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Identification and Characteristics of a Purple, Non-Sulfur Bacterium, Rhodobacter sp. EGH-24 from Korea Coast

한국 해안으로부터 Purple, Non-Sulfur Photosynthetic Bacterium, Rhodobacter sp. EGH-24의 분리 및 특성

  • 차미선 ;
  • 김기한 ;
  • 조순자 ;
  • 이나은 ;
  • 이정은 ;
  • 이재동 ;
  • 박재림 ;
  • 이상준
  • Published : 2003.12.01

Abstract

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the 47 point at west and south coast of Korea in September 2001. Separated 13 samples of changes with red color under 28-32$^{\circ}C$, 3000 lux, anaerobe conditions for 7 days cultivated in basal medium. For pure isolation from 13 samples, we used agar-shake tube method (0.4 % agar) and separated 5 strains through 13-repetition test. EGH-24 and EGH-30 was identified as the same strain through the RAPD(Random Amplified Polymorphic DNA)-PCR of strain EGH-9, EGH-13, EGH-23, EGH-24, EGH-30. Four isolates cultivated in synthesis wastewater for wastewater biodegradation test. EGH-24 was selected with efficient wastwater treating strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 16S-rDNA phylogenetic analysis, EGH-24 strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.

Keywords

Photosynthetic bacterium;PHB;RAPD-PCR;Rhodobacter

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