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Identification of Nucleolus Organizer Regions of Korean Cattle Chromosomes by AgNOR Staining

AgNOR 염색법에 의한 한우 염색체의 Nucleolus Organizer Regions 양상 분석

  • Jung , W. (Department of Animal Science and Biotechnology & RAIRC, Jinju National University) ;
  • Sohn, S.H. (Department of Animal Science and Biotechnology & RAIRC, Jinju National University)
  • 정원 (진주산업대학교 동물생명과학과 . 동물생명산업지역협력연구센터) ;
  • 손시환 (진주산업대학교 동물생명과학과 . 동물생명산업지역협력연구센터)
  • Published : 2003.10.31

Abstract

Nucleolus Organizer Regions (NORs) are the specific chromosome sites where ribosomal genes are located and highly expressed. We have applied the AgNOR staining to identify the distribution of NORs in the chromosomes of Korean Cattle. We have also studied the NORs pattern on the cells originated from different breeds, tissues and sex. Peripheral blood from forty-four Korean Cattle and Holstein was cultured for chromosme preparation. The fibroblast culture from biopsied ear skins was also conducted for chromosome analysis. The distribution of NORs was analyzed by sequential Ag staining and G-banding on metaphases of the cells. In Korean Cattle, the NORs are localized on the telomeres of the five chromosome pairs number 2, 3, 4, 11 and 28. The number of NORs per metaphase ranged from 2 to 10 giving a mean value of 5.6. The number of NORs per cell varied among individuals and cells within same individual. The size of NORs also differed in NO-chromosomes. The number of NORs was significantly different between Korean Cattle and Holstein, fibroblasts and lymphocytes, and male and female. However, the distribution and frequency of NORs were similar among the cells regardless of breeds, tissues, and sex.

Keywords

Korean Cattle;AgNOR;NORs;G-banding;Chromosomes

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