Inhibition of Listeria monocytogenes in Vacuum or Modified Atmosphere-Packed Ground Beef by Lactococcal Bacteriocins

  • 발행 : 2003.06.01


We investigated the antagonistic effects of two lactococcal bacteriocins, nisin or lacticin NK24, on the growth and the survival of Listeria monocytogenes in vacuum or modified atmosphere-packaged ground beef, Ground beef was inoculated with approximately 3 log colony-forming units (CFU) of L. monocytogenes ATCC 15313 culture per gram of ground beef. Inoculated samples were blended with/without 100 AU/g nisin or lacticin NK24, and subsequently vacuum or modified atmosphere packed at 4$^{\circ}C$. Listeria in the bacteriocin-treated and control samples was subsequently isolated from both vacuum and modified atmosphere packs and enumerated as CFU on Listeria Isolation Agar medium. Microbial counts in ground beef treated with bacteriocin declined steadily, while those of non-treated beef samples increased steadily. The results obtained demonstrate that nisin inhibits the growth of L. monocytogenes more effectively than lacticin NK24 at 100 AU/g. The use of lactococcal bacteriocins, such as nisin or lacticin NK24, in vacuum or modified atmosphere packaging offers a promising approach for eliminating or reducing the risk of L monocytogenes contamination in ground beef.


  1. Laukova A, Czikkova S, Dobransky T, Burdova O. 1999. Inhibition of Listeria monocytogenes and Staphylococcus aureus by enterocin CCM 4231 in milk products. Food Micobiol 16: 93-99
  2. Vignolo G, Fadda S, de Kairuz MN, de R Holgado AP, Oliver G. 1998. Effects of curing additives on the control of Listeria monocytogenes by lactocin 705 in meat slurry. Food Microbiol 15: 259-264
  3. Boutterfroy A, Linder M, Miilliere JB. 2000. Predictive models of the combined effects of curvaticin 13, NaCl and pH on the behaviour of Listeria monocytogenes ATCC 15313 in broth. J Appl Microbiol 88: 919-929
  4. Castellano P, Farias ME, Holzapfel W, Vignolo G. 2001. Sensitivity variations of Listeria strains to the bacteriocins, lactocin 705, enterocin CRL35 and nisin. Biotechnol Lett 23: 605-608
  5. Duffes F, Jenoe P, Boyaval P. 2000. Use of two-dimensional electrophoresis to study differential protein expression in divercin V41-resistant and wild-type strains of Listeria monocytogenes. Appl Environ Microbiol 66: 4318-4324
  6. Grahame WG. 1996. Methods for preservation and extension of shelf life. Int J Food Microbiol 33: 51-64
  7. Ennahar S, Deschamps N. 2000. Anti-Listeria effect of enterodin A, produced by cheese-isolated Enterococcus faecium EFMO1, relative to other bacteriocins from lactic acid bacteria. J Appl Microbiol 88: 449-457
  8. Francis GA, David O. 1998. Effects of storage atmosphere on Listeria monocytogenes and competing microflora using a surface model system. Int J Food Sci Technol 33: 465-476
  9. Khalil E, Jamal EY, Badr-Din R. 2000. Synergistic effects of nisin and thymol on antimicrobial activities in Listeria monocytogenes and Bacillus subtilis. FEMS Microbiol Lett 183: 191-195
  10. Alpas H, Bozoglu F. 2000. The effect of high hydrostatic pressure, heat and bacteriocins on inactivation of foodborne pathogens in milk and orange juice. World J Microbiol Biotechnol 16: 387-392
  11. Ming X, Weber GH, Ayres W, Sandine WE. 1997. Bacteriocins applied to food packaging materials to inhibit Listeria monocytogenes on meats. J Food Sci 62: 413-415
  12. Xitain M, George HW, James WA, William ES. 1997. Bacteriocins applied to food packaging materials to inhibit Listeria monocytogenes on meats. J Food Sci 62: 413-415
  13. Lee N-K, Paik H-D. 2001. Partial characterization of lacticin NK24, a newly identified bacteriocin of Lactococcus lactis NK24 isolated from Jeot-gal. Food Micobiol 18: 17-24
  14. Aymerich T, Artigas MG, Garriga M, Monfort JM, Hugas M. 2000. Effect of sausage ingredients and additives on the production of enterocins A and B by Enterococcus faecium CTC492. Optimization of in vitro production and antilisterial effect in dry fermented sausages. J Appl Microbiol 88: 686-694
  15. Chung KT, Dickson JS, Crouse JD. 1989. Effects of nisin on growth of bacteria attached to meat. Appl Environ Microbiol 55: 1329-1333
  16. Nielsen JW, Dickson JS, Crouse JD. 1990. Use of a bacteriocin produced by Pediococcus acidilactici to inhibit Listeria monocytogenes associated with fresh meat. Appl Environ Microbiol 56: 2142-2145
  17. Hugas M, Pages F, Garriga M, Monfort JM. 1998. Application of the bacteriocinogenic Lactobacillus sakei CTC494 to prevent growth of Listeria in fresh and cooked meat products packed with different atmospheres. Food Micobiol 15: 639-650
  18. Morgan SM, Ross RP, Beresford T, Hill C. 2000. Combination of hydrostatic pressure and lacticin 3147 causes increased killing of Staphylococcus and Listeria. J Appl Microbiol 88: 414-420

피인용 문헌

  1. Perspectives for the Industrial Use of Bacteriocin in Dairy and Meat Industry vol.28, pp.1, 2008,