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Effects of the Artificial Culture Medium of Wild Ginsengs on Rumen Fermentation Characteristics In Vitro

산삼배양액 이용에 관한 반추위 미생물 대사 연구

  • Bae, G.S. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Nam, K.P. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Kim, H.S. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Lee, S.G. (Neobio Inc.) ;
  • Choi, H.S. (OOSOO.com Inc.) ;
  • Min, W.K. (Institute of Food Resource, Chung-Ang University) ;
  • Joo, J.W. (Department of Nutritional Resources, Konkuk University) ;
  • Maeng, W.J. (Department of Nutritional Resources, Konkuk University) ;
  • Chang, M.B. (Department of Animal Science & Technology, Chung-Ang University)
  • 배귀석 (중앙대학교 동물자원과학과) ;
  • 남경표 (중앙대학교 동물자원과학과) ;
  • 김혜숙 (중앙대학교 동물자원과학과) ;
  • 이상구 ((주)네오바이오) ;
  • 최행석 ((주)우수컴) ;
  • 민우기 (중앙대학교 식량자원연구소) ;
  • 주종원 (건국대학교 영양자원학과) ;
  • 맹원재 (건국대학교 영양자원학과) ;
  • 장문백 (중앙대학교 동물자원과학과)
  • Published : 2003.12.31

Abstract

This study was conducted to determine the effects of the artificial culture medium of wild-ginsengs on in vitro fermentation characteristics. NH$_3$-N concentration was showed the highest in 3% WGM treatment among all treatments and control. In addition, microbial protein synthesis was significantly different in all treatments throughout the incubation time, and WGM 3% treatment was the highest at the 9 h incubation(P〈0.05). Protozoa numbers within rumen were decreased in all WGM treatments at 9 h incubation time, whereas WGM 3% treatment was always decreased throughout the incubation(P〈0.05). NDF and ADF digestibility were proportionally increased as the incubation time in both control and treatments. NDF digestibility showed no significantly difference between control and the 3% treatment, and ADF digestibility was similar in all. Total volatile fatty acid(VFA) concentrations of WGM treatments without 5% were significantly higher than control (P〈0.05). No differences were observed in total VFA, acetate, propionate and butyrate concentration among the WGM treatments. Acetate/Propionate ratio of WGM treatments was higher than control after 12 h incubation(P〈0.05). As a result of the artificial culture medium of wild-ginseng on rumen fermentation characteristics in vitro, microbial protein synthesis of WGM treatment was higher than control, and WGM 3% was the highest in all treatments(P〈0.05). The effect of saponin in artificial culture medium of wild-ginseng tended to decrease NH$_3$-N concentration, while it increases the microbial synthesis in early incubation. Therefore, artificial cultures medium of wild-ginseng can increase utilization of feed by microbial and anti-protozoal effects of saponin, which may enhance microbial synthesis capacity in early fermentation period in rumen.

