DOI QR코드

DOI QR Code

A study of Association of the H-FABP RFLP with Economic Traits of Pigs

돼지 H-FABP 유전자의 다형성 및 경제 형질과의 연관성 구명

  • Choi, B.H. (National Livestock Research Institute, RDA) ;
  • Kim, T.H. (National Livestock Research Institute, RDA) ;
  • Lee, J.W. (National Livestock Research Institute, RDA) ;
  • Cho, Y.M. (National Livestock Research Institute, RDA) ;
  • Lee, H.Y. (National Livestock Research Institute, RDA) ;
  • Cho, B.W. (Department of Animal Science, Miryang National University) ;
  • Cheong, I.C. (National Livestock Research Institute, RDA)
  • 최봉환 (농촌진흥청 축산기술연구소) ;
  • 김태헌 (농촌진흥청 축산기술연구소) ;
  • 이지웅 (농촌진흥청 축산기술연구소) ;
  • 조용민 (농촌진흥청 축산기술연구소) ;
  • 이혜영 (농촌진흥청 축산기술연구소) ;
  • 조병욱 (밀양대학교 동물자원학과) ;
  • 정일정 (농촌진흥청 축산기술연구소)
  • Published : 2003.10.31

Abstract

The purpose of this study was to detect association between genetic variation and economic trait in the porcine heart type fatty acid-binding protein gene as a candidate gene for the traits related with growth and meat quality in pigs. The H-FABP is a 15-kDa protein expressed in several tissues with high demand for fat metabolism such as cardiac and skeletal muscle and lactating mammary gland. H-FABP is small intracellular protein involved in fatty acid transport from the plasma membrane to the site of $\beta$-oxidation and/or triacylglycerol or phospholipid synthesis. In this study, H-FABP PCR-RFLP was performed in F$_2$ population composed of 214 individuals from an intercross between Korean Native Boars and Landrace sows. PCR products from two primer sets within H-FABP gene were amplified in 850bp and 700bp. Digestion of PCR products with the restriction digestion enzymes HaeⅢ and HinfⅠ, revealed fragment length polymorphisms(RFLPs). The genotype frequencies from H-FABP/HaeⅢ was .29 for genotype DD, .53 for genotype Dd, and .15 for genotype dd, respectively. The genotype frequencies of HH, Hh, and hh from H-FABP/HinfⅠ was .38, .41 and .20, respectively, in the population. Relationships between their genotypes and economic traits were estimated. In H-FABP/HaeⅢ locus, there were specific genotypes(Dd and dd) associated with economic traits such as body weights at 3, 5, 12, and 30 week of age (p〈.05 to .001). The ‘d’ allele was associated with gaining of body weight. In H-FABP/HinfⅠ locus, Genotypes of HH and Hh associated with growth traits such as body weights at 5, 12, and 30 week of age (p〈.05 or p〈.001) and back fat thickness, body fat including abdominal and trimmed fat (p〈.001) and intramuscular fat(p〈.05) The ‘H’ allele was positively associated with gaining of body weight and fatness deposition. In conclusion, a significant association of the H-FABP gene from its genetic variation was found on body weight, intramuscular fat and backfat thickness.

