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The Role of Phosphatidylinositol 3-kinase and Mitogenic Activated Protein Kinase on the Differentiation of Ovine Preadipocytes

  • Choi, K.C. (Dept. of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Shrestha, S.G. (Dept. of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Roh, S.G. (Dept. of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Hishikawa, D. (Dept. of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Kuno, M. (Dept. of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Tsuzuki, H. (Dept. of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Hong, Y.H. (Dept. of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Sasaki, S. (Dept. of Food Production Science, Faculty of Agriculture, Shinshu University)
  • Received : 2002.08.02
  • Accepted : 2003.03.18
  • Published : 2003.08.01

Abstract

The aim of this study was to investigate the role of phosphatidylinositol 3-kinase (PI3 kinase) and the mitogenactivating protein (MAP) kinase pathway on the differentiation of ovine preadipocytes. In order to investigate this issue, we monitored glycerol 3-phosphate dehydrogenase (GPDH) activity during differentiation with specific inhibitors of PI3 kinase and MAP kinase-Erk kinase, LY294002 and PD098059, respectively. The preadipocytes, which were obtained from ovine subcutaneous adipose tissues, were proliferated to confluence and then differentiated to adipocytes in differentiation medium with each inhibitor for 10 days. The confluent preadipocytes and differentiated adipocytes at days 3, 7 and 10 were harvested for assay of GPDH activity. LY294002 inhibited the differentiation program in dose- and day-dependent manners during 10 days of differentiation. PD098059 did not affect GPDH activity during differentiation. Furthermore, the expression of peroxisome proliferator-activated receptor ${\gamma}2$ (PPAR-${\gamma}2$), the representative early gene of differentiation, was markedly reduced by LY294002 treatment, although PD098059 did not change it. Our results demonstrated that the activation of PI3 kinase contributes to the differentiation process of ovine preadipocytes.

Keywords

Sheep;Preadipocytes;Differentiation;PI3 Kinase;MAP Kinase

Acknowledgement

Supported by : Sasakawa Scientific Research

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