Identification of Prevotella intermedia ATCC 25611 Using Pi29-L DNA Probe.

Pi29-L DNA 프로브를 이용한 Prevotella intermedia ATCC 25611의 동정

  • 국중기 (조선대학교 치과대학 구강생화학 교실) ;
  • 백동헌 (단국대학교 치과대학 미생물 및 면역학 교실)
  • Published : 2003.06.01

Abstract

Recently, we introduced a new method for rapid screening of bacterial species- or subspecies-specific DNA probes, named “inverted dot blot hybridization screening method”. We then applied this method to develop species- or strain- specific DNA probes for Prevotella intermedia and Prevotella nigrescens. In those studies, among 96 candidate DNA probes which were screened by the new method, 5 probes were confirmed as being putatively strain-specific : 3 probes for P. nigrescens 9336 (ATCC 33563), one for each p. intermedia ATCC 25611 and one for P. nigrescens G8-9K-3 (ATCC 49046). In the present study, we evaluated by Southern blot analysis a DNA probe Pi29-L, one of the 96 candidate probes described above, whether it is specific for the strain ATCC 25611 off. intermedia. Our data show that the probe Pi29-L is potentially P. intermedia ATCC 25611-specific, which can be useful for the detection and identification of the strain, particularly in maintenance of the strain.

Keywords

DNA probe;identification;Pi29-L;Prevotella intermedia ATCC 25611

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