Keywords

H-FABP;PCR-RFLP;Fat Metabolism;Meat Quality;Korean Native Boar

References

  1. AOAC. 1990. Official Methods of Analysis (15th Ed.). Association of Official Analytical Chemists, Washington, DC.
  2. Cameron, N. D. 1990. Comparison of Duroc and British Landrace pigs and the estimation of genetic and phenotypic parameters for growth and carcass traits. Animal Production 50:141.
  3. Clamp, P. A., Beever, J. E., Fernando, R. L., McLaren, D. G. and Schook, L. B. 1992. Detection and linkage between genetic markers and genes that affect growth and carcass traits in pigs. J. Anim. Sci. 70:2695-2706. https://doi.org/10.2527/1992.7092695x
  4. DeVol, D. L., McKeith, F. K., Bechtel, P. J., Novakofski, J., Shanks, R. D. and Carr, T. R., 1988. Variation in composition and palatability traits and relationships between muscle characteristics and palatability in a random sample of pork carcasses. J. Anim. Sci. 66:385-395.
  5. Ellegren, H., Johansson, M., Chowdary, B. P., Marklund, S., Ruyter, D., Marklund, L., Nielsen, P.B., Edfors-Lilja, I., Gustavsson, I., Juneja, R. K. and Andersson, L. 1993. Assignment of 20 microsatellite markers to the porcine linkage map. Genomics 16:431-439.
  6. SAS. 2000. SAS/STAT User's guide. SAS institute Inc., Cary, NC., USA.
  7. Emnett, R., Moeller, S., Irvin, K., Rothschild, M., Plastow, G. and Goodwin, R. 2000. An Investigation into the genetic controls of pork quality. NSIF Proceedings.
  8. Gerbens, F., Jansen, A., Van Erp, A. J. M., Harders, F., Meuwissen, T. H. E., Rettenberger, G., Veerkamp, J. H. and te Pas, M. F. W. 1998. The adipocyte fatty acid-binding protein locus; characterization and association with intramuscular fat content in pigs. Mamm. Genome 9:1022-1026.
  9. Gerbens, F., Rettenberger, G., Lenstra, J. A., Veerkamp, J. H., te Pas, M. F. W. 1997. Characterization, chromosomal localization, and genetic variation of the porcine heart fatty acid-binding protein gene. Mammalian Genome 8:328-322.
  10. Gerbens, F., Van Erp, A. J. M., Harders, F. L., Verburg, F. J., Meuwisen, T. H. E., Veerkamp, J. H. and te Pas, M. F. W. 1999. Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs. J. Anim. Sci. 77:846-853.
  11. Hovenier, R., Kanis, E., Van Asseldonk T. H. and Westerink, N. G. 1992. Genetic parameters of pig meat quality traits in a halothane negative population. Livest. Prod. Sci. 32:309-321. https://doi.org/10.1016/0301-6226(92)90002-L
  12. Janss, L. L. G., Van Arendonk, J. A. M. and Brascamp, E. W. 1994. Identification of a single gene affecting intramuscular fat in Meishan crossbreds using Gibbs sampling. World Congerence on genetics Applied to Livestock Production, Guelph.
  13. Nechtelberger, D., Pires, V., Solknes, J., Stur, I., Brem, G., Mueller, M. and Mueller, S. 2001. Intramuscular fat content and genetic variants at fatty acid-binding protein loci in Austrian pig. J. Anim. Sci. 79:2798-2804.
  14. Tagliaro, C. H., Franco, M. H. L P., Meincke, W. and Silva, G. 1995. Protein phenotypes and productive traits in landrace, large white and duroc swine. Ciencia Rural 25:61-65.
  15. Veerkamp, J. H., Van Kuppevelt, T. H. M. S. M., Maatman, R. G. H. J. and Prinsen, C. F. M. 1993. Structural and functional aspects of cytosolic fatty acid binding proteins. Prostaglandins Leukot Essent Fatty Acids 49:887-906. https://doi.org/10.1016/0952-3278(93)90174-U
  16. Veerkamp, J. H. and Maatman, R. G. H. J. 1995. Cytoplasmic fatty acid binding proteins: their structure and genes. Progr. Lipid Res. 34:17-52. https://doi.org/10.1016/0163-7827(94)00005-7
  17. Vogeli, P., Stranzinger, G., Schneebeli, H., Hagger, C., Kunzi, N. and Gerwig, C. 1984. Relationships between the H and A-O blood types, Phosphohexose isomerase and 6-phosphogluconate dehydrogenase red cell enzyme systems and halothane sensitivity, and economic traits in a superior and an inferior selection line of Swiss Landrace pigs. J. Anim. Sci. 59:1440-1450.
  18. Wood, J. D., Enser, M., Moncrieff, C. B. and Kempster, A. J. 1988. Effects of carcass fatness and sex on the composition and quality of pigmeat. 34th International Congress of Meat Science and Technology. August 29-September 2, Brisbane, Australia, pp 562-564.
  19. 이종문, 박범영, 유영모, 김동훈, 채현석, 안종남, 정석근, 조낙현, 김용곤, 조병림, 윤정철, 김홍원, 정종원, 조만용, 고경철, 이무화, 이인형. 1997. 소 돼지 도체수율 및 육질특성. p119.
  20. 이학교, 전광주. 2002. 돼지의 QTL 검색을 위한 유의적 임계수준(Threshold) 결정. 한국동물자원학회지, 44(1):31-38.

Cited by

  1. Association of FABP3 Genotypes and Carcass Characteristics in Pigs vol.55, pp.6, 2013, https://doi.org/10.5187/JAST.2013.55.6.551