Keywords

Artificial culture;WGM;Wild-ginseng;in vitro;Saponin;Microbial protein

References

  1. Allison, M. J. 1970. Nitrogen metabolism of ruminal micro-organisms. In Physiology of Digestion and Metabolism in the Ruminant. Ed. A. T. Phillipson. Oriel Press, Newcastle-upon-Tyne. pp. 456-473.
  2. AOAC. 1990. Official method of analysis, 15thed. Association of Official Analytical Chemists. Washington D. C.
  3. Bird, S. H., Hill, M. K. and Leng, R. A. 1979. The effects of defaunation of the rumen on the growth of lambs on low-protein-high-energy diets. The Br. J. Nutr. 42: 81-87. https://doi.org/10.1079/BJN19790091
  4. Bryant and Robinson, 1963. Apparent incorporation on ammonia and amino acid carbon during growth of selected species of ruminal bacteria. J. of Dairy Sci. 46:150-157.
  5. Cheney, A. L. and Marbach, E. P. 1962. Modified regents for determination of urea and ammonia. Clin. Biochem. 8:130-132.
  6. Demeyer, D. I. and Van Nevel, C. J. 1979. Effect of defaunation on the metabolism of rumen micro-organisms, The Br. J. Nutr. 42:515-524. https://doi.org/10.1079/BJN19790143
  7. Erwin, E. S., Marco, S. J. and Emery, E. M. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44:1768-1771. https://doi.org/10.3168/jds.S0022-0302(61)89956-6
  8. Freeland, W. J., Calcott, P. H. and Anderson, L. R., 1985. Tannins and saponins: interaction in herbicore diets. Biochem. Syst. Ecol. 13:189-193.
  9. Goodall, S. R. 1980. The effect of sarsaponin on digestion and feedlot performance of steers fed high grain diets. Feedstuffs 52(35):24-28.
  10. Goodall, S. R., Braddy, P., Horton, D. and Beckner, B. 1982. Steam flaked vs. high moisture content rations with and without sarsasaponin for finishing steers. Proc. Ann. Meet. Amer. Soc. Anim. Sci. West. Sect. 33:45.
  11. Hespell. R. B. and Bryant, M. P. 1979. Efficiency of rumen microbial growth: Influence of some theoretical and experimental factors on YATP. J. Anim. Sci. 49:1640-1659. https://doi.org/10.2527/jas1979.4961640x
  12. Hill, J. and Leaver, J. D. 1991. Effect of stage of growth and urea addition on the preservation and nutritive value of whole crop wheat. Anim. Prod. 52:606-618.
  13. Hipkins, V. D., Tsai C. H. and Strauss S. H. 1990. Sequence of the gene for the large subunit of ribulose 1, 5-bisphosphate carboxylase from a gymnosperm, Douglas-fir Plant Mol. Biol. 15:505-507.
  14. Ikedo, S., Shimoyamada, M. and Watanabe, K. 1996. Interaction between bovine serum albumin and saponin as studied by heat stability and protease digestion, J. Agric. Food Chem. 44:792-795.
  15. Kil. J. J., Cho, N. K., Kim. B. S., Lee, S. R. and Maeng, W. J. 1994. Effects of yucca extract addition on in vitro fermentation characteristics of feeds and faeces, and on the milk yields in lactating cows. Korean J. Anim. Sci. 36: 698 709.
  16. Kovacs. P. L., Sudekum, K. -H. and Stangassinger, M. 1998. Effects of intake of a mixed diet and time postfeeding on amount and fibre composition of ruminal and faecal particles and on digesta passage from the reticulo-rumen of steers, Anim. Feed Sci. Technol. 71:325-340.
  17. Lowry, O. H., Rosebrough, N. J., Farr, R. L. and Randall. R. J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265-275.
  18. Lloyd, G. and McCown, B. 1980. Combined Proceedings of the International Plant Propagators Society. 30:421-427.
  19. Makkar, H. P. S., Blummel, M., Borowy, N. K. and Becker, K., 1993. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. J. Sci. Food Agric. 61:161-165.
  20. Makkar. H. P. S. and Becker, K. 1996. Notional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaves. Anim. Feed Sci. Technol, 63:211-228.
  21. Makkar, H. P. S., Borowy, N., Becker, K. and Degen, R. 1995. Some problems in fiber determination in tannin-rich forages. Anim. Feed Sci. Technol. 55: 67-76. https://doi.org/10.1016/0377-8401(95)00793-M
