효모배양물 첨가 사료가 계사내 유해가스 발생 및 육계의 생산성에 미치는 영향

Influence of Supplemental Dietary Yeast Culture on the Noxious Gas Emission in Broiler Houses and Performance of Broiler Chicks

  • 박재홍 (전북대학교 동물자원과학과 전북대학교 바이오식품 소재개발 및 산업화 연구센터) ;
  • 류명선 (전북대학교 동물자원과학과 전북대학교 바이오식품 소재개발 및 산업화 연구센터) ;
  • 김상호 (축산기술연구소 대전지소) ;
  • 나종삼 (익산대학교 산업연구소) ;
  • 김종승 (익산대학교 산업연구소) ;
  • 류경선 (전북대학교 동물자원과학과 전북대학교 바이오식품 소재개발 및 산업화 연구센터)
  • Park, J. H. (Dept. of Animal Resources and Biotechnology, Research Center for Industrial Developement of Biofood Materials, Chonbuk National University) ;
  • Ryu, M. S. (Dept. of Animal Resources and Biotechnology, Research Center for Industrial Developement of Biofood Materials, Chonbuk National University) ;
  • Kim, S. H. (National Livestock Research Institute) ;
  • Na, C. S. (Institute of Industrial Technology, Iksan National College) ;
  • Kim, J. S. (Institute of Industrial Technology, Iksan National College) ;
  • Ryu, K. S. (Dept. of Animal Resources and Biotechnology, Research Center for Industrial Developement of Biofood Materials, Chonbuk National University)
  • 발행 : 2003.02.28


본 연구는 효모배양물을 육계에 수준별로 급여하여 두 차례의 사양실험을 시행하였다. 각각의 실험에서 처리구는 사료내 효모배양물 수준을 0, 0.1, 0.2, 0.4%로서 전체 4개의 처리구를 두었다. 실험 1에서는 처리구당 60수씩 총 240수를 환경조절형 계사에 수용하였으며, 계사내 유해가스에 미치는 영향을 고찰하였다. 실험 2에서는 처리구당 5반복, 반복당 16수로 80수씩 전체 320수를 이용하였으며, 육계의 생산성, 장내 미생물의 변화, 혈중 콜레스테롤 및 ND 항체가를 측정하였다. 사육 전기 3주간에 급여된 사료의 조단백질과 에너지 수준은 각각 21.5%, ME 3,100kcal/kg으로 하였으며 후기 2주에는 19%, ME 3,100kcal/kg 수준으로 급여하였다. 실험 1에서 효모배양물을 수준별로 급여시 0.4% 급여구에서 암모니아 가스 발생량은 다른 처리구에 비하여 감소하였고, 이산화탄소는 모든 효모배양물 급여구에서 대조구보다 유의하게 낮았다. 실험 2에서 증체량은 효모배양물 0.2% 급여구가 대조구에 비하여 증가하는 경향을 보였으나 통계적인 차이는 없었고, 사료섭취량 또한 처리구간에 차이가 없었다. 사료요구율은 효모배양물 0.2% 급여구가 대조구보다 유의적으로 개선되었다(P<0.05). 효모배양물의 급여가 육계의 장내 미생물에 미치는 영향은 0.1, 0.2% 급여구에서 회장의 E. coli가 대조구와 비교하여 유의하게 감소하였고(P<0.05), 맹장의 Latobacillus는 0.1% 급여구에서 유의하게 증가하였다. 혈중 총 콜레스테롤은 0.1% 급여구에서 유의적인 차이는 없었으나 감소하는 경향을 보였고, LDL-cholesterol 함량은 효모배양물 0.1% 급여구가 대조구에 비하여 유의하게 감소하였다. ND 항체가는 효모배양물의 급여구에서 대조구보다 높아지는 경향을 보였지만 처리구간에 통계적인 차이는 없었다. 본 연구의 결과, 육계 사료에 효모배양물의 급여로 계사내 유해가스를 감소하였으며, 사료요구율이 개선되었다. 그리고 장내 미생물과 콜레스테롤 수준에서도 변화를 나타내었다.

