Dimerization of Thyroglobulin in the Endoplasmic Reticulum

Thyroglobulin의 소포체내 dimerization

  • Published : 2003.06.01


The kinetics of dimerization of a newly synthesized thyroglobulin (Tg), the precursor protein in the manufacture of thyroid hormone, was investigated in the endoplasmic reticulum of thyrocytes FRTL-5 cell line. The folded monomeric Tg was first detectable in a conformationally unstable form, from the examination of lysates of pulse labeled cultured thyrocytes by denaturing and nondenaturing gel electrophoresis by 15 min after biosynthesis. The first dimeric Tg was formed by 30 min after; the monomer declined and the dimer progressively increased, and 40 min after remarkable dimeric Tg form was found. Finally, dimerization was complete at 60 min after.


  1. Arvan, P. and J. Lee. 1991. Regulated and constitutive protein targeting can be distinguished by secretory polarity in thyroid epithelial cells. J. Cell Bioi. 112, 365-376
  2. Baudry, N., P. J. Lejeune., P. Niccoli., L. Vinet., P. Carayon and B. Mallet. 1996. Dityrosine bridge for-mation and thyroid hormone synthesis are tightly linked and are both dependent on N-glycans. FEBS Lett. 396, 223-226
  3. Deshpande, V. and S. G. Venkatesh. 1999. Thyro-globulin, the prothyroid hormone: chemistry, synthesis and degradation. Biochim. Biophys. Acta. 1430, 157-178
  4. Di Jesc, B., R. Pereira., E. Consiglio., S. Formisano., J. Satrustegui and I. V. Sandoval. 1998. Demonstration of a $Ca^{2+}$ requirement for thyroglobulin dimerization and export to the golgi complex. Eur. J. Biochem. 252, 583-590
  5. Fewell, S. W., K. J. Travers., J. S. Weissman and J. L. Brodsky. The action of molecular chaperones in the early secretory pathway. 2001. Ann. Rev. Genet. 35, 149-191
  6. Freudl, R., M. Klose and U. Henning. 1990. Export and sorting of the Escherichia coli outer membrane protein OmpA. J. Bioenerg. Biomembr. 22, 441-449
  7. Kim, P. S. and P. Arvan. 1991. Folding and assembly of newly synthesized thyroglobulin occurs in a pre-Golgi compartment. J. Biol. Chem. 266, 12412-12418
  8. Kim, P. S., D. Bole and P. Arvan. 1992. Transient aggregation of nascent thyroglobulin in the endoplasmic reticulum: relationship to the molecular chaperone, BiP. J. Cell BioI. 118, 541-549
  9. Kim, P. S., O. Y. Kwon and P. Arvan. 1996. An en-doplasmic reticulum storage disease causing congenital goiter with hypothyroidism. J. Cell Bioi. 133, 517-527
  10. Petaja-Repo, U. E., M. Hogue., A. Laperriere., P. Walk-er and M. Bouvier. 2000. Export from the endoplasmic reticulum represents the limiting step in the maturation and cell surface expression of the human delta opioid receptor. J. Biol. Chem. 275, 13727-13736