  22. Makkar, H. P. S., Blummel, M. and Becker, K. 1997c. Application of an in vitro gas method to understand the effects of natural plant products on availability and partitoning of nutrients. In: Proceedings of the BSAP Occasional Meeting on In Vitro Techniques for Measuring Nutrient Supply to Ruminants, July 8-10, Reading, UK.
  23. Makkar, H. P. S., Singh, B. and Dawra, R. K. 1988. Effect of tannin-rich leaves of oak on various microbial activities of the rumen. Br. J. Nutr. 60: 287-296. https://doi.org/10.1079/BJN19880100
  24. Mehrez, A. Z., Orskov, E. R. and Mcdonald, I. 1977. Rates of rumen fermentation in relation to ammonia concentration. Br. J. Nutri. 38:437-443.
  25. McDougall. E. I. 1948. Studies on ruminant saliva. 1. The composition and output of sheep’s saliva. Biochem. J. 43:99-109.
  26. Ogimoto, K. and Soichi, I. 1981. Atlas of rumen microbiology. Japan Sci. Societies Press. Tokyo. Page:161.
  27. Owen H. R. and Miller A. R. 1992. An examination and correction of plant tissue culture basal medium formulations. Plant Cell, Tissue and Organ Culture 28:147-150.
  28. Pell, A. N., Woolston, T. K., Nelson, K. E. and Schofield, P. 2000. Tannins: biological activity and bacterial tolerance. In: Brooker, J. D. (Ed.), Tannins in Livestock and Human Nutrition. Proceedings of the International Workshop, Adelaide, Australia, May 31-June 2, 1999. ACIAR Proceedings No. 92, pp. 111-116.
  29. SAS User’s Guide:Statistics, release. 8.1 version Edition, 2000. SAS Inst. Cary, NC.
  30. Shinozaki, K., Ohme, M., Tanaka, M., Wakasugi, T., Hayashida, N., Matsubayashi, T., Zaita, N., Chunwongse, J., Obokata, J., Yamaguchi Shinozaki, K., Ohta, C., Torazawa, K., Meng, B. Y., Sugita, M., Deno, H., Kamogashira, T., TYamada, K., Kusuda, J., Takaiwa, F., Kato, A., Tohdoh, N., Shimada, H. and Sugiura, M. 1986. The Complete nucleotide sequence of tobacco chloroplast genome its gene organization and expression. EMBO. J. 5:2043-2049.
  31. Steel, R. G. D. and Torrie, J. H. 1981. Principles and procedures of statistics, 2nd ed. McGraw-Hill, New York.
  32. Van Soest, P. J., Robertson, J. D. and Lewis, B. A. 1991. Methods for dietary, fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  33. Van Der Merwe, M., Winfeld, M. O., Arnold, G. M. and Parker, J. S. 2000. Spatial and temporal aspects of the genetic structure of Juniperus communis populations. Molecular Ecology 9:379-386. https://doi.org/10.1046/j.1365-294x.2000.00868.x
  34. Wang, Y., Waghorn, G. C., Barry, T. N. and Shelton, I. D., 1994. The effect of condensed tannins in Lotus corniculatus on plasma metabolism of methionine, cystine and inorganic sulphate by sheep. Br. J. Nutr. 72:923-937.
  35. Wang, Y., Douglas, G. B., Waghorn, G. C., Barry, T. N. Foote, A. G. and Purchas, R. W. 1996a. Effect of condensed tannins upon the performance of lambs grazing Lotus corniculatus and Lucerne (Medicago sativa). J. Agric. Sci. (Camb.) 126:353-362.
  36. Wang. Y., McAllister, T. A., Newbold, C. J., Rode, L. M., Cheeke, P. R. and Cheng, K -J. 1998. Effects of Yucca schidigera extract on fermentation and degradation of steroidal saponins in the rumen simulation technique (RUSITEC). Anim. Feed Sci. Technol. 74:143-153.
  37. Wallace, R. J., Arthaud, L. and Newbold, C. J. 1994. Influence of Yucca shidigera extract on ruminal ammonia concentrations and ruminal microorganisms, Applied and Environmental Microbiology. 60:1762-1767.
  38. Wallace, R. J. and McPherson, C. A. 1987. Factors affecting the rate of breakdown of bacterial protein in rumen fluid. The Br. J. Nutr. 58:313-323. https://doi.org/10.1079/BJN19870098
  39. 김기현, 김현진, 주종원. 1994. 건조 인삼박의 Alfalfa 대체수준이 반추위 발효성상에 미치는 영향. 한영사지 18:481-490.
  40. 최상숙. 1991. 반추가축에 있어서 한약제 부산물의 사료가치에 관한 연구. 건국대학교 대학원 석사학위 논문.