Effect of γ-Irradiation on the Physicochemical Properties of Zein Films

Abstract

To elucidate the effect of gamma-irradiation on the physicochemical properties of zein films, the molecular and mechanical properties of the films were examined after irradiation at various irradiation doses. Gamma-irradiation of zein solutions caused the disruption of the ordered structure of the zein molecules, as well as degradation, cross-linking, and aggregation of the polypeptide chains based on an SDS-PAGE study. Gamma-irradiation increased the solubility of zein and decreased the viscosity due to cleavage of the polypeptide chains. Protein solubility of the zein films in urea/2-mercaptoethanol also increased with increasing irradiation doses. Alterations of the zein molecules by irradiation decreased water vapor per-meability by 12% and increased the elongation of zein films. However, mean tensile strength of the zein films was decreased by gamma-irradiation treatment. Measurement of Hunter color values indicated that irradiation caused a destructive effect on yellow pigments, resulting in a significant decrease in Hunter b values. The microstructure as observed by scanning electron microscopy showed that irradiated zein film had a smoother and glossier surface than the non-irradiated films.

Keywords

• zein film;
• irradiation;
• physicochemical properties

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References

1. J Dairy Sci v.78 Functional properties and applications o edible film made of milk proteins Chen H https://doi.org/10.3168/jds.S0022-0302(95)76885-0
2. Food Technol v.51 Edible and biodegradable polymer films: challenges and opportunities Krochta JM;De Mulder-Johnston C
3. J Agric Food Chem v.48 Mechanical and structural properties of milk protein edible films cross-linked by heating and gamma irradiation Vachon C;Yu HL;Yeash R;Alain R;St-Gelais D;Lacroix M https://doi.org/10.1021/jf991055r
4. In Edible coating and films to improve food quality Permeability properties of edible films McHung TH;Krochta JM;Baldwin E(ed.);Krochta JM(ed.);Nisperos-Carriedo MO(ed.)
5. J Protein Chem v.9 An immunodominant site of $\gamma$-zein is in the region of tandem hexapeptide repeats Esen A https://doi.org/10.1007/BF01024621
6. J Agric Food Chem v.47 Rheology of zein solutions in aqueous ethanol Fu D;Weller CL https://doi.org/10.1021/jf9811121
7. Int J Food Sci Technol v.30 Improved water resistance in edible zein films and composites for biodegradable food packaging Yamada K;Takahahi H;Noguchi A
8. Lebensm-Wiss U-Technol v.32 Properties of ultraviolet irradiated protein films Rhim JW;Gennadios A;Fu D;Weller CL;Hanna MA https://doi.org/10.1006/fstl.1998.0516
9. In Food chemisry Amino acids, peptides, and proteins Chefel JC;Cuq JL;Lorient D;Fennema OR(ed.)
10. Int J Radiat Biol v.45 Oxygen effect in the radiolysis of proteins Shuessler H;Schilling K https://doi.org/10.1080/09553008414550381
11. Int J Radiat Biol v.72 Lysozyme fragmentaion induced by $\gamma$-radiolysis Filali-Mouhim A;Audette M;St-Louis M;Thauvette L;Denoroy L;Penin F;Chen X;Rouleau N;Le Carer JP;Rossier J;Potier M;Le Maire M. https://doi.org/10.1080/095530097143545
12. J Biochem Mol Biol v.33 Effect of $\gamma-irradiation on the molecular properties of BSA and $\beta-lactolobulin Cho Y;Song KB
13. Chem Rev v.87 Reaction mechanism in the radiolysis of peptides, polyptides, and proteins Garrison WM https://doi.org/10.1021/cr00078a006
14. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemli UK https://doi.org/10.1038/227680a0
15. Anal Biochem v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford MM https://doi.org/10.1016/0003-2697(76)90527-3
16. In Annual Book of ASTM Standards Standard test methods for tensile properties of thin plastic sheeting (D 882-91) ASTM
17. In Annual Book of ASTM Standards Standard test methods for water vapor transmission of materials. (E 96-95) ASTM
18. J Food Eng v.25 Gas and water vapor barrier properties of edible films from protein and cellulosic materials. Pakr HJ;Manjeet SC https://doi.org/10.1016/0260-8774(94)00029-9
19. Lebensm-Wiss u-Technol v.35 Preparation and physical properties of zein-coated high-amylose corn starch film Ryu SY;Rhim JW;Roh HJ;Kim SS https://doi.org/10.1006/fstl.2002.0929
20. J Agric Food Chem v.49 Extraction and solubility characteristics of zein proteins from dry-milled corn Parris N;Dickey LC https://doi.org/10.1021/jf0011790
21. Biochem J v.267 Effects of ionizing radiations on proteins Le Maire M;Thauvette L;De Foresta B;Viel A;Beauregard G;Potier M
22. Nutraceut Food v.7 Effect of gamma-irradiation on the molecular properties of blood plasma proteins Lee SH;Lee SH;Song KB https://doi.org/10.3746/jfn.2002.7.2.184
23. J Biol Chem v.262 Protein damage and degradation by oxygen radicals III Davies KJA;Delsignore ME
24. Food Chem v.82 Effect of gamma-irradiation on the physicochemical properties of porcine and bovine blood plasma proteins Lee SH;Lee SH;Sog KB https://doi.org/10.1016/S0308-8146(02)00592-7
25. J Agric Food Sci v.43 Composition factors affecting the water vapor permeability and tensile properties of hydrophilc film Parris N;Coffin DR;Joubran RF;Pessen H
26. Radiat Phys Chem v.62 Use of $\gamma-irradiation cross-linking to improve the water vapor permeability and the chemical stability of milk protein films Ouattra B;Canh LT;Vachon C;Mateescu MA;Lacroix M

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