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Inhibition of Adventitious Root Growth in Boron-Deficient or Aluminum-Stressed Sunflower Cuttings

  • Published : 2003.11.01

Abstract

The effect of boron and aluminum on the development of adventitious roots was studied in sunflower cuttings. Three-day-old seedlings were de-rooted and grown in nutrient solutions with or without boron and supplemented with different concentrations (from 50 to 700 ${\mu}$M) of aluminum. The number and length of the adventitious roots and proline content in adventitious roots in response to insufficient boron and aluminum stress were determined periodically. The micronutrient boron caused the development of numerous roots in the lower parts of the hypocotyl. A dose-response of boron-induced rooting yielded an optimum concentration of 0.1 mM boron. In the absence of boron, in the majority of the adventitious roots, a significant inhibition was observed with or without aluminum, indicating that the most apparent symptom of boron deficiency is the cessation of root growth. Increasing concentrations of aluminum caused progressive inhibition of growth and rooting of the hypocotyls, and a parallel increase in proline levels of adventitious roots. Supplemental boron ameliorated the inhibitory effect of aluminum, suggesting that aluminum could inhibit root growth by inducing boron deficiency. Ascorbate added to medium in the absence of boron improved root growth and induced a significant decrease in proline levels. These findings suggest that adventitious root growth inhibition resulting from either boron deficiency or aluminum toxicity may be a result of impaired ascorbate metabolism.

