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Sequence and Expression Analysis of c-fos Proto-oncogene in Korean Cattle (HANWOO)

한우 c-fos 유전자의 염기서열 및 발현분석

  • Yu, S.L. (Division of Animal Science and Resources) ;
  • Chung, H.J. (Division of Animal Science and Resources) ;
  • Jung, K.C. (Division of Animal Science and Resources) ;
  • Lee, J.H. (Division of Animal Science and Resources) ;
  • Cho, K.W. (Department of Veterinary Medicine, Chungnam National University) ;
  • Choi, J.G. (Daekwanryong Branch, National Livestock Research Institute, R.D.A.) ;
  • Na, K.J. (Daekwanryong Branch, National Livestock Research Institute, R.D.A.) ;
  • Sang, B.C. (Division of Animal Science and Resources)
  • 유성란 (충남대학교 동물자원학부) ;
  • 정행진 (충남대학교 동물자원학부) ;
  • 정기철 (충남대학교 동물자원학부) ;
  • 이준헌 (충남대학교 동물자원학부) ;
  • 조규완 (수의학과) ;
  • 최재관 (농촌진흥청 축산기술연구소) ;
  • 나기준 (농촌진흥청 축산기술연구소) ;
  • 상병찬 (충남대학교 동물자원학부)
  • Published : 2003.12.31

Abstract

Cellular FOS(c-fos) protein is a transcription factor that forms heterodimers mostly with c-jun family and stimulates the transcription of genes containing AP-1 regulatory elements. This c-fos expression can control growth and differentiation of various precursor cells including myoblasts. The controls by c-fos gene have been identified for affecting skeletal muscle fiber traits which are the key determinants of meat quality in pigs. As a first step for identifying the relationship between c-fos gene and meat quality traits in cattle, we fully sequenced 1,443 bp of Hanwoo c-fos mRNA and analyzed expression patterns from various organs and muscle tissues. The sequence identities of Hanwoo c-fos with that of human, pig and mouse showed 89.8%, 93.3% and 87%, respectively. Analyses of the northern blot showed high c-fos expressions were obtained in spleen and rib muscle from 7 organs and 9 different parts of muscles investigated. These results presented here can be used as a valuable marker for meat quality related traits in cattle with further verification.

Keywords

Bovine;c-fos;Sequence homology;Expression pattern;Meat quality

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