DOI QR코드

DOI QR Code

Analysis of the Interactive Characteristic of Environmental Toxic Peptide and Phospholipid

환경 독성 Peptide의 인지질과의 상호 작용 특성 분석

  • 이봉헌 (부산대학교 화학과) ;
  • 박흥재 (인제대학교 환경시스템학부)
  • Published : 2003.01.01

Abstract

The interaction of mastoparan B, a cationic tetradecapeptide amide isolated from the hornet Vespa basalis, with phospholipid bilayers was studied with synthetic mastoparan B and its analogue with Ala instead of hydrophobic 12th amino acid residue in mastoparan B. MP-B and its derivative, [12-Ala]MP-B were synthesized by the solid-phase peptide synthesis method. MP-B and its analogue, [12-Ala]MP-B adopted an unordered structure in buffer solution. In the presence of neutral and acidic liposomes, the peptides took an $\alpha$-helical structure. The two peptides interacted with neutral and acidic lipid bilayers. These results indicated that the hydrophobic face in the amphipathic $\alpha$-helix of MP-B critically affected the biological activity and helical content.

Keywords

Interaction;Toxic peptide;Liposome

References

  1. Wang, C. Y. and L. Huang, 1984, Biochemistry, 23, 4409-4411. https://doi.org/10.1021/bi00314a026
  2. Barnette, M. S., R. Daly, and B. Weiss, 1983, BioChem. Phannacol., 32, 2929-2934. https://doi.org/10.1016/0006-2952(83)90398-2
  3. Higashijima, S., S. Uzu, T. Nakajima, and E. M. Ross, 1988, Biol. Commun., 263, 6491-6497.
  4. Uster, P. S. and D. W. Dreamer, 1985, Biochemistry, 24, 1-8. https://doi.org/10.1021/bi00322a001
  5. Park, N. G., Y. Yamato, S. Lee, G. Sugihara, J. S. Park, and S. W. Kang, 1996, Bull. Korean Chem. Soc., 17, 239-246.
  6. Gad, A. E., B. L. Silver, and G. D. Eytan, 1982, Biochirn. Biophys. Acta, 690, 124-128. https://doi.org/10.1016/0005-2736(82)90246-2
  7. Argiolas, A. and J. J. Pisano, 1983, Biol. Chem., 258, 13697-13702.
  8. Ho, C. L. and L. L. Hwang, 1991, BioChem. J., 274, 453-457. https://doi.org/10.1042/bj2740453
  9. Surewics, R. M., R. M. Epand, W. J. Vail, and M. A. Moscarelo, 1985, Biochim. Biophys. Acta, 820, 310-306.
  10. Okmura, K., K. Inui, Y. Hirai, and T. Nakajima, 1981, Biomed. Res., 2, 450-457. https://doi.org/10.2220/biomedres.2.450