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A Missense Mutation in Exon 5 of the Bovine Growth Hormone Gene

소 성장호르몬 유전자의 Exon 5번에서의 새로운 다형성 연구

  • Yoon, D. H. (National Livestock Research Institute, R.D.A.) ;
  • Kim, T. H. (National Livestock Research Institute, R.D.A.) ;
  • Lee, K. H. (National Livestock Research Institute, R.D.A.) ;
  • Park, E. W. (National Livestock Research Institute, R.D.A.) ;
  • Lee, H. K. (Department of Genomic Engineering, Hankyoung National University) ;
  • Cheong, I. C. (National Livestock Research Institute, R.D.A.) ;
  • Hong, K. C. (Department of College of Life & Environmental Science)
  • 윤두학 (농촌진흥청 축산기술연구소) ;
  • 김태헌 (농촌진흥청 축산기술연구소) ;
  • 이경희 (농촌진흥청 축산기술연구소) ;
  • 박응우 (농촌진흥청 축산기술연구소) ;
  • 이학교 (한경대학교 생명공학과) ;
  • 정일정 (농촌진흥청 축산기술연구소) ;
  • 홍기창 (고려대학교 생명유전공학부)
  • Published : 2003.02.28

Abstract

Growth Hormone (GH) gene is a member of gene family through the evolutionary process from a small common ancestral gene by a series of gene duplications. The role of the GH in growth and performance controls has been extensively studied in human, mice and livestock. Many researchers have considered GH as a strong candidate gene for evaluation of genetic polymorphisms that could be associated with economic traits in cattle. We report here a novel missense mutation within the exon 5 of the bovine Growth Hormone (bGH) gene. We could amplified 522 bp fragments from eight unrelated Hanwoo cattle by PCR, then, subsequently cloned and sequenced. An Msp I RFLP corresponding to a C to T transition was observed at position 2258 nt. From this result, we could predict a missense mutation (Arg to Trp) at codon 166 in a highly conserved region among many mammals. Codominant Mendelian segregation of the two alleles, Msp I (+) and Msp I (-), was observed in two full-sib F2 families (n = 32, African taurine Bos taurus ${\times}$ African zebu Bos indicus) and eight half-sib Hanwoo families. For the availability of genetic marker, we have performed PCR-RFLP with a large number of individual animals from 15 different cattle breeds (European and Asian taurines, and African indicines). Consideration of breed frequencies of Msp I (-) allele in relation to breed type and their geographic origins, shows higher frequencies in humped breeds or Asian cattle breeds than in humpless or European breeds. This result indicates that the missense mutation can be contributed the functional significance such as the signal transduction through the receptor binding, also may be used as a marker for selection of the economic traits in Hanwoo.

Keywords

Bovine Growth Hormone Gene;PCR-RFLP;Missense mutation

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