Application of Phytase, Microbial or Plant Origin, to Reduce Phosphorus Excretion in Poultry Production

  • Paik, InKee (Department of Animal Science, Chung-Ang University)
  • Published : 2003.01.01


In order to prevent pollution from animal waste, the excretion of nutrients should be reduced through proper nutritional management. Among the many nutrients of concern, such as N, P, Cu, Zn and K, P is one of the most concerned nutrients to be managed. Seven feeding trials, three with layers and four with broilers, were conducted to determine if microbial phytase supplementation can reduce non-phytate phosphorus (NPP) level in diets and results in concomitant reductions of P excretion. The results showed that microbial phytase can be successfully used to achieve these purposes. Activity of natural phytase in certain plant feedstuffs is high enough to be considered in feed formulation. Three experiments have been conducted to study the characteristics of plant phytase and its application to feeding of broilers. Selected brands of wheat bran could be successfully used as a source of phytase in broiler feeding.


  1. Kessler, J. and K. Egli. 1991. Phosphor sparen dank phytase: Erste Ergebnisse beim Mastschwein. Landwirtschaft Schweiz Bd. 5:5-9.
  2. Kim, B. H., H. Namkung and I. K. Paik. 2002. Utilization of plant phytase to improve phosphorus availability. Kor. J. Anim. Sci. 44(4):407-418.
  3. Lee, S. J., J. S. Um, H. Namkung and I. K. Paik. 1999. Studies on the content of phytase phosphorus and the characteristics of phytase activity in cereals and cereal by-products. Kor. J. Anim. Nutr. Feed 23(6):501-506.
  4. Lee, S. J., J. S. Um, I. K. Paik and J. G. Lee. 2000. Effect of Crude Phytase Supplementation on Performance of Broilers Fed Different Levels of Phosphorus. Korean J. Poult. Sci. Vol. 27, No.3 169-179.
  5. Lim, H. S., H. Namkung and I. K. Paik. 2002. Effects of phytase supplementation on the performance, egg quality and phosphorus excretion of laying hens fed different levels of dietary calcium and non-phytate phosphorus. In press. Poult. Sci.
  6. Lim, H. S., H. Namkung, J. S. Um, K. R. Kang, B. S. Kim and I.K. Paik. 2001. The Effects of Phytase Supplementation on the Performance of Broiler Chickens Fed Diet with Different Levels of Non-Phytate Phosphorus. Asian-Aust. J. Anim. Sci. 14(2):250-257.
  7. Schoener, B. R. J., P. P. Hoppe, G. Schwarz, and H. Wieshe. 1993. Effects of microbial phytase and inorganic phosphate in broiler chickens: performance and mineral retention at various calcium levels. J. Anim. Physiol. Anim. Nutr. 69:235-244.
  8. Simons, P. C. M., A. W. Jongbloed, H. A. J. Versteegh and P. A. Kemme. 1992. Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br. J. Nutr. 66:100-109
  9. Taylor, T. G., and C. G. Dacke. 1984. Calcium metabolism and its regulation. Pages 126-170 in: Physiology and Biochemistry of the domestic Fowl. B. M. Freeman, ed. Academic Press, London, UK.
  10. Um, J. S. and I. K. Paik. 1999. Effects of Microbial Phytase Mineral Retention of Laying Hens Fed Different Levels of Phosphorus. Poult. Sci. 78:75-79.
  11. Pointillart, A. 1993. Importance of phytates and cereal phytases in the feeding of pig. Enzymes in Animal Nutrition. Bio/Technology. 192-198.
  12. Simons, P. C. M. and H. A. J. Versteegh. 1993. Role of phytase in poultry nutrition. Enzymes in Animal Nutrition. Proceedings of the 1st Symposium (Ed C. Wenk and M. Boessinger). Kartause Ittingen. Switzerland. pp.181-186.
  13. Van der Klis, J. D. and H. A. J. Versteegh. 1996. Phosphorus nutrition of poultry. Pages 71-83. in: Recent advanced in animal nutrition. P. C. Gransworthy and D. J. A. Cole, ed. Nottingham, UK.
  14. Williams, P. E. V. and J. M. Kelly. 1994. Animal production and pollution problems. Livestock Production for the 21st Century: Priorities and Research Needs. (Ed. P. A. Thacker). pp. 159-186.
