Induction of Cdk inhibitor p27 and Inhibition of pRB Phosphorylation by Insamsapye-tang Treatment in Human Lung Cancer A549 Cells

인체 폐암세포에서 인삼사폐탕에 의한 Cdk inhibitor p27의 발현 증가 및 pRB의 인산화 억제

  • Lee Min Woo (Department of Internal Medicine, College of Oriental Medicine, Dongeui University) ;
  • Seo Chang Hun (Department of Internal Medicine, College of Oriental Medicine, Dongeui University) ;
  • Park Cheol (Biochemistry, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine) ;
  • Lee Won Ho (Department of Biology, College of Natural Sciences, Busan National University) ;
  • Choi Yung Hyun (Biochemistry, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine) ;
  • Park Dong Il (Department of Internal Medicine, College of Oriental Medicine, Dongeui University)
  • 이민우 (동의대학교 한의과대학 폐계내과학교실) ;
  • 서창훈 (동의대학교 한의과대학 폐계내과학교실) ;
  • 박철 (동의대학교 한의과대학 생화학 교실 및 한의학연구소) ;
  • 이원호 (부산대학교 자연과학대학 생물학과) ;
  • 최영현 (동의대학교 한의과대학 생화학 교실 및 한의학연구소) ;
  • 박동일 (동의대학교 한의과대학 폐계내과학교실)
  • Published : 2003.02.01

Abstract

We investigated the effects of Insamsapye-tang (ISSPT) water extract on the cell proliferation of human lung carcinoma A549 cells. ISSPT treatment resulted in the inhibition of cell proliferation in a concentration-dependent manner. This anti-proliferative effect of A549 cells by ISSSPT treatment was associated with morphological changes such as membrane shrinking and cell rounding up. DNA flow cytometric histograms showed that population of G1 phase of the cell cycle was increased by ISSPT treatment in a concentration-dependent manner. ISSPT treatment induced the levels of tumor suppressor p53 protein and cyclin-dependent kinase (Cdk) inhibitor p27 without significant alteration of cyclins and Cdks expression. In addition, ISSPT treatment resulted in down-regulation of phosphorylated retinoblastoma protein (pRB). However, the levels of p130, the pRB family protein, and transcription factors. E2F-1 and E2F-4. were remained unchanged. The present results indicated that ISSPT-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1/S progression and the induction of apoptosis, and we suggest that ISSPT will be an effective therapeutic agent on human lung cancer.

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