- Volume 16 Issue 10
DOI QR Code
Study on Genetic Variation of 4 Microsatellite DNA Markers and Their Relationship with Somatic Cell Counts in Cow Milk
- Jin, Hai-Guo (College of Agriculture, Yanbian University) ;
- Zhou, Guo-li (Department of Biology, Liaocheng University) ;
- Yang, Cao (College of Agriculture, Yanbian University) ;
- Chu, Ming-Xing (Research Institute of Animal Science, Chinese Academy of Agricultural Sciences)
- Received : 2002.08.20
- Accepted : 2003.05.28
- Published : 2003.10.01
Four microsatellite DNA loci BM1818, BM1258, BM1443 and BM1905 associated with the somatic cell counts (SCC) in cow milk were analyzed for genetic variation in 240 Beijing Holstein cows. The PCR amplified products of microsatellites DNA were detected by non-denatured polyacrylamide gel electrophoresis. The number of alleles for BM1818, BM1258, BM1443 and BM1905 were 4, 5, 8 and 6 in Beijing Holstein cows, respectively. The allele size ranges for BM1818, BM1258, BM1443 and BM1905 were 274 bp to 286 bp, 92 bp to 106 bp, 154 bp to 170 bp and 187 bp to 201 bp, respectively. The polymorphism information content/effective number of alleles/heterozygosity for BM1818, BM1258, BM1443 and BM1905 were 0.3869/1.7693/0.4348, 0.5923/2.9121/0.6566, 0.7114/3.9012/0.7437 and 0.5921/2.8244/0.6459. These data showed the microsatellite DNA locus BM1443 has the highest variability, followed by BM1258, BM1905 and BM1818. The results of the least squares means analysis showed as follows: the least squares mean of SCC for BM1818 284 bp/284 bp was significantly lower than that for BM1818 286 bp/286 bp (p<0.05). The least squares mean of SCC for BM1258 100 bp/100 bp was significantly lower than that for BM1258 102 bp/102 bp, 106 bp/106 bp, 106 bp/104 bp, 106 bp/102 bp, 106 bp/100 bp, 104 bp/100 bp (p<0.05). The least squares mean of SCC for BM1443 166 bp/160 bp and 166 bp/166 bp was significantly lower than that for BM1443 170 bp/160 bp, 160 bp/157 bp, 165 bp/160 bp (p<0.05). The least squares mean of SCC for BM1905 187 bp/187 bp was significantly lower than that for BM1905 197 bp/195 bp, 193 bp/187 bp (p<0.05).
- Ashwell, M. S. and C. P. Van Tassell. 1999. Detection of putative loci affecting milk, health, and type traits in a US Holstein population using 70 microsatellite markers in a genome scan. J. Dairy Sci. 82:2497-2502.
- Emanuelson, U., B. Danell and J. Philipsson. 1988. Genetic parameters for clinical mastitis, somatic cell counts and milk production estimated by multiple-trait restricted maximum likelihood. J. Dairy Sci. 71:467-476.
- Shook, G. E. 1986. Approaches to summarizing somatic cell counts which improve interpretability. Proc. Natl. Mastitis Countil, Arlington, VA. pp. 150-166.
- Strauss, W. M. 1991. Preparation of genomic DNA from mammalian tissue. In: Current Protocols in molecular Biology (Ed. F. M. Ausubel, R. Brent, R. E. Kingston et al.) Vol. 1, pp. 2.2.1-2.2.3. John Wiley and Sons. New York, NY., USA.
- Philipsson, J., G. Ral and B. Berglund. 1995. Somatic cell count as a selection criterion for mastitis resistance in dairy cattle. Livest. Prod. Sci. 41:195-200.
- Shook, G. E. 1986. Genetic aspecs of mastitis. Proc Natl Mastitis Countil, Arlington , VA, pp. 68-77.
- Bishop, M. D., S. M. Kappes, J. W. Keele, R. T. Stone, S. L. F. Sunden, G. A. Hawkins, S. S. Toldo, R. Fries, M. D. Grosz, J. Yoo and C. W. Beattie. 1994. A genetic linkage map for cattle. Genetics. 136:619-639.
- Lund, T., F. Miglior and J. C. M. Dekkers. 1994. Genetic relationships between clinical mastitis, somatic cell count and udder conformation in Danish Holsteins. Livest. Prod. Sci. 39:243-251.
- Reents, R., J. Jamrozik and L. R. Schaeffer. 1995. Estimation of genetic parameters for test day records of somatic cell score. J. Dairy Sci. 78:2847-2857.
