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Determination of Vitamin B12 (Cyanocobalamin) in Fortified Foods by HPLC

  • Park, Youn-Ju (Division of Nutrition, Korea Food and Drug Administration) ;
  • Jang, Jae-Hee (Division of Nutrition, Korea Food and Drug Administration) ;
  • Park, Hye-Kyung (Division of Nutrition, Korea Food and Drug Administration) ;
  • Koo, Yong-Eui (Division of Nutrition, Korea Food and Drug Administration) ;
  • Hwang, In-Kyeong (Department Food and Nutrition, Seoul National University) ;
  • Kim, Dai-Byung (Division of Nutrition, Korea Food and Drug Administration)
  • Published : 2003.12.01

Abstract

This study was conducted to develop an HPLC method for determining vitamin B$_{12}$ in fortified foods which has typically been determined by microbiological assays according to AOAC and Korean Food Code approved methods. Vitamin B$_{12}$ (cyanocobalamin) was determined by reversed-phase HPLC with a triple column and UV/VIS dectector (550 nm) using the column switching technique after extraction with 5 mM potassium phosphate solution by sonication without a clean-up procedure. The recovery of spiked samples and limit of detection (LOD) by HPLC were 78.6 ∼107.5 % and 2 ppb ($\mu\textrm{g}$/kg), respectively. The LOD of the microbiological assay (MBA) was much lower than that of HPLC. The concentrations of vitamin B$_{12}$ analyzed in all tested samples (n=12) confirmed compliance with declared label claims. The range of recovery ratio by the HPLC method when compared to the microbiological assay was 76.2 ∼140.0 %. There was not significant difference between the HPLC and MBA methods (p < 0.01) with r=0.9791 and linear regression y=0.9923x-0.04. The HPLC method for determining vitamin B$_{12}$ using the column-switching technique appears to be suitable for determining vitamin B$_{12}$ concentrations above 1 $\mu\textrm{g}$/100 g in fortified foods.ied foods.

References

  1. Nutritional Biochemistry Tom B
  2. RDA for Koreans The Korean Nutrition Society
  3. 21st Century Nutrition Choi HM
  4. Vitamin Analysis for the Health and Food Sciences Eitenmiller RR;Landen WO Jr.
  5. Korean food code Korea food & drug daministration
  6. Korean food code(attend.) Korea food & drug administration
  7. Association of official analytical chemists v.2 Official Methods Analysis, (16th ed.) AOAC
  8. J Chromatogr v.771 Determination of cyanocobalamin in foods by high-performance liquid chromatography with visible detection after solid-phase extraction and membrance filtration for the precolumn separation of lipophilic species. Iwase H;Ichiro O https://doi.org/10.1016/S0021-9673(97)00171-4
  9. J Chromatogr v.590 Ultramicrodetermination of cyanacobalamin in elemental diet by solid-phase extraction and high-performance liquid chromatography with visible detection Iwase H https://doi.org/10.1016/0021-9673(92)85399-E
  10. J Chromatogr v.174 High performance liquid chromatographic separation of cobalamins Frankel EP;Kitchens RL;Prough R https://doi.org/10.1016/S0021-9673(00)86013-6
  11. Analyst v.112 Simultaneous determination of vitamin $B_1$, B_2$, $B_6$and $B_12$ and C, nicotinamide and folic acid in capsule preparations by ion-pair reversed-phase high-performance liquid chromatography Amin M;Reusch J https://doi.org/10.1039/an9871200989
  12. J Chromatogr v.390 High performance liquid chromatography of water-soluble vitamins. II. Simultaneous determination of vitamins$B_1$, B_2$, $B_6$, and $B_12$ in pharmaceutical preparations Amin M;Reusch J https://doi.org/10.1016/S0021-9673(01)94398-5
  13. J Pharm Biomed Anal v.20 Determination of cyanocobalmin, betamethason, and diclofenac sodium in pharmaceutical formulation, by high performance liquid chromatography Gonzalez L;Yuln G;Volonte MG https://doi.org/10.1016/S0731-7085(99)00046-1
  14. Analysis Chimica Acta v.395 Determination of cobalamins and cobinamides by microbore reversed-phase HPLC with spectrophotometric, ion-spray ionizaion MS and inductively coupled plasma MS detection. Hubert C;Ryszard L
  15. J AOAC International v.67 Determination of pantothenic acid, biotin, and vitamin $B_12$ in nutritional products, instrumental method for the analysis of vitamins Thomas SH;Shyamala S;Rebecca A
  16. J AOAC International v.72 Simultaneous liquid chromatographic determination water-soluble vitamins, caffeine, and preservative in oral liquid tonics Yumie M;Masayoshi Y;Kazuhiro O;Shiro S;Toshio M;Hiroyuki N
  17. J Chromatogr v.301 New ion-pair reagent for the high performance liquid chromatographic separation of B-group vitamins in pharmaceuticals Woollard DC https://doi.org/10.1016/S0021-9673(01)89222-0
  18. J Korean Soc Food Sci Nutr v.28 Determination of Vitamin $B_12$ in foods using column-switching technique in $\mu$-HPLC Park SJ;Kim HK;Hahm TS;Kim B
  19. Chromatogrphia v.30 Simutaneous determination of cefoxitin, cefuroxine, cephalexin and cepaoridirine in plasma using HPLC and column-switching technique Lee YJ;Lee HS https://doi.org/10.1007/BF02270453
  20. J Chromatogr v.424 Quantitative analysis of retinoids in biological fluids by high performance liquid chromatography using column switching. I. determination of isotretinoin and tretinoin and their 4-oxo metabolites in plasma Wyss R;Buchell F https://doi.org/10.1016/S0378-4347(00)81107-X
  21. Chromatographia v.44 Simultaneous determination of parathion and metabolites in serum by HPLC column switching Lee HS;Kim K;Kim JH;Do DS;Lee SK https://doi.org/10.1007/BF02466739
  22. J Chromatogr v.707 Column-switching high performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection Nielsen SE;Dtrasted LO https://doi.org/10.1016/S0378-4347(97)00574-4
  23. J Pharm Biomed Anal v.30 Analysis of an anti-inflammatory steroidal drug, difluprednate in aueous humor by combination of semi-micro HPLC and column switching method Yasueda S;Kimura M;Ohtori A;Kakehi K https://doi.org/10.1016/S0731-7085(02)00515-0
  24. J Chromatogr v.707 Column-switching high performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection Nielsen SE;Dtrasted LO https://doi.org/10.1016/S0378-4347(97)00574-4
  25. J Pharm Biomed Anal v.30 Analysis of an anti-inflammatory steroidal drug, difluprednate in aueous humor by combination of semi-micro HPLC and column switching method Yasueda S;Kimura M;Ohtori A;Kakehi K https://doi.org/10.1016/S0731-7085(02)00515-0

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