PCR Detection of Terephthalic Acid Degrading Comamonas testosteroni in Soil

PCR을 이용한 토양 중 Terephthalic Acid 분해 Comamonas testosteroni의 검출

  • 이종훈 (경기대학교 식품생물공학과) ;
  • 강동주 (경기대학교 식품생물공학과) ;
  • 홍연표 (중앙대학교 예방의학교실)
  • Published : 2003.06.01

Abstract

Eleven bacterial strains which are able to utilize terephthalic acid as a carbon and an energy source for growth were isolated from the soil of 7 water quality evaluation points in Kyonggi area of Korea. Phthalic acid isomer degrading activity of the isolates from the 4 contaminated points was higher than those from the 3 clean points. Among 11 isolates, 4 isolates which have high terephthalic acid degrading activity and degrade two phthalic acid isomers were identified by partal 16S rDNA sequence determination. One of them was identified as Pseudomonas putida, and the others as Comamonas testosteroni. Thus a large number of phthalic acid isomer degrading bacteria in domestic soil were inferred as C. testosteroni. On the basis of these results, the PCR detection of C. testosteroni in soil was applied to monitor soil contamination by phthalic acid isomers. The DNA of C. test-osteroni extracted from 4 g soil was directly detected by PCR with C. testosteroni specific primer pair. The amount of PCR products was different according to sampling sites and more PCR products were obtained from contaminated sites than those from clean sites (Gulpo-chun>Anyang-chun>Hwangguji-chun>Shin-chun>Huk-chun>Pukhan-river>Kapyeong-chun). This result was coincided with that of the viable cell counts for terephthalic acid degrading bacteria.

Keywords

Terephthalic acid;phthalic acid isomer;Comamonas testosteroni;PCR detection

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