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Linkage Map and Quantitative Trait Loci(QTL) on Pig Chromosome 6

돼지 염색체 6번의 연관지도 및 양적형질 유전자좌위 탐색

  • Lee, H.Y. (National Livestock Research Institute, RDA) ;
  • Choi, B.H. (National Livestock Research Institute, RDA) ;
  • Kim, T.H. (National Livestock Research Institute, RDA) ;
  • Park, E.W. (National Livestock Research Institute, RDA) ;
  • Yoon, D.H. (National Livestock Research Institute, RDA) ;
  • Lee, H.K. (Department of Biotechnology, Hankyong National University) ;
  • Jeon, G.J. (Department of Biotechnology, Hankyong National University) ;
  • Cheong, I.C. (National Livestock Research Institute, RDA) ;
  • Hong, K.C. (Division of Biotechnology and Genetic Engineering, College of Life and Environmental Sciences Korea University)
  • 이혜영 (농촌진흥청 축산기술연구소) ;
  • 최봉환 (농촌진흥청 축산기술연구소) ;
  • 김태헌 (농촌진흥청 축산기술연구소) ;
  • 박응우 (농촌진흥청 축산기술연구소) ;
  • 윤두학 (농촌진흥청 축산기술연구소) ;
  • 이학교 (한경대학교 생명공학과) ;
  • 전광주 (한경대학교 생명공학과) ;
  • 정일정 (농촌진흥청 축산기술연구소) ;
  • 홍기창 (고려대학교 생명유전공학부)
  • Published : 2003.12.31

Abstract

The objective of this study was to identify the quantitative traits loci(QTL) for economically important traits such as growth, carcass and meat quality on pig chromosome 6. A three generation resource population was constructed from cross between Korean native boars and Landrace sows. A total of 240 F$_2$ animals were produced using intercross between 10 boars and 31 sows of F$_1$ animals. Phenotypic data including body weight at 3 weeks, backfat thickness, muscle pH, shear force and crude protein level were collected from F$_2$ animals. Animals including grandparents(F$_0$), parents(F$_1$) and offspring(F$_2$) were genotyped for 29 microsatellite markers and PCR-RFLP marker on chromosome 6. The linkage analysis was performed using CRI-MAP software version 2.4(Green et al., 1990) with FIXED option to obtain the map distances. The total length of SSC6 linkage map estimated in this study was 169.3cM. The average distance between adjacent markers was 6.05cM. For mapping of QTL, we used F$_2$ QTL Analysis Servlet of QTL express, a web-based QTL mapping tool(http://qtl.cap.ed.ac.uk). Five QTLs were detected at 5% chromosome-wide level for body weight of 3 weeks of age, shear force, meat pH at 24 hours after slaughtering, backfat thickness and crude protein level on SSC6.

Keywords

QTL Mapping;Resource population;Economic traits;Linkage mapping;Pigs

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