Determination of Volatile Fatty Acids in Aqueous Samples by HS-SPME with In-Fiber Derivatization

Fiber내 유도체화/HS-SPME를 이용한 수용액 시료 중 휘발성 지방산의 분석

  • Ahn, Yun Gyong (Hazardous substance research team, Korea Basic Science Institute) ;
  • Lee, Jee Yeon (Hazardous substance research team, Korea Basic Science Institute) ;
  • Kim, Jeehyeong (Department of Civil Environment Engineering, Korea University) ;
  • Hong, Jongki (Hazardous substance research team, Korea Basic Science Institute)
  • 안윤경 (한국기초과학지원연구원 유해물질분석연구팀) ;
  • 이지연 (한국기초과학지원연구원 유해물질분석연구팀) ;
  • 김지형 (고려대학교 토목환경공학과) ;
  • 홍종기 (한국기초과학지원연구원 유해물질분석연구팀)
  • Received : 2003.10.08
  • Accepted : 2003.11.10
  • Published : 2003.12.25

Abstract

The HS (headspace)-SPME (Solid phase microextraction) as rapid and simple method was performed for the determination of volatile fatty acids (VFAs) from the aqueous samples. In-fiber derivatization of VFAs with 1-Pyrenyldiazomethane (PDAM) was applied to improve their sensitivity of detection. In SPME procedure, typical parameters such as effects of solution pH, and salting out reagent and ultrasonication were investigated to improve the extraction efficiency. Based on the developed method, VFAs in wastewater samples were determined by gas chromatography / mass spectrometry-selected ion monitoring (GC/MS-SIM) mode.

References

  1. Z. H. Huang, C. C. Sweeley, Anal. Chim Acta, 259, 185-192(1992)
  2. A. Vairamurthy, K. Mopper, Anal. Chim Acta, 237, 215-221(1990)
  3. J. G. Andrew, Groen L. A. and M. E. Johnson, J. Chromatogr A., 849, 541-552 (1999)
  4. G. Gmeiner, P. Gartner, C. Krassnig and H. Tausch, J. Chromatogr B., 766, 209-218(2002)
  5. T. Lewis, P. D. Nichols and T. A. McMeekin, Microbiological Methods, 43, 107-116(2000)
  6. O. Pinho, I. M. Ferreira and M. A. Ferreira, Anal. Chern., 74(20), 5199-5204(2002)
  7. M. Mecozzi, M. Amici, G. Romanelli, E. Pietrantonio, and A. Deluca, J.Chromarogr A., 963, 363-373(2002)
  8. D. W. Rhodes, Soil Sci. Am. Proc., 21, 389-392(1957)
  9. A. E. Greenberg, R. R. Trussell and L. S. Clesceri, 'Standard Methods for the Examination of Water and Wastewater', 16th Ed., p. 504, American Public Health Association, Washington, DC, U.S.A., 1985
  10. M. Abalos, J. M. Bayona and J. Pawliszyn, J. Chromatogr A, 873, 107-115(2000)
  11. M. Abalos, J. M. Bayona, J. Chromatogr A., 891, 287-294(2000) https://doi.org/10.1016/S0021-9673(00)00655-5
  12. P. D. Ahlfeld, M. M. John and F. P. George, Water Res. 9, 77-84(1986)
  13. I. Murad, H. Helalh, S. Fujii and T. Korenaga, Talanta, 54, 1039-1047(2001) https://doi.org/10.1016/S0039-9140(01)00386-1
  14. I. Murad, H. Helalh, Y. Takabayashi, S. Fujii and T. Korenaga, Anal. Chim Acta, 428, 227-234(2001) https://doi.org/10.1016/S0003-2670(00)01251-4
  15. M. C. Wentzel, G. A. Ekama and G. Marais, Water Sci. Technol., 23, 555-568(1995)
  16. T. J. Clark, J. E. Bunch, J. Chromatogr. Sci., 35, 206-219(1997)
  17. L. Pan, M. Adams and J. Pawliszyn, 67(23), Anal. Chem., 4396-4403(1995)
  18. G. Manni, F. Caron, J. Chromatogr A., 690, 237-242(1995)
  19. S. H. Pine, 'Organic Chemistry,' 5th Ed., p. 272, McGraw-Hill, New York, U. S. A., 1985
  20. A. Tangerman, F. M. Nagengast, Anal. Biochem., 236, 1-8(1996)
  21. L. Pan and J. Pawliszyn, Anal. Chem., 69(2), 196-205(1997) https://doi.org/10.1021/ac9606362
  22. Wang, Y. Ph. D. Thesis : A study of solid phase microextraction and headspace sampling., University of virginia, 1997.
  23. G. E Bailescu, V. A. Ilie, ' Stationary Phases in Gas Chromatography', B. M. Trost, J. Fleming, Ed., Pergamon Press, Oxford, UK, 1975
  24. J. Schneede, P. M. Ueland, Ana!' Chern., 64, 315-319(1992)
  25. Y. Jianzhen, C. F. Richard and H. S. John, Environ. Sci. Techno!' 32(16), 2357-2370 (1998)
  26. J. A. Cruwys, R. M. Dinsdale, F. R. Hawkes and D. L. Hawkes, J. Chromatogr A., 945, 195-209(2002)