Characterization of the \beta-Galactosidase Produced by Streptomyces sp. YB-10

\beta-Galactosidase를 생산하는 Streptomyces sp. YB-10의 분리 및 효소 특성

  • 윤기홍 (우송대학교 식품생명과학부) ;
  • 이경섭 (우송대학교 식품생명과학부) ;
  • 김창진 (한국생명공학연구원)
  • Published : 2003.06.01


A strain YB-10 was isolated from soil as a producer of the extracellular $\beta$-D-galactosidase, which catalyzes the hydrolysis of lactose. The strain YB-10 was identified as Streptomyces sp. on the basis of its cultural, morphological and physiological properties. After treating culture supernatant of the isolate with ammonium sulfate, the precipitated protein was used as a crude $\beta$-galactosidase for analyzing its reaction properties with para-nitrophenyl-$\beta$-D-galactosidase(pNP-$\beta$Gal) as a substrate. The $\beta$-galactosidase showed its maximal activity at pH 6.0 and 6$0^{\circ}C$. The enzyme was also active on lactose. The hydrolyzing activity of $\beta$-galactosldase for pNP-$\beta$Gal and lactose was decreased by galactose. Its hydrolyzing activity far lactose was also decreased by glucose, but the activity for pNP-$\beta$Gal was increased to 1.8-folds by glucose.


Streptomyces;identification;\beta-galactosidase;property;reaction product


  1. Appl. Environ. Microbiol. v.61 Purification and properties of a novel thermostable galacto-oligosaccharide-producing beta-galactosidase from Sterigmatomyces elviae CBS8119 Onishi,N.;T.Tanaka
  2. Mol. Biotechnol. v.10 Cloning and nucleotide sequence of the thermostable beta-galac-tosidase gene from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme Dabrowski,S.;J.Maciunska;J.Synowiecki
  3. Acta Microbiol. Pol. v.50 Optimization of α-galactosidase production in Streptomyces erythrus Elshafei,A.M.;M.S.Foda;E.Abdel-Mobde;N.H.Ali
  4. Eur, J. Biochem. v.187 Thermostable beta-galactosidase from the archaebacterium Sulfolobus sol-fataricus Pisani,F.M.;R.Rella;C.A.Raia;C.Rozzo;R.Nucci;A.Gambacorta;M.Rosa;M.Rossi
  5. Bergey's Mannual of Systematic Bacteriology v.4 Suprageneric classification of Actinomyces Goodfellow,M.;T.Cross;H.A.Lechevalier;S.T.Wiliams(ed.);M.E.Sharpe(ed.);J.G.Holt(ed.)
  6. J. Dariy. Sci. v.83 Use of chemical mutagenesis for the isolation of food grade beta-galactosidase overproducing mutants of bifidobacteria, lactobacilli and Streptococcus thermophilus Ibrahim,S.A.;D.J.O'Sullivan
  7. J. Gen. Microbiol. v.129 Numerical classification of Streptomyces and related genera Williams,S.T.;M.Goodfellow;G.Alderson;E.M.H.Wellington;P.H.A.Sneath;M.Sackin
  8. Kor. J. Microbiol. Biotechnol. v.30 Characterization of extracellular α-galactrosidase produced by Streptomyces sp. YB-4 Kim.S.Y.;K.H.Cho;C.J.Kim;D.J.Park;K.H.Yoon
  9. J Milk Food Technol. v.34 Characterization of β-galactodisase from Saccharomyces fragilis Wendorff,W.L.;C.H.Amundson
  10. Appl. Environ. Microbiol. v.66 Characterization of a novel beta-galac-tosidase from Bifidobacterium adolescentis DSM 20083 active towards transgalactooligosaccharides van Laere,K.M.;T.Abee;H.A.Schols;G.Beldman;A.G.Voragen
  11. Appl. Microbiol. Biotechnol. v.40 Purification and properties of recombinant beta-galactosidase of the hyperthermophilic becterium Thermotoga maritima Gabelsberger,J.;W.Liebl;H.Schleifer
  12. Appl. Microbiol. Biotechnol. v.46 The effect of cations on the hydrolysis of lactose and the transferase reactions catalysed by beta-galactosidase from six strains of lactic acid bacteria Garman,J.;T.Coolbear;J.Smart
  13. Int. J. Syst. Bacteriol. v.16 Methods for the characterization of Streptomyces species Shirling,E.B.;D.Gottlieb
  14. Glycoconj. J. v.15 Purification and properties of recombinant beta-galac-tosidase from Bacillus circulans Fujimoto,H.;M.Miyasato;Y.Ito;T.Sasaki;K.Ajisaka
  15. Appl. Microbiol. Biotechnol. v.40 Purification and characterization of a thermostable beta-galactosidase with high transgalactosylation activity from Saccharopolyspora recitivirgula Nakao,M.;M.Harada;Y.Kodama;T.Nakayama;Y.Shibano;T.Amachi
  16. Carbohydr. Res. v.305 Regioselectivity of the enzymatic transgalactosidation of D-and L-xylose catalysed by beta-galactosidases Montero,E.;J.Alonso;F.J.Canada;A.Fernandez-Mayor-alas;M.Martin-Lornas
  17. Nahrung. v.41 Effect of bacterial galactosidase treatment on the nutritional status of soybean seeds and its milk derivative Sanni,A.I.;A.A.Onilude;O.R.Ogundoye
  18. Appl. Environ. Microbiol. v.67 Molecular and biochemical analysis of two beta-galactosidases from Bifidobacterium imfantis HL96 Hung,M.N.;Z.Xia;N.T.Hu;B.H.Lee
  19. J. Dairy Res. v.60 Growth of lactic acid bacteria and bifidobacteria on correlation with distribution of beta-galactosidase and phospho-beta-galactosi-dase Smart,J.B.;C.J.Pillidge;J.H.Garman
  20. Appl. Microbiol. Biotechnol. v.29 A thermostable beta-glucosidase isolated from a bacterial species the genus Thermus Takase,M.;K.Horikoshi
  21. Carbohydr. Res. v.329 Purification and characterisation of a beta-galactosidase from Aspergillus aculeatus with activity towards (modified) expolysaccharides from Lactococcus lactis subsp. cremoris B39 and B891 van Casteren,W.H.;M.Eimermann;L.A. van den Broek;J.P.Vincken;H.A.Schols;A.G.Voragen