Effects of Field Application of Spodoptera litura Nucleopolyhedrovirus to Control S. litura in Chrysanthemum

국화 포장에서 담배거세미나방 방제를 위한 핵다각체병바이러스 살포효과

  • 김선곤 (전남농업기술원 식물환경연구과) ;
  • 박종대 (전남농업기술원 식물환경연구과) ;
  • 김도익 (전남농업기술원 식물환경연구과) ;
  • 임대준 (농업과학기술원 농업해충과) ;
  • 김규진 (전남대학교 농생물학과) ;
  • 유용만 (㈜경농중앙연구소)
  • Published : 2003.06.01


This experiment was conducted to investigate control effect of Spodoptera litura using nucleopolyhedrovirus in chrysanthemum seedlings of open field and plastic house. Values of LT$_{50}$ of treatment 1.0$\times$10$^{6.8}$ PIBs/ml were 6.2-5.1 days in open field and 6.9-5.4 days in plastic house. Values of LT$_{50}$ and LT$_{95}$ were shorter in open field than in plastic house. Cumulative mortality was 100% in 1.0$\times$10$^{7.8}$ PIBs/ml and also higher in open field than in plastic house. In chrysanthemum field, organic synthetic insecticide (endosulfan EC) killed S. litura larvae in 2 days after application. Motality of S. litura larva inoculated with S. litura nucleopolyhedrovirus (SINPV) 1.0$\times$10$^{6.8}$ PIBs/ml was found out from 4 days after application and maintained during 14 days. Protection values of with SINPV 1.0$\times$10$^{8}$ PIBs/ml after 16 days were 94.0% and 89.4% in open field and plastic house, respectively, and those of endosulfan 350 ppm were 91.4% and 88.6%, respectively.


Tabacco cutworm;Spodoprera litura;Nucleopolyhedrovirus;Chrysanthemum;Field application;Mortality


  1. Im, D.J., K.M. Choi, M.H. Lee, B.R. Jin and S.K. Kang. 1989. In vitro mass production of Spodoptera litura nuclear polyhedrosis virus. Korean J. Appl. Etomol. 28: 82-87
  2. Jiang, J.X., G.W. Liang, Z.X. Jiang and G.W. Liang. 1999. Efficacy of a nuclear polyhedrosis virus for control of an experimental population of Spodoptera litura. Natural Enemies of Insects. 21: 13-17
  3. Kim, C.H. and H.Y. Shin. 1987. Studies on bionomics and control of tabacco cutworm, Spodoptera litura Fabricius in southern part of Korea. J. Insect. Sci. 5: 33-36
  4. Rao G.V., J.A. Wightman and D.V. Ranga Rao. 1989. Threshold temperatures and thermal requirements for the development of Spodoptera litura (Lepidoptera: Noctuidae). Environ. Entomol. 18: 548-551 https://doi.org/10.1093/ee/18.4.548
  5. Willink, E., M.Y. Romero, V.M. Osores, J.C. Ramallo and M.Y. De-Romero. 1995. Baculovirus for the biological control of the maize worm. Advance-Agroindustrial. 16: 18-23
  6. Im, D.J., B.S. Park, B.R. Jin, K.M. Choi and S.K. Kang. 1988 Pathogencity of nuclear polyhedrosis virus isolated from the tobacco cutworm, Spodoptera litura. Korean. J. Appl. Entomol. 27:219-224
  7. SAS Institute. 1991. SAS/STAT user's guide: Statistics, version 6.04. SAS Institute, Cary, N.C
  8. Im, D.J., B.R. Jin, K.M. Choi and S.K. Kang. 1990. Microbial control of the tobacco cutworm, Spodoptera litura (Fab.), using S. litura nuclear polyhedrosis virus. III. Field evaluation of the viral inseciticides. Korean J. Appl. Entomol. 29: 252-256
  9. Jaques, R.P. 1977. Stability of entomopathogenic viruses. Misc. Publ. Entomol. Soc. Am. 10:99-116
  10. Oliver, A.D. 1964. Studies on the biological control of the fall wedworm, Hyphantria cunea, in Louisiana. J. Econ. Entomol 57: 314-318 https://doi.org/10.1093/jee/57.3.314
  11. Geissler, K. 1995. Application and importance of entomopathogenie viruses with respect to integrated pest management. Archives of Phytopathology and Plant Protection 29: 479-483
  12. Bae, S.D. and H.J. Cho. 1998. Study on the physio-biology and control of Spodoptera Iitura Fabricius. Pl. Environ. Res. Rept. Nat. Yeongnam Agric. Expt. Sta. RDA. pp. 877-907
  13. Finney, D.J. 1971. Probit analysis 3rd ed., 333pp. Cambridge Univ. Press, Cambridge
  14. Kurstak, E. 1982. In microbial and viral pesticides, pp. 335-507. Dekker. New York
  15. Moawad, G.M., G.B. El-Saadany and S. Tantawy. 1992. Transmission of nuclear polyhedrosis virus to larval instars of the cotton leafworm Spodoptera littoralis Boisd. Egyptian J. Agri. Res. 70: 87-96