Characterization of the \beta-Galactosidase Produced by Streptomyces sp. YB-10

\beta-Galactosidase를 생산하는 Streptomyces sp. YB-10의 분리 및 효소 특성

  • 윤기홍 (우송대학교 식품생명과학부) ;
  • 이경섭 (우송대학교 식품생명과학부) ;
  • 김창진 (한국생명공학연구원)
  • Published : 2003.06.01

Abstract

A strain YB-10 was isolated from soil as a producer of the extracellular $\beta$-D-galactosidase, which catalyzes the hydrolysis of lactose. The strain YB-10 was identified as Streptomyces sp. on the basis of its cultural, morphological and physiological properties. After treating culture supernatant of the isolate with ammonium sulfate, the precipitated protein was used as a crude $\beta$-galactosidase for analyzing its reaction properties with para-nitrophenyl-$\beta$-D-galactosidase(pNP-$\beta$Gal) as a substrate. The $\beta$-galactosidase showed its maximal activity at pH 6.0 and 6$0^{\circ}C$. The enzyme was also active on lactose. The hydrolyzing activity of $\beta$-galactosldase for pNP-$\beta$Gal and lactose was decreased by galactose. Its hydrolyzing activity far lactose was also decreased by glucose, but the activity for pNP-$\beta$Gal was increased to 1.8-folds by glucose.

Keywords

Streptomyces;identification;\beta-galactosidase;property;reaction product

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