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G-, C-, and NOR-banding of Korean Native Pig Chromosomes

한국재래돼지의 G-, C-, 및 NOR-banding

  • Sohn, S.H. (Department of Animal Science and Biotechnology & RAIRC, Jinju National University) ;
  • Kweon, O.S. (National Livestock Research Institute, R. D. A.) ;
  • Baik, K.H. (Department of Animal Science and Biotechnology & RAIRC, Jinju National University) ;
  • Jung, W. (Department of Animal Science and Biotechnology & RAIRC, Jinju National University) ;
  • Cho, E.J. (Department of Animal Science and Biotechnology & RAIRC, Jinju National University) ;
  • Kang, M.Y. (Department of Animal Science and Biotechnology & RAIRC, Jinju National University)
  • 손시환 (진주산업대학교 동물생명과학과 . 동물생명산업지역협력연구센터) ;
  • 권오섭 (농진청 축산기술연구소) ;
  • 백규흠 (진주산업대학교 동물생명과학과 . 동물생명산업지역협력연구센터) ;
  • 정원 (진주산업대학교 동물생명과학과 . 동물생명산업지역협력연구센터) ;
  • 조은정 (진주산업대학교 동물생명과학과 . 동물생명산업지역협력연구센터) ;
  • 강민영 (진주산업대학교 동물생명과학과 . 동물생명산업지역협력연구센터)
  • Published : 2003.12.31

Abstract

Using the G-, C-, and NOR-banding techniques, a karyotyping for Korean Native Pig was performed. Blood samples were collected from 50 male Korean Native Pigs that had been bred at the National Livestock Research Institute and then blood cells were prepared from in vitro cultures followed by karyotyping; G-, C-, and NOR-banding patterns of metaphase chromosomes were analyzed. The karyotype of Korean Native Pig is 38, XX or XY which consists of 5 pairs of submetacentric chromosomes(Group I), 2 pairs of acrocentric chromosomes with short p-arm(Group II), 5 pairs of medium metacentric chromosomes(Group III), 6 pairs of acrocentric chromosomes(Group IV) and metacentric X and Y sex chromosomes. On GTG-banding, the Korean Native Pig exhibited a typical and identical banding pattern in each homologous chromosomes. Overall chromosomal morphology and positions of typical landmarks of the Korean Native Pig were virtually identical to those of Committee for the Standardized Karyotype of the Domestic Pig(CSKDP). However, numbers of G-bands of the Korean Native Pig chromosomes were more than those of CSKDP. In chromosomes 1, 3, 5, 6, 7, 8, 13, 14, 15, 16, 17, 18 and X, the Korean Native Pig exhibited more separated bands as compared with CSKDP. In C-banding patterns, although the quantity of heterochromatin was variable in each chromosome, most of the Korean Native Pig chromosomes had heterochromatic C-bands on centromeres. However, the heterochromatic C-band was constantly observed on the whole Y chromosome. In AgNOR staining, the NORs were located at centromeres on the chromosomes 8 and 10. The number of NORs per metaphase ranged from 2 to 4 giving a mean value of 2.13. The number of NORs were distributed on all chromosome pair 10 but not on chromosome 8. The sizes of NORs were also differed between homologous chromosomes 8. Numbers of NORs of Korean Native Pig were significantly higher than those of Yorkshire. The pattern of pig NORs was polymorphic in breeds, individuals and cells, especially on chromosome 8.

Keywords

Korean Native Pig;G-banding;C-banding;NORs;Chromosomes

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