Production of Enantioselective Lipase from Acinetobacter sp. SY-01

Acinetobacter sp. SY-01로부터 Enantioselective Lipase의 생산

  • 박대원 (한국과학기술연구원 수질환경 및 복원연구센터) ;
  • 박호일 (한국과학기술연구원 수질환경 및 복원연구센터) ;
  • 신평균 (한국과학기술연구원 수질환경 및 복원연구센터)
  • Published : 2003.06.01

Abstract

Lipase from Acinetobacter sp. SY-01 plays an important role enzyme that products chiral drug. We investigated optimum condition for mass production of Acinetobacter sp. SY-01 lipase. Addition of among the different oils to medium. olive oil was optimal for enzyme production. When 0.2% olive oil was added as a carbon source, the production of lipase was increased to a maximum. The optimum pH and temperature were pH 7 and $30^{\circ}C$. In the presence of $Fe^{2+}$ and $Ca^{2+}$, the lipase activity was dramatically enhanced by 280% and 160%, respectively. SY-01 lipase was stable in the most of the DMSO among organic solvents. The addition of triton-X 100 increased the SY-01 lipase by 100-fold. The optimum composition of medium for production of the enzyme was 0.8% yeast extract, 0.2% olive oil, 0.4% triton X-100+40% DMSO. 0.1% $NH_4Cl$, 0.4% $K_2HPO_4$ 3.9% $NaH_2PO_4$, 0.03% $CaCl_22H_2O$, 0.01% $FeSO_4$$7H_2O$(pH 7.0).

Keywords

Acinetobacter sp. SY-01;enantioselective lipase;itraconazole

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