  15. Paik, I. K., J. S. Um, S. J. Lee and J. G. Lee. 2000. Evaluation of the Efficacy of Crude Phytase Preparations in Broiler Chickens. Asian-Aus. J. Anim. Sci. 13(5):673-680.
  16. Pen, J., T. C. Verwoerd, P. A. Paeiden, R. F. Beudeker, P. J. M. van den Elzen, K. Geerse, J. D. van der Klis, H. A. J. Versteegh, J. J. van Ooyen and A. Hoekema. 1993. Phytasecontaining transgenic seeds as a novel feed additive for improved phosphorus utilization. Bio/Technology. 11:811-814
  17. Schaefer, J. 1977. Sampling, characterization and analysis of malodours. Agric. Environ. 3: 121-127.
  18. Sebastian, S., S. P. Touchburn, E. R. Chavez, and P. C. Lague. 1996a. Efficacy of supplemental microbial phytase at different dietary calcium levels on growth performance and mineral utilization of broiler chickens. Poultry Science 75:1516-1523.
  19. Gordon, R. W. and D. A. Roland, SR. 1997. Performance of commercial laying hens fed various phosphorus levels with and without supplement phytase. Poult. Sci. 76:1172-1177.
  20. Hill, R. and C. Tyler. 1954b. The influence of time, temperature, pH and calcium carbonate on the activity of the phytase of certain cereals. J. Agric. Sci. 44:306-310.
  21. Kim, B. H and I. K. Paik. 2002. Influence of hydrothermal treatment of wheat bran on phytate-P content and performance of broiler chickens. In press. Kor. J. Anim. Sci.
  22. Reddy, N. R., C. V. Balakrishnan and D. K. Salunkhe. 1982. Phytates in legumes. Adv. Food Res. 28:1-92.
  23. Ryu, S. and T. G. Park. 1998. Thermal stabilization of Aspergillus phytase by L-arginine. Biotechnol. Bioprocess Eng. 3:32-34.
  24. Edwards, H. M. J. 1993. Dietary 1,25-dihydroxycholecalciferol supplementation increase natural phytate phosphorus utilization in chickens. J. Nutr. 103:567-577.
  25. Engelen, A. J., F. C. Van der Heeft, P. H. G. Randsdorp and E. L. C. Smit. 1994. Single and rapid determination of phytase activity. J. AOAC. Int 77(3):760-764.
  26. Jongbloed, A. W. 1989. Phytase can increase P-digestibility. Pigs-Misset 21.
  27. Keteran, P. P., E. S. Batterham, E. B. Dettmann and D. J. Farrell. 1993. Phosphorus studies in pigs : Effect of phytase supplementation on the digestibility and availability of phosphorus in soya bean meal for grower pigs. Br. J. Nutr. 70:289-311.
  28. Peter, W. and H. Jeroch. 1993. The effectiveness of microbial phytase addition to layer rations on maize and wheat basis. Enzymes in Animal Nutrition. Bio/Technology. pp. 206-209.
  29. Um, J. S., I. K. Paik M. B. Chang and B. H. Lee. 1999. Effects of microbial phytase supplementation to diets with low nonphytate phosphorus levels on the performance and bioavailability of nutrients in laying hens. Asian-Aust. J. Anim. Sci. 12(2):203-208.
  30. Biehl, R. R., D. H. Baker and H. F. DeLuca. 1995. $1{\alpha}$-Hydroxylated cholecalciferol compounds act additively with microbial phytase to improve phosphorus, zinc and manganese utilization in chicks fed soy-based diets. J. Nutr. 125:2407-2416.
  31. Jongbloed, A. W. and N. P. Lenis. 1992. Alteration of nutrition as a means to reduce environmental pollution by pigs. Livestock Prod. Sci. 31:75-94.
  32. Um, J. S., H. S. Lim, S. H. Ahn and I. K Paik. 2000. Effects of Microbial Phytase Supplementation to Low Phosphorus Diets on the Performance and Utilization of Nutrients in Broiler Chickens. Asian-Aus. J. Anim. Sci. 13(6):824-829
  33. Hill, R. and C. Tyler. 1954a. The effect of decreasing acidity on the solubility of calcium, magnesium and phosphorus in bran and certain pure salts solution. J. Agric. Sci. 44:311-323.
  34. Mitchell, R. D. and H. M. Edwards, Jr. 1996b. Effects of phytase and 1,25-Dihydroxycholecalciferol on phytate utilization and the quantitative requirement for calcium and phosphorus in young broiler chickens. Poult. Sci. 75:95-110.