References

  1. Annals of Botany v.37 The effect of boric acid and borax on the broad bean and certain other plants Warington,K.
  2. Mineral Nutritiion of Higher Plants, (2nd ed.) Marschner,H.
  3. Physiology and biochemistry of boron in plants;Boron and Its Role in Crop Production Shelp,B.J.;Gupta,U.C.(ed.)
  4. Plant Physiol. v.110 Two chains of rhamnogalacturonan Ⅱ are cross linked by borate-diol ester bonds in higher plant cell walls Kobayashi,M;T.Matoh;J.Azuma https://doi.org/10.1104/pp.59.6.1047
  5. Plant Physiol.59 Early effects of boron deficiency on indoleacetic acid oxidase levels ofj squash root tips Bohnsack,C.W.;L.S.Albert https://doi.org/10.1104/pp.110.3.1017
  6. Plant and Soil v.193 Boron deficiency induced impairments of cellular functiions in plants Cakmak,I.;V.Romheld https://doi.org/10.1023/A:1004259808322
  7. Plant and Soil v.193 boron in plant cell walls Matoh,T. https://doi.org/10.1023/A:1004207824251
  8. Biofactors v.3 Chemistry and biology of boron Loomis,W.D.;R.W.Durst
  9. Plant Physiol. v.105 Localization of boron in cell walls of squash and tobacco and its association with pectin Hu,H.;P.H.Brown https://doi.org/10.1104/pp.105.2.681
  10. Jour. Exp. Bot. v.295 Species variability in boron requirement is correlated with cell wall pectin Hu,H.;P.H.Brown;J.M.Labavitch
  11. Plant Cell Physiol. v.37 Ubiquity of a borate-rhamnogalacturonan Ⅱ complex with cell wall pectin Hu,H.;P.H.Brown;J.M.Labavitch https://doi.org/10.1093/oxfordjournals.pcp.a028992
  12. Plant Physiol. v.93 boron induces hyperpolarization of sunflower root cell membranes and increase membrane permeabillity to $K^+$ Schon,M.K.;A.Novacky;D.G.Blevins https://doi.org/10.1104/pp.93.2.566
  13. Biochem. Mol. Biol. Int. v.31 The effect of boron on plasma membrane electron transport and associated proton secretion by cultured carrot cells Barr,R.;M.Bottger;F.L.Crane
  14. Protoplasma v.133 Auxin-stimulated NADH oxidase (semidehydroascorbate reductase) of soybean plasme membrane : Role in acidification of cytoplasm Morr,D.J.;P.Navas;C.Penel;F.J.Castillo https://doi.org/10.1007/BF01304635
  15. Agronomie v.12 Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots Gaspar,T.;C.Kevers,J.F.Hausman;J.Y.Benthon;V.Ripetti https://doi.org/10.1051/agro:19921003
  16. New Phytologist v.108 Effects of auxin and boron on nucleic acid metabolism and cell division during adventitious root regeneration Ali,A.H.N.;B.C.Jorvis https://doi.org/10.1111/j.1469-8137.1988.tb04178.x
  17. New Root Fomation in Plants and Cuttings Endogenous control of adventitious rooting in nonwoody cuttings Jarvis,B.C.;Jackson,M.B.(ed.)
  18. Kor. Jour. Life Science v.12 Effect of boron on the development of adventitious roots in sunflower seedings Go,E.J.;J.H.Hong https://doi.org/10.5352/JLS.2002.12.6.786
  19. Plant Mol. Biol. v.49 Rapid determination of free proline for water stress studies Bates,L.S.;R.P.Waldren;I.B.Teare https://doi.org/10.1007/BF00018060
  20. Annu. Rev. Plant Physiol. Plant Mol. Biol. v.49 Boron in plant structure and function Blevins,D.G.;K.M.Lukaszewski https://doi.org/10.1146/annurev.arplant.49.1.481
  21. Boron in Soils and Plants The occurrence and correction ofj boron deficiency Shorrocks,V.M.;Dell,B.(ed.);P.H.Brown(ed.);W.Bell(ed.)
  22. J. Exp. Bot. v.44 Aluminum toxicity in roots: An investigation of spatial sensitivity and the role of the root cap Ryan,P.R.;J.M.DiTomaso;L.V.Kochian https://doi.org/10.1093/jxb/44.2.437
  23. Soil Sci. Plant Nutr. v.43 Aluminum inhibits growth and stability of cortical microtubules in wheat (Triticum aestivum) roots Sasaki,M.;Y.Yamamoto;H.Matsumoto https://doi.org/10.1080/00380768.1997.10414772
  24. Research Issues in Aluminum Toxicity Aluminum toxicity and resistance in plants Kochian,L.V.;D.L.Jones;Yokel,R.A.(ed.);M.S.Golub(ed.)
  25. Plant Physiol. v.118 Alterations in the cytoskeleton accompany aluminum-induced growth inhibition and morphological changes in primary roots of maize Blancaflor,E.B.;D.L.Jones;S.Gilroy https://doi.org/10.1104/pp.118.1.159
  26. Plant Physiol. v.112 Root growth inhibition in boron deficient or aluminum-stressed squash may be a result of impaired ascorbate metabolism Lukaszewski,K.M.;D.G.Blevins https://doi.org/10.1104/pp.112.3.1135
  27. Plant Cell Environ. v.19 Prevention of aluminum toxicity wity supplemental boron. Ⅱ. Stimulation of root growth in acidic, high aluminum subsoil LeNoble,M.E.;D.G.Blevins;R.J.Miles https://doi.org/10.1111/j.1365-3040.1996.tb00429.x
  28. Plant Cell Environ. v.19 Prevention of aluminum toxicity with supplemental boron. Ⅰ. Maintenance of root elongation and cellullar structure LeNoble,M.E.;D.G.Blevins;R.E.Sharp;B.G.Cumbie https://doi.org/10.1111/j.1365-3040.1996.tb00428.x
  29. Jour. Exp. Bot. v.43 Influence of L-ascorbic acid on post-anoxic growth and survival of chickpea seedlings(Cicer arietinum L.) Crawford,R.M.M.;B.WollenweberRatzer https://doi.org/10.1093/jxb/43.5.703
  30. J. Plant Physiol. v.147 Inhibition of etiolated lupin hypocotyl growth and rooting by peroxidases, ascorbate and glutathione Cano,A.;A.Artes;M.B.Amao;J.SanchezBravo;M.Acosta
  31. J. Exp. Bot. v.50 Proline synthesis and dehydration. A model system for elucidating stress signal transduction Hare,P.D.;W.A.Cress;J.Van Staden https://doi.org/10.1093/jexbot/50.333.413
  32. Plant Physiol. v.96 growth of the maize primary root at low water potentials. Ⅲ. Role of increased proline deposition in osmotic adjustment Voetberg,G.S;R.E.Sharp https://doi.org/10.1104/pp.96.4.1125
  33. Plant Physiol. v.119 Proline accumultion in maize(Zea mays L.) primary roots at low water potentials. Ⅱ. Metabolic source of increased proline deposition in the elongation zone Versiues,P.E.;R.E.Sharp https://doi.org/10.1104/pp.119.4.1349
  34. Plant Soil v.137 Organic acid and free proline accumulatiion and nitrate reductase activity in sorghum(Sorghum bicolor) genotypes differing in aluminum toerance Galvez,L.;R.B.Clark;L.A.Klepper;L.Hansen
  35. J. Plant Physiol. v.150 Aluminum and water stress effects on growth and proline of sorghum Zaifnejad,M.;R.B.Clark;C.Y.Sullivan https://doi.org/10.1016/S0176-1617(97)80130-7