Screening and Characterization of Probiotic Strains for Prevention of Bacterial Fish Diseases

어류의 세균성 질병 예방을 위한 Probiotic균주의 선발 및 특성

  • 허문수 (제주대학교 해양생산과학부) ;
  • 양병규 (제주대학교 해양생산과학부) ;
  • 전유진 (제주대학교 해양생산과학부)
  • Published : 2003.06.01

Abstract

The purpose of the present study was to screen the effective of lactic acid bacteria (LAB) as probiotics, which are able to protect aquacultural fish pathogenic bacteria, and investigate their characterization. Twenty strains of lactic acid bacteria were isolated from fish intestine, fermented fish foods and kimchis. These bacteria were screened for antagonistic activity against fish pathogenic bacteria. Seven tested LAB strains were able to inhibit the fish pathogenic bacteria, including Vibrio anguillarum, Edwardsiella tarda, and Streptococcus sp.. Of the probiotic candidates, BK19 strain isolated from fermented pollack viscera indicated the largest inhibition activity. Moreover, this strain showed a resistance over low pH and antibiotic agents. Therefore this probiotic candidate BK19 was finally selected and identified as a probiotic strain. This particular probiotic bacteria was identified as Lactobacillus sakei BK19 by biochemical characteristics and 165 rRNA PCR amplification.

Keywords

Fish diseases;probiotic;lactic acid bacteria;Vibrio anguillarum

References

  1. Food Sci. Technol. v.10 Probiotics: how should they be defined Trends Salminen,S.;A.C.Ouwehand;Y.Benno;Y.K.Lee
  2. Int. J. Food Microbiol. v.40 Plantaricin LP84, a broad spectrum heat-stable bactriocin of L. plantarum NCIM 2084 produced in a simple glucose broth medium Suma,K.;M.C.Misra;M.C.Varadaraj
  3. 2nd Asian Fish v.22 Spread of drug resistant strains of Streptococcus sp. in yellowtail farms Aoki.T.;K.Tamaki;T.Kitao
  4. Appl. Environ. Microbiol. v.58 Intestinal colonization potential of turbot Scophthalmus maximus and dab Limanda limanda-associated bacteria with inhibitory effects against Vibrio anguillarum Olsson,J.C.;A.Westerdahl;P.L.Conway,;S.Kjelleberg
  5. Annu. Rev. Fish Dis. v.4 Bacterial resistance to antimicrobial agents used in fish farming: a critical evaluation of method and meaning Smith,P.;M.P.Hiney;O.B.Samuelsen
  6. Am. J. Clin. Nutr. v.69 Probiotics prebiotics, and synbiotics: approaches for modulating the microbial ecology of the gut Collins,M.D.;G.R.Gibson
  7. Appl. Environ. Microbiol. v.55 Antibacterial activity of Lactobacillus sakei isolated from meat Schillinger,U.;F.K.Lucke
  8. Infection v.27 Selective pressure by antibiotics as feed additived Witte,W.;I.Klare;G.Wernet
  9. Vaccine v.13 Vaccination with whole cell vaccine and bacterial protein extract protects tilapia against Streptococcus difficile meningo encepalitis Eldar,A.;O.Shapiro;Y.Bejerano;H.Bercovier
  10. Microbiol. Mole. Biol. Rev v.64 probiotic bacteria as biological control agents in aquaculture Verschuere,L.;G.Rombaut;P.Sorgeloos;W.Verstraete
  11. Food Microbiol. v.13 Antimicrobial activity of sourdough lactic acid bacteria: isolation of a bacteriocinlike inhibitory substance from Lactobacillus san-francisco C57 Corsetti,A.;M.Gobbetti;E.Smacchi
  12. Aquaculture v.160 Lactic acid bacteria in fish: a review Ringo,E.;F.J.Gatesoupe
  13. Lait. v.72 Inhibiting factors produced by lactic acid bacteria. 2. Bacteriocins and other antibacterial substances Piard,J.C.;M.Desmazeaud
  14. J. Gen. Microbiol. v.138 Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sakei LB 706 Holck,A.;L.Axellson;S.E.Birkeland;T.Aukrust;H.Bloom
  15. J. Appl. Bacteriol. v.66 Probiotics in man and animal Fuller,R.
  16. Appl. Environ. Microbiol. v.63 Antagonistic activity exerted in vitro and in vivo by Lactobacillus casei (strain GG) against Salmonella typhimurium C5 infection Hudault,S.;V.Lievin;M.F.Bernet-Camard;A.L.Servin
  17. J. Appl. Microbiol. v.86 Partial characterization and cloning of leuconocin J, a bacteriocin produced by Leuconostoc sp. J2 isolated from the Korean fermented vegetable Kimchi Choi,H.J.;H.S.Lee;S.Her;D.H.Oh;S.S.Yoon