  35. Nasi, M. 1991. Plant phosphorus responses to supplemental microbial phytase in the diet of the growing pig. Pages 114-119 in: Digestive physiology in pig. Proc. 5th Symp. Wageningen.
  36. Oberleas, D. and B. F. Harland. 1981. Phytate content of foods: Effect on dietary zinc bioavailability. J. Anim. Diet. Assoc. 79:44-447.
  37. Simons, P. C. M., H. A. J. Versteegh, A. W. Jongbloed, P. A. Kemme, P. Slump, K. D. Bos, M. G. E. Wolters, R. F. Buedeker and G. J. Verchoor. 1990. Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br. J. Nutr. 64:525-540.
  38. Mohammed, A., M. J. Gibney and T. G. Taylor. 1991. The effects of dietary levels of inorganic phosphorus, calcium and cholecalciferol on the digestibility of phytate-P by the chick. Br. J. Nutr. 66:251-259.
  39. Mroz, Z., A. W. Jongbloed and P. A. Kemme. 1994. Apparent digestibility and retention in nutrients bound to phytate complexes as influences by microbial phytase and feeding regimen in pig. J. Anim. Sci. 72:126-132.
  40. Zyla, K. and J. Koreleski. 1993. In-vitro and in-vivo dephosphorylation of rapeseed meal by means of phytatedepleting enzymes derived from Aspergillus niger. J. Sci. Food Agric. 61: 1-6.
  41. Sebastian, S., S. P. Touchburn, E. R. Chavez and P. C. Lague. 1996b. The effects of supplemental microbial phytase on the performance and utilization of dietary calcium, phosphorus, copper, and zinc in broiler chickens fed corn-soybean diets. Poultry Science 75:729-736.
  42. Van der Klis, J. D., H. A. J. Versteegh and C. W. Scheele. 1994. ractical enzyme use in poultry diets: phytase and NSP enzymes. Pages 113-128 in: BASF Technical symposium during the Carolina Poultry Nutrition Conference, Charlotte, NC.
  43. Adeola, O. 1995. Digestive utilization of minerals by weaning pigs fed copper and phytase supplemented diets. Can. J. Anim. Sci. 75:603-610.
  44. Broz, J., P. Oldale, A. H. Perrin-Voltz, G. Rychen, J. Schulze and C. Simoes Nunes. 1994. Effects of supplementation phytase on performance and phosphorus utilisation in broiler chickens fed a low phosphorus diet without addition of inorganic phosphates. Br. Poult. Sci. 35:273.
  45. Qian, H. E. T. Kornegay and D. M. Dendow. 1996. Phosphorus equivalence of microbial phytase in turkey diets as influenced by calcium to phosphorus ratios and phosphorus levels. Poultry Science 75:69-81.
  46. Lei, X. G., P. K. Ku, E. R. Miller, M. T. Yokoyama and D. E. Ullrey. 1994. Calcium level affects the efficacy of supplemental microbial phytase in corn-soybean meal diets of weaning pigs. J. Anim. Sci. 72:139-143.
  47. Yi. Z, Kornegay. E. T, Ravindran. V, and Cenbow. D. M. 1996. Improving phytate phosphorus availability in corn and soybean meal for broilers using microbial phytase and calculation of phosphorus equivalency values for phytase. Poultry Science 75:240-249.
  48. Pallauf, J., D. Hohler, G. Rimbach. 1992. Effect of microbial phytase supplementation to a maize-soya-diet on the apparent absorption of Mg, Fe, Cu, Mn and Zn and parameters of Znstatus in piglets. J. Anim. Physiol. Anim. Nutr. 68:1-9.
  49. Young, L. G., M. Leunissen and J. L. Atkinson. 1993. Addition of microbial phytase to diets of young pigs. J. Anim. Sci. 71:2147-2150.
  50. Annison, G. and M. Choct. 1993. Enzymes in poultry diets. Enzymes in Animal Nutrition. (Ed. C. Wenk and M. Boessinger). Proceedings of the 1st Symposium. Kartause Ittingen, Switzerland. pp. 61-68.
  51. Mitchell, R. D. and H. M. Edwards, Jr. 1996a. Additive effects of 1,25-Dihydroxycholecalciferol and phytase on phytate phosphorus utilization and related parameters in broiler chickens. Poult. Sci. 75:111-119.

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