Analysis of the Interactive Characteristic of Environmental Toxic Peptide and Phospholipid

환경 독성 Peptide의 인지질과의 상호 작용 특성 분석

  • 이봉헌 (부산대학교 화학과) ;
  • 박흥재 (인제대학교 환경시스템학부)
  • Published : 2003.01.01


The interaction of mastoparan B, a cationic tetradecapeptide amide isolated from the hornet Vespa basalis, with phospholipid bilayers was studied with synthetic mastoparan B and its analogue with Ala instead of hydrophobic 12th amino acid residue in mastoparan B. MP-B and its derivative, [12-Ala]MP-B were synthesized by the solid-phase peptide synthesis method. MP-B and its analogue, [12-Ala]MP-B adopted an unordered structure in buffer solution. In the presence of neutral and acidic liposomes, the peptides took an $\alpha$-helical structure. The two peptides interacted with neutral and acidic lipid bilayers. These results indicated that the hydrophobic face in the amphipathic $\alpha$-helix of MP-B critically affected the biological activity and helical content.


Interaction;Toxic peptide;Liposome


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