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In Vitro Propagation of Anthurium andreanum ′Atlanta′ Developed for Pot Culture

분화용 Anthurium andreanum ′Atlanta′의 기내번식

  • Published : 2003.06.01

Abstract

In order to establish micropropagation system Anthurium andreanum 'Atlanta', dwarf type, shoots of A. andreanum were cultured on medium supplemented with cytokinin. Callus was formed from the base of shoots. high frequency callus induction was obtained on medium with 10.0mg/L BA or 10.0mg/L TDZ(thidiazuron) at more than 71.8%. The shoots were cultured on media with various combinations and concentrations of TDZ, BA and 2.4-D to enhance callus induction. Callus was induced at more than 72.6% and grew vigorously on media containing 10.0mg/L BA and 0.0∼0.5mg/L 2.4-D, or 1.0mg/L TDZ. Stimulation effects of cytokinin by 2.4-D did not occur in combined treatments of cytokinin and 2.4-D. Callus was cut into sections(7${\times}$10mm), and then cultured on media with BA alone or BA and 2.4-D to regenerate shoots and to stimulate the callus growth. Shoot regeneration and callus growth were effective on media with 10.0mg/L BA alone, or 10.0mg/L BA and 0.1mg/L 2.4-D. In combined treatments of BA and 2.4-D, stmulation effects of cytocinin by 2.4-D also did not occur. Callus growth was decreased, accordiong to increasing the concentration of 2.4-D. In cimbined treatments of TDZ and 2.4-D in shoot regeneration and callus proliferation, stimulated effects of cytokinin by 2.4-D did not occur entirely. Media with 0.5∼1.0mg/L TDZ ingibited the regeneration and rooting of shoots, and callus growth from callus sections. Addition of 2.4-D on medium with TDZ ingibited the regeneration and rooting of shoots, and callus growth. Rooted plantdts were acclimatized in greenhouse. The plantlets were survived more than 98% in soil of vermiculite alone or mixed perlite 1 and vermiculite 1.

Keywords

Callus induction;regeneration;TDZ(thidiazuron)

References

  1. Fellman CD, Read PE, Hosier MA (1987) Effect of thidiazuron and CPPU on meristem formation and shoot proliferation. HoriSci 22: 1197-1200
  2. Finnie JF, van Staden J (1986) In vitro culture of Anthurium andreanum. S Afr J Bot 52: 343-346
  3. Geier T (1986) Factors affecting plant regeneration from leaf segments of Anthurium schazaianum Schott (Araceae) cultured in vitro. Plant Cell Tiss Org Cult 6: 115-125
  4. Geier T (1990) Anthurium In: Ammirato Pv, Evans DR, Sharp WR, Bajaj YPS eds. Handbook of plant call culture. McGraw Hill Publishing Co NewYork, pp 228-252
  5. Han BH, Joung HY, Ko JY (1997) In vitro propagation of Ficus benjamina by shoot tip culture. J Kor Soc Hort Sci 38: 15-319
  6. Kuehnle AR, Sugii N (1991) Callus induction and plantlet regeneration in tissue culture of Hawaiian anthuriums. HortSci 26: 919-921
  7. Kunisaki JT (1980) In vitro propagation of Anthurium andreanum Lind. HortSci 15: 508-509
  8. Kusey WE, Hummer PA, Weiler TC (1980) In vitro propagation of Gypsophilapaniculata L 'Bristol Fairy'. HortSci 15: 600-601
  9. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  10. Pennazio S (1975) Effect of adenine and kinetin on development of carnation tips cultured in vitro. J Hort Sci 50: 161-164
  11. Pierik RLM (1975) Callus multiplication of Anthurium andreanum Lind. in liquid media. Neth J Agri Sci 23: 199-302
  12. Pierik RML, Steegmans HHM, van der Meys JAJ (1974) Plantlet formation in callus tissues Anthurium andreanum Lind. Sci Hortc 2: 193-198 https://doi.org/10.1016/0304-4238(74)90009-0
  13. Reynolds JF (1987) Chemical regulation in tissue culture: An overview. HortSci 22: 1192-1194
  14. Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation. Sym SocExp BioI 11: 118-131
  15. Takayama S, Misawa M (1982) Regulation of organ formation by cytokinin and auxin in Lilium bulbscales grown in vitro. Plant Cell Physiol 23: 67-74
  16. Teng WL (1997) Regeneration of Anthurium adventitious shoots using liquid or raft culture. Plant Cell Tiss Org Cult49: 153-156 https://doi.org/10.1023/A:1005845931345
  17. Visser C, Qureshi JA, Gill R, Saxena PK (1992) Morphoregulatory role of thidiazuroe; Substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures. Plant Physiol 99: 1704-1707 https://doi.org/10.1104/pp.99.4.1704
  18. Yu KJ, Peak KY (1995a) Micropropagation of Anthurium spp. through shoot tip and callus culture. J Kor Soc Hort Sci 36: 684-694
  19. Yu KJ, Paek KY (1995b) Effect of macroelement levels in the media on shoot tip culture of Anthurium spp. and reestablishment of plantlets in soil. J Kor Soc Hort Sci 36: 893-899

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