Effects of Blended Essential Oil(CRINA®) Supplementation on the Performance, Nutrient Digestibility, Small Intestinal Microflora and Fatty Acid Composition of Meat in Broiler Chickens

사료중 Blended Essential Oil(CRINA®) 첨가가 육계의 생산성과 영양소 이용률, 소장 내 미생물 균총 및 계육내 지방산 조성에 미치는 영향

  • Suk, J.C. (Department of Animal Science and Technology, College of Industrial Science, Chung-Ang University) ;
  • Lim , H.S. (Department of Animal Science and Technology, College of Industrial Science, Chung-Ang University) ;
  • Paik, I.K. (Department of Animal Science and Technology, College of Industrial Science, Chung-Ang University)
  • 석종찬 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 임희석 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 백인기 (중앙대학교 산업과학대학 동물자원과학과)
  • Published : 2003.10.31


An experiment was conducted to investigate the effects of supplementary Blended essential oil(CRINA$^{\circledR}$) on the performance, nutrient availability, fatty acid composition of leg muscle, small intestinal microflora and blood parameters in broiler chickens. One thousand unsexed day-old broiler chickens were assigned to five treatments : control(T1), 5ppm avilamycin(starter diet) & 5ppm flavomycin(grower diet) T2, 5ppm avilamycin(starter diet) & 50ppm CRINA$^{\circledR}$(grower diet) T3, 50ppm CRINA$^{\circledR}$(starter & grower diet) T4, 50ppm CRINA$^{\circledR}$+ 500ppm lactic acid$^{\circledR}$ (starter & grower diet) T5. Each treatment had four replications of 50 birds each. Growth performance was significantly improved by dietary supplements(T2-T5). There were no significant differences among treatment T2, T3, T4 and T5. Feed intake was not significantly different among treatments. Dietary supplementation of CRINA$^{\circledR}$(T3, T4, T5) resulted in significant(p〈0.05) improvement in feed/gain(F/G) during finishing period (4-5weeks). The birds fed CRINA$^{\circledR}$ supplemented diet(T4) showed significantly(p〈0.05) higher availability of crude fat, methionine and methionine + cystine than those fed antibiotics supplemented diet(T2). Mortality was not significantly affected by treatments. The colony forming unit(CFU) of E.coli in small intestinal content was significantly lower in antibiotics & CRINA$^{\circledR}$(T3) compared to CRINA$^{\circledR}$ treatment(T4)(P〈0.05). CFU of Cl. perfringens was low in CRINA$^{\circledR}$(T4) but not different significantly with other treatments. Serum triglyceride level of birds fed CRINA$^{\circledR}$ + lactic acid diet(T5) was significantly lower(p〈0.05) than those fed antibiotics supplemented diet(T2). Cholesterol level of the birds fed antibiotics(T2) or CRINA$^{\circledR}$ + lactic acid supplemented diet(T5) was significantly higher(p〈0.05) than other treatments. HDL level of birds fed control diet was significantly lower(p〈0.05) than that of others. The levels of serum IgG were not significantly different among treatments. Major fatty acids composition of leg muscle fat was significantly influenced by treatments. Control group showed significantly higher palmitic acid(C$_{16:0}$) and steraric acid(C$_{18:0}$) content than other treatments(p〈0.05). Content of oleic acid(C$_{18:1}$), however, was significantly lower in the control than others treatments. Content of linolenic acid(C$_{18:3}$) was significantly higher in CRINA$^{\circledR}$+ lactic acid(T5) than antibiotics & CRINA$^{\circledR}$(T3) treatments. Total saturated fatty acids content was higher and total unsaturated fatty acids were lower in the leg muscle fat of the control than that of other treatments. It is concluded that CRINA$^{\circledR}$ supplementation improved growth rate and F/G ratio in broilers. The combination of CRINA$^{\circledR}$ with either antibiotics or lactic acid did not show any additive or synergistic effects in broiler chickens .


Essential oil;CRINA$^{\circledR}$;Small intestinal microflora;Fatty acid composition;Growth performance;Broiler


