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Comparative Analysis of Muscle Proteome from Porcine White and Red Muscles by Two-dimensional Electrophoresis

이차원전기영동법을 이용한 white muscle과 red muscle간의 단백질 발현양상의 비교분석

  • Kim, N.K. (Department of Applied Biochemistry) ;
  • Joh, J.H. (Department of Applied Biochemistry) ;
  • Chu, K.S. (Department of Applied Biochemistry) ;
  • Park, H.R. (Department of Applied Biochemistry) ;
  • Park, B.Y. (National Livestock Research Institute, R.D.A.) ;
  • Kim, O.H. (Department of Animal Science, College of Natural Science, Konkuk University) ;
  • Lee, C.S. (Department of Applied Biochemistry)
  • 김남국 (건국대학교 자연과학대학 응용생화학) ;
  • 조중호 (건국대학교 자연과학대학 응용생화학) ;
  • 추교선 (건국대학교 자연과학대학 응용생화학) ;
  • 박혜란 (건국대학교 자연과학대학 응용생화학) ;
  • 박범영 (농촌진흥청 축산기술연구소) ;
  • 김언현 (건국대학교 자연과학대학 축산학과) ;
  • 이창수 (건국대학교 자연과학대학 응용생화학)
  • Published : 2003.10.31

Abstract

The technique known as proteomics is useful for characterizing the protein expression pattern of a particular tissue or cell type as well as quantitatively identifying differences in the levels of individual proteins. In present study, we carried out the comparative expression patterns of white and red muscles. We used the two-dimensional electrophoresis(2-DE) for analyzing the protein expression. Proteins isolated from porcine white and red muscles were separated by 12% poly-acrylamide gel and then were detected by coomassie blue and silver staining. More than 600 protein spots were detected on each 2-DE gel. By visual analysis of the stained gel, five proteins were identified to be differentially expressed in the white vs red muscle. By database searching based on the molecular weights and pI(isoelectric point) of the five proteins, three of them were found to be most close to troponin I, T and myoglobin. However, further researche is needed for identification and functional analysis of the unidentified proteins. In conclusion, we found five proteins, which are differentially expressed in the white vs red muscle. The functional analysis of the differentially expressed proteins will provide valuable information on biochemical characteristics of the muscle type.

Keywords

White and red muscle;Two-dimensional electrophoresis

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