Two experiments were conducted to investigate the effect of dietary supplementation of yeast culture on the performance of broiler chicks and noxious gas emission in broiler houses. Two hundred forty and three hundred and twenty, one day old Cobb male broiler chicks in Expt 1 and Expt 2, respectively were alloted to four treatment levels of yeast culture (0, 0.1, 0.2, 0.4%). To each treatment, 60 birds were assigned in Expt 1 and 5 replicates of 16 birds each were assigned in Expt 2. Basal diets contained 21.5% and 19.0% CP, and 3,100kcal/kg ME for the starting and finishing periods, respectively. Ammonia and $CO_2$ gas emission were detected twice a day for seven days during the five week period of Expt 1. Weight gain, feed intake and feed efficiency were measured for five weeks in Expt 2. Intestinal microbes, blood cholesterol and ND antibody titer were examined at the end of Expt 2. In Expt 1, the concentration of $NH_3$ in the house of birds fed yeast culture tended to be lower than the control. It was significantly lower in the 0.4% yeast culture treatment than the control (P<0.05). $CO_2$ concentration was significantly lower in all yeast culture treatments regardless of its dietary supplemental level than the control (P<0.05). different from others. Feed efficiency (feed/gain), however, was significantly improved in all yeast culture treatments relative to that of the control for starting period (P<0.05) and that of 0.2% yeast culture treatment was significantly lower than those of the control and 0.4% for the overall period. Total number of E. coli in the ileum of birds fed yeast culture at 0.1 and 0.2% was significantly lower than those of the control and 0.4% in the ileum. The CFU of Lactobacillus spp. of birds fed yeast culture at 0.1% was higher in the cecum compared to other treatments (P<0.05). Total cholesterol level of chicks fed 0.1% yeast culture seemed to be lower compared to that of other treatments, whereas LDL-cholesterol level was significantly lower than those of the control and 0.4% treatment. ND antibody titer tended to be higher in the yeast culture treatments than the control, but was not significantly different. The results of these experiments indicated that 0.2% yeast culture may have a potential to reduce the noxious gas emission in broiler houses and maximize the performance of broiler chicks.