  1. Adams, C. H. 1999. Nutricines Food Components in Health and Ntrition. Nottingham Univ. presses.
  2. Al-Sheikly, F. and Truscott, R. B. 1997. The interaction of and its toxin in the production of necrotic enteritis in chicken. Avian DIS., 21:256- 263.
  3. AOAC. 1990. Official Method of Analysis, 15th ed. Association of Official Analysis Chemist. Washington D. C.
  4. Chang, S. T., Chen, P. F. and Chang, S. C. 2001. Antibioterial activity of leaf essential oil and their constituents from Cinnamomum osmophloeum. J. Ethnopharmacol. 77:123-127.
  5. Charalambous, G. (ed). 1994. Spices, herbs anf edible fungi. Elsevier, London.
  6. Eijk. Corn van der, 2002. Acidifiers and AGP’s compared. Feed mix. Vol.10, No. 6: 34-36.
  7. Gilliland, S. E., Nelson, C. R. and Maxwell, C. 1985. Assimilation of cholesterol by Lactobacilli. Am.Soc.Microbiol. 49:377.
  8. Helander, I. m., Alakomi, H. L., Latva-Kala, K, Mattila-Sandholm., T. I. Pol., Smed, E. J., Gorris, L. G. M., Wrifght, A. von. 1998. Characterization of the action of selected seeential oil components on Gram-Negative bacteria. J. Agric. Food Chem. 46(9):3590-3595.
  9. Jamroz. D. and Kamel, C. 2002. Plant extracts enhance broiler performance. J. Anim. Sic. Vol 80, Suppl. 1:41.
  10. Kamel. C., 2001. Plant extracts in an integrated approach. Feed mix. Vol. 6, No. 6:23-25.
  11. Kamel, C. 2002. Re-defining botanicals more that simple bug killers. Feed international. No 3:24-27.
  12. Kohler, B. 1992. Clostridiosen in Krankheiten des Wirtschetsgefugels(Herausgeber G. Heider and G, Montreal) Bd Ⅱ, 195-234.
  13. Kohler, B. 1997. Bericht der mikrobiologischen Untersuchungen bei der Erprobung der Wirsamkeit von CRINA HC 737 im Vergleich zum kon ventionellen Leistungdforderer auf die Mastleistung beim Broiler unter Praxisbedingungen. CR constract AKZO NOBEL, Staatliches Veterinar-und Lebensmitteluntersuchungsamt Potsdam.
  14. Lepage, G. and ROY, C. C. 1986, J. Lipid res. 27:114-120(AOCS).
  15. Losa, R. and Kohler, B. 2001. Prevention of colonization of Clostridia perfringens in broilers intestine by essential oils. 13th Eur. Symp. Poult. Nutr. Oct. 2001, Blankerberge. Belgium. p. 33-134.
  16. Losa, R. 2002a. Potential of essential oil compounds on animal nutrition. Proceedings '유럽의 사료첨가제 사용추세와 Essential Oil CRINA$^{\circledR}$제제 연구'. 올더베스트, Seoul. Korea.
  17. Losa, R. 2002b. The use of essential oils in animal nutrition. Ⅲ Conference Show Feed Manufacturing Region, Reus, Spain, March 22-24, 2002. pp. 49-54.
  18. Lucy, T. 2002. Botnical broilers: Plant extracts to maintain poultry performance. Feed international. No 9:26-29.
  19. Mitsch, P., Kohler, B., Gabler, C., Losa., R., Zitterleglseer, K. 2002. CRINA$^{\circledR}$ poultry reduces colonisation and proliferation of Clostridium perfringens in the intestine and faeces of broiler chickens. Abstracts 11th European Poultry Conference Bremen. p. 113.
  20. Pruthi, J. S. 1980. Spices and condiments : chemistry, microbiology technology. Advances in food research. Ed. Academic Press Inc. London, UK.
  21. Richard, H.(ed). 1992. Spices and aromats, Lavoisier, Paris.
  22. SAS Instute Inc, 1995. SAS$^{\circledR}$ User's Guide: Statistics. Version 6.12 Edition. SAS Instistute Inc., Cary, NC
  23. Silvia, P. and Asensio, J. 2002. Additive for performance : Organic acids plus botanicals. Feed international. No 3:17-19.
  24. Wang, R. J., Li, D. F. and Steve, B. 1998. Can 2000 years of herbal medicine history help us solve problems in the year 2000. In: Biotechology in the feed industry(Lyons T. P., and K. A. Jacques). Nottingham University Press. pp. 271-291
  25. Wenk, C. 2003. Herbs and botanicals as feed additives in monogastric animals. Asian-Aust. J. Anim. Sci. Vol. 16, No. 2:282-289.
  26. Williams, P. and Losa, R. 2002. Blending essential oils. Feed Mix. Vol. 10, No. 3:8-9.
  27. 국 길, 이종봉, 공흥범, 김광현, 2001. 죽초액의 첨가가 재래닭의 생산성 및 육질에 미치는 영향, Proceeding of the 2001 International Symposium and 18th Annual Meeting of the Korea Society of Poultry Science. p. 141-143.
  28. 이홍률, 류경선. 2001 산란계사료에 목초액의 첨가 급여가 생산성 및 계란품질에 미치는 영향. 동물자원과학회지 43(5):665-662.

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