Determination of Volatile Fatty Acids in Aqueous Samples by HS-SPME with In-Fiber Derivatization

Fiber내 유도체화/HS-SPME를 이용한 수용액 시료 중 휘발성 지방산의 분석

  • Ahn, Yun Gyong (Hazardous substance research team, Korea Basic Science Institute) ;
  • Lee, Jee Yeon (Hazardous substance research team, Korea Basic Science Institute) ;
  • Kim, Jeehyeong (Department of Civil Environment Engineering, Korea University) ;
  • Hong, Jongki (Hazardous substance research team, Korea Basic Science Institute)
  • 안윤경 (한국기초과학지원연구원 유해물질분석연구팀) ;
  • 이지연 (한국기초과학지원연구원 유해물질분석연구팀) ;
  • 김지형 (고려대학교 토목환경공학과) ;
  • 홍종기 (한국기초과학지원연구원 유해물질분석연구팀)
  • Received : 2003.10.08
  • Accepted : 2003.11.10
  • Published : 2003.12.25


The HS (headspace)-SPME (Solid phase microextraction) as rapid and simple method was performed for the determination of volatile fatty acids (VFAs) from the aqueous samples. In-fiber derivatization of VFAs with 1-Pyrenyldiazomethane (PDAM) was applied to improve their sensitivity of detection. In SPME procedure, typical parameters such as effects of solution pH, and salting out reagent and ultrasonication were investigated to improve the extraction efficiency. Based on the developed method, VFAs in wastewater samples were determined by gas chromatography / mass spectrometry-selected ion monitoring (GC/MS-SIM) mode.


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