  1. Allen, R. M. D. 1991. Ingestion and absorption of carbohydrates and protein. In: L. R. John(Ed) Phusiolgy of the Gastrointestinal Tract. Second Edition. Raven Press. NY. pp.1469.
  2. Beard, C. W., Hopkins, S. R. and Hammond, J. 1975. Preparation of newcastle disease virus hemagglutination-inhibition test antigen. Avian Dis. 19:692.
  3. Brugier, S. and Patte, F. 1975. Antagonisme in vitro entre l’ultra-levure et different germes bacteriens. Med. Paris 45:61.
  4. Buts, J. P., Bernasconi, P., Vaerman, J. P. and Dive, C. 1990. Stimulation of secretory com- ponent of Sacchromyces boulardii. Dig. Dis. Sci. 35:251.
  5. Corthier, G., Lucas, F., Jouvert, S. and Castex, F. 1992. Effect of oral Saccharomyces boulardii treatment on the activity of Clostridium difficile toxins in mouse digestive tract. Toxicon 30:1583.
  6. Crumplen, R., D’Amore T., Panchal, C. J., Russell, I. and Stewart, G. G. 1989. Industrial uses of yeast: Present and Future. Yeast (Special issue) 5:3.
  7. Friedewald, W. T., Levy, R. L. and Fredrickson, D. S. 1972. Estimation of the concentration of low-density lipoprotein chlesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18:1163.
  8. Glade, M. J. and Fist, M. D. 1988. Dietary yeast culture supplementation enhances urea recycling in the equine large intestine. Nutr. Rep. Int. 37:11.
  9. Hibino, S., Tenashima, H., Minani, Z. and Kajita,M. 1974. Enzymatic digestion of yeast in some animals. Single Cell Protein. Proc. of. Int. Sym. Academic Press. N. Y. pp. 93.
  10. Krause, O. G., Richardson, C. R., Castleberry, R. E. and Cobb, C. W. 1989. Biological response of chicks fed sorghum grain based diets with added grain specific enzymes mixture and yeast. (1989) Texas Tech of Agricultural Science, Lubbock. T5.263:7.
  11. Krueger, W. F., Kassongue, A. and Ganguy, R. C. 1990. Effect of yeast added to the diet of broiler on performance to 28 to 49 days of age. Poultry Sci. 69:75(Abstr.)
  12. Line, J. E., Bailey, J. S., Cox, N. A. and Stem, N. J. 1998. Effect of yeast treatment to reduce Salmonella and Campylobacter populations associated with broiler chicks subjected to transport stress. Poultry Sci. 76:1227.
  13. McGinnis, K. 1992. Lacto-Sacc versus bacitracin in broiler starter diets. Biotechnology in the feed industry Proceeding 8th annual symposium. Alltech Publ. Kenturky, pp.49.
  14. Miller, D. L. and Balloun, S. L. 1966. Unidentified growth factor sources in poult starter diets. Poultry Sci. 45:155.
  15. Phillips, W. A. and Von Tungeln, K. L. 1984. Effect of adding yeast culture to the receiving ration of stressed stocker calves. Page 117 in: Anim. Sci. Rep. MP 116. Oklahoma State Univ., Agric. Exp. Station, Stillwater, OK.
  16. Rose, A. H. 1980. Recent research on industrially important strains of Sacchromyces cerevisiae. In: Skinner. F. A. S. M. Passmore and R. R. Davenport(Ed.). Bilology and Activities of Yeast, The society for Applied Bacteriology symposium Series No.9. Academic Press, London. pp.103.
  17. SAS/STAT. 1996. SAS user's guide. release 6.12 edition, SAS lnst. Inc., Cary. NC.
  18. Shin, H. T., Bae, H. D.. Chung, K. W., Kim, Y. K., Shan, J. H. and Lee, S. K. 1990. Evaluation of live yeast culture as source of probiotics for broiler. 5th AAAP:3:1.
  19. Stanley, V. G.. Ojo, R. Woldesenbet, S. and Hutchinson, D. H. 1993. The usc of Sacchrormyces cerevisiae to suppress the effects of aflatoxicosis in broiler chicks. Poultry Sci. 72: 1867.
  20. Steel, R. G. D. and Torrie, J. H. 1980. Principles and Procedure of Statistics, McGraw Hill. New York.
  21. Thayer, R. H., Burkitt, F. F.. Morrison, R. D. and Murry, E. E. 1978. Efficiency of utilization of dietary phoshorus by caged turkey breeder hens when fed rations supplemented with live yeast culture. Okla. Agric. Exp. Stn, Res. Rep. MP-103:173.
  22. Yoshida, M. 1975. Yeast grown on n-paraffin as future poultry feed. World's Poultry Sci, 31:221.
  23. 박대영, 남궁환, 백인기, 2002. Yeast Culture (Saccharomyces cerevisiae, Pichia pastoris)가 육계의 생산성, 소장내 미생물 균총 및 혈청 IgG 농도에 미치는 영향. 동물자원과학회지 44:289.
  24. 이현우, 김인호, 김춘수 1995. 육계에 있어서 활성효모(Saccharomyces cerevisiae)의 급여가 영향소 이용성과 장내 미생물의 변화에 미치는 영향. 가금학회지 22:203.
  25. 이현우, 김인호, 김춘수 1995. 육계에 있어서 활성효모(Saccharomyces cerevisiae)의 급여가 영향소 이용성과 장내 미생물의 변화에 미치는 영향. 가금학회지 22:203.

피인용 문헌

  1. Effects of Dietary Pro-biotics and Immunomodulator as an Alternative to Antibiotics in Korean Native Chicken vol.53, pp.5, 2011,