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Effect of Sequence Variation in Bovine Mitochondrial DNA D-loop Region on Economic Traits for Hanwoo

한우 경제형질에 미치는 Mitochondrial DNA D-loop 영역의 염기서열 변이효과

  • Oh, J.D. (Genomic Informatics Center, Hankyong National University) ;
  • Yoon, D.H. (National Livestock research Institute) ;
  • Kong, H.S. (Genomic Informatics Center, Hankyong National University) ;
  • Lim, H.J. (Genomic Informatics Center, Hankyong National University) ;
  • Lee, H.K. (Genomic Informatics Center, Hankyong National University) ;
  • Cho, B.W. (Department of Animal Science, Miryang National University) ;
  • Hong, K.C. (College of Life & Environmental Science, Korea University) ;
  • Jeon, G.J. (Genomic Informatics Center, Hankyong National University)
  • 오재돈 (국립한경대학교 유전정보연구소) ;
  • 윤두학 (축산기술연구소) ;
  • 공홍식 (국립한경대학교 유전정보연구소) ;
  • 임현진 (국립한경대학교 유전정보연구소) ;
  • 이학교 (국립한경대학교 유전정보연구소) ;
  • 조병욱 (밀양대학교 동물자원학과) ;
  • 홍기창 (고려대학교 생명유전공학부) ;
  • 전광주 (국립한경대학교 유전정보연구소)
  • Published : 2003.12.31

Abstract

This study was performed to analyse the sequences of variations of mtDNA D-loop and their effects on carcass traits in Hnawoo(Korean cattle). The resulting sequences were compared with previously published sequences for other cattle breeds(GenBank J01394). The PCR was used to amplify a total of 964 bp between nucleotide 15758 and 383 within D-loop region of mtDNA using specific primers. Twenty five polymorphic sites by nucleotide substitution were found in mtDNA of Hanwoo. The frequencies of positions at 169, 16042, 16093, 16119, 16255 and 16302 nt with high levels of sequence polymorphism were 0.891, 0.117, 0.109, 0.182, 0.197 and 0.117, respectively. The substitution effect at 169 and 16119 nt was found significant on marbling score. Also substitution effect at 169 and 16042 nt was highly significant(p〈0.01) on backfat. thickness. Polymorphism of mtDNA sequence in D-loop region could be useful for the analysis of cytoplasmic genetic variation and associations with the other economically important traits and maternal lineage analysis in Hanwoo.

Keywords

mtDNA;D-loop region;Sequence variation;Hanwoo

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