research Center, Korea Institute of Science & Technology) ;
  • Park, Song Ja (Bioanalysis & Biotransformation Research Center, Korea Institute of Science & Technology)
  • 윤소희 (한국과학기술연구원 생체대사연구센터) ;
  • 홍지은 (한국과학기술연구원 생체대사연구센터) ;
  • 표희수 (한국과학기술연구원 생체대사연구센터) ;
  • 박송자 (한국과학기술연구원 생체대사연구센터)
  • Received : 2003.09.25
  • Accepted : 2003.10.16
  • Published : 2003.12.25

Abstract

Amitrole is well-known as a non-selective herbicide and it is able to cause contamination of driking water as well as pollution of ground water and surface water. However, it is difficult to extract from water because it has a high solubility for water whereas a low solubility for general organic solvents. This method is described for the determination of amitrole in water samples by GC/MS. After evaporation of 10 mL water sample by a vacuum evaporator, amitrole was derivatized with isobutyl chloroformate (iso-BCF) on room temperature for 15~20 min. As a result, the sensitivity for GCfMS was improved as N-isobutoxycarbonyl amitrole derivative was formed. The linearity of the calibration curve showed good as 0.997. The recoveries were obtained more than 94.9% and relative standard deviations were less than 2.8% at $1.0{\mu}g/L$, $10.0{\mu}g/L$ and $100.0{\mu}g/L$. The limit of detection showed $0.1{\mu}g/L$ with a signal-to-noise ratio (S/N) of 3.

Acknowledgement

Supported by : 과기부

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