- Schutz, M. M., L. B. Hansen and G. R. Steuernagel. 1990. Genetic parameters for somatic cells, protein and fat in milk of Holsteins. J. Dairy Sci. 73:494-502.
- Arranz, J. J., Y. Bayon and F. San Primitivo. 1996. Genetic variation at five microsatellite loci in four breeds of cattle. Journal of Agricultural Science (Cambridge). 127:533-538.
- Shook, G. E. and M. M. Schutz. 1994. Selection on somatic cell score to improve resistance to mastitis in the United States. J. Dairy Sci. 77:648-658. https://doi.org/10.3168/jds.S0022-0302(94)76995-2
- Ashwell, M. S., C. P. Van Tassell and T. S. Sonstegard. 2001. A genome scan to identify quantitative trait loci affecting economically important traits in a US Holstein population. J. Dairy Sci. 84(11):2535-2542.
- Crawford, A. M., F. C. Buchanan and P. A. Swarbrick. 1991. The use of dinucleotide repeats or microsatellites as genetic markers in domestic animals. Proceedings of the New Zealand Society of Animal Production. 51:79-83.
- Hines, H. C., J. P. Zikakis and G. F. Haenlein. 1981. Linkage relationships among loci of polymorphisms in blood and milk of cattle. J. Dairy Sci. 64(1):71-76.
- Martin-Burriel, I., E. Garcia-Muro and P. Zaragoza. 1999. Genetic diversity analysis of six Spanish native cattle breeds using microsatellites. Animal Genetics. 30:177-182.
- Van Zeveren, A., L. Peelman, A. van de Weghe and Y. Bouquet. 1995. A genetic study of four Belgian pig populations by means of seven microsatellite loci. J. Anim. Breed. Genet. 112:191-204.
- Botstein, D., R. L. White, M. Skolnick and R. W. Davis. 1980. Construction of a genetic linkage map in human using restriction fragment length polymorphisms. Anim. J. Hum. Genet. 32:314-331.
- Nei, M. and A. K. Roychoudhury. 1974. Sampling variances of heterozygosity and genetic distance. Genetics. 76:379-390.
- Zhang, W. C., J. C. M. Dekkers and G. Banos. 1994. Adjustment factors and genetic evaluation for somatic cell score and relationships with other traits of Canadian Holsteins. J. Dairy Sci. 77:659-665.
- Davis, G. P., D. J. S. Hetzel and N. J. Corbet. 1998. The mapping of quantitative trait loci for birth weight in a tropical beef herd. In: Proceedings of the 6th World Congress on Genetics Applied to Livestock Production, Armidale and NSW, AUSTRALIA. 26:441-444.
- Weller, J. I., A. Saran and Y. Zeliger. 1992. Genetic and environmental relationships among somatic cell count, bacterial infection, and clinical mastitis. J. Dairy Sci. 75:2532-2540.
- Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
- SAS Institute Inc. 1990. SAS/STAT Uer’s Guide: Version 6. 4th Edition, Volume 1. SAS Institute Inc., Cary, North Carolina, USA. pp. 891-996.
- Shook, G. E. 1993. Genetic improvement of mastitis through selection on somatic cell count, Veterinary Clinics of North America: Food Animal Practice, Vol. 9. 563-581.
- Shook, G. E. 1989. Selection for disease resistance. J. Dairy Sci. 72:1349-1362. https://doi.org/10.3168/jds.S0022-0302(89)79242-0
- Barker, J. S. F. 1994. A global protocol for determining genetic distances among domestic livestock breeds. In: Proceedings of the 5th World Congress on Genetics Applied to Livestock Production, Guelph, and Ontario, Canada. 21:501-508.
- Fries, R., A. Eggen and J. E. Womack. 1993. The bovine genome map. Mamm. Genome. 4:405-428.
- Lu, S. D. 1993. An experiment technology of molecular biology. Higher Education Publishing Company, Beijing, China.
- Schutz, M. M. 1994. Genetic evaluation of somatic cell scores for United States dairy cattle. J. Dairy Sci. 77:2113-2129. https://doi.org/10.3168/jds.S0022-0302(94)77154-X
- Coffey, E. M., W. E. Vinson and R. E. Pearson. 1986. Potential of somatic cell concentration in milk as a sire selection criterion to reduce mastitis in dairy cattle. J. Dairy Sci. 69:2163-2172.
- Status of bovine mastitis and associated risk factors in subtropical Jeju Island, South Korea vol.45, pp.8, 2013, https://doi.org/10.1007/s11250-013-0422-3
- Molecular markers for resistance against infectious diseases of economic importance vol.10, pp.1, 2017, https://doi.org/10.14202/vetworld.2017.112-120