A study of Association of the H-FABP RFLP with Economic Traits of Pigs

돼지 H-FABP 유전자의 다형성 및 경제 형질과의 연관성 구명

  • Choi, B.H. (National Livestock Research Institute, RDA) ;
  • Kim, T.H. (National Livestock Research Institute, RDA) ;
  • Lee, J.W. (National Livestock Research Institute, RDA) ;
  • Cho, Y.M. (National Livestock Research Institute, RDA) ;
  • Lee, H.Y. (National Livestock Research Institute, RDA) ;
  • Cho, B.W. (Department of Animal Science, Miryang National University) ;
  • Cheong, I.C. (National Livestock Research Institute, RDA)
  • 최봉환 (농촌진흥청 축산기술연구소) ;
  • 김태헌 (농촌진흥청 축산기술연구소) ;
  • 이지웅 (농촌진흥청 축산기술연구소) ;
  • 조용민 (농촌진흥청 축산기술연구소) ;
  • 이혜영 (농촌진흥청 축산기술연구소) ;
  • 조병욱 (밀양대학교 동물자원학과) ;
  • 정일정 (농촌진흥청 축산기술연구소)
  • Published : 2003.10.31


The purpose of this study was to detect association between genetic variation and economic trait in the porcine heart type fatty acid-binding protein gene as a candidate gene for the traits related with growth and meat quality in pigs. The H-FABP is a 15-kDa protein expressed in several tissues with high demand for fat metabolism such as cardiac and skeletal muscle and lactating mammary gland. H-FABP is small intracellular protein involved in fatty acid transport from the plasma membrane to the site of $\beta$-oxidation and/or triacylglycerol or phospholipid synthesis. In this study, H-FABP PCR-RFLP was performed in F$_2$ population composed of 214 individuals from an intercross between Korean Native Boars and Landrace sows. PCR products from two primer sets within H-FABP gene were amplified in 850bp and 700bp. Digestion of PCR products with the restriction digestion enzymes HaeⅢ and HinfⅠ, revealed fragment length polymorphisms(RFLPs). The genotype frequencies from H-FABP/HaeⅢ was .29 for genotype DD, .53 for genotype Dd, and .15 for genotype dd, respectively. The genotype frequencies of HH, Hh, and hh from H-FABP/HinfⅠ was .38, .41 and .20, respectively, in the population. Relationships between their genotypes and economic traits were estimated. In H-FABP/HaeⅢ locus, there were specific genotypes(Dd and dd) associated with economic traits such as body weights at 3, 5, 12, and 30 week of age (p〈.05 to .001). The ‘d’ allele was associated with gaining of body weight. In H-FABP/HinfⅠ locus, Genotypes of HH and Hh associated with growth traits such as body weights at 5, 12, and 30 week of age (p〈.05 or p〈.001) and back fat thickness, body fat including abdominal and trimmed fat (p〈.001) and intramuscular fat(p〈.05) The ‘H’ allele was positively associated with gaining of body weight and fatness deposition. In conclusion, a significant association of the H-FABP gene from its genetic variation was found on body weight, intramuscular fat and backfat thickness.


H-FABP;PCR-RFLP;Fat Metabolism;Meat Quality;Korean Native Boar


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