DOI QR코드

DOI QR Code

Relationship between Peripheral Plasma Inhibin and Progesterone Concentrations in Sahiwal Cattle (Bos Indicus) and Murrah Buffaloes (Bubalus bubalis)

  • Mondal, S. (National Institute of Animal Nutrition and Physiology) ;
  • Prakash, B.S. (National Dairy Research Institute) ;
  • Palta, P. (National Dairy Research Institute)
  • Received : 2002.06.03
  • Accepted : 2002.09.05
  • Published : 2003.01.01

Abstract

The present study investigated peripheral plasma immunoreactive inhibin (ir-inhibin) concentrations in relation to the stage of oestrous cycle and progesterone concentrations in cycling Sahiwal cattle (Bos indicus) and Murrah buffaloes (Bubalus bubalis). Blood samples were collected once daily for thirty-two consecutive days from cattle and buffaloes (5 each) during winter months of January and February. Mean (${\pm}$S.E.M.) plasma ir-inhibin concentrations ranged from $0.40{\pm}0.01$ to $0.59{\pm}0.03ng/ml$ in cattle and from $0.29{\pm}0.03$ to $0.52{\pm}0.05ng/ml$ in buffaloes. In cattle, ir-inhibin concentrations increased from $0.47{\pm}0.07ng/ml$ on day -4 (day 0=day of oestrus) to reach a maximum value of $0.59{\pm}0.03ng/ml$ on day -2. Thereafter, ir-inhibin concentrations showed a decline to reach a low of $0.40{\pm}0.01ng/ml$ on day 11 of the oestrous cycle. In buffaloes, ir-inhibin concentrations increased from $0.38{\pm}0.04 ng/ml$ on day -4 to reach a maximum concentration of $0.52{\pm}0.05ng/ml$ on day -2. Ir-inhibin concentrations then declined to reach a low of $0.29{\pm}0.03ng/ml on day 9 of the cycle. In both cattle and buffaloes, ir-inhibin concentrations which were lowest ($0.43{\pm}0.02$ and $0.34{\pm}0.02ng/ml$, respectively) during the mid-luteal phase of the oestrous cycle increased (p<0.05) to $0.52{\pm}0.03$ and $0.44{\pm}0.04ng/ml$, respectively, during the late luteal phase, and then further to the highest value of $0.53{\pm}0.02$ and $0.49{\pm}0.04ng/ml$, respectively, during the perioestrus phase, following which these declined to $0.50{\pm}0.02$ and $0.39{\pm}0.03ng/ml$, respectively, during the early luteal phase. The variations in peripheral plasma ir-inhibin profile in both the species appear to be related to the changes in characteristics of follicular populations during the oestrous cycle. Peripheral plasma ir-inhibin concentrations were negatively correlated with progesterone concentrations in cattle (r=-0.51, p<0.01) and buffaloes (r=-0.30, p<0.01) indicating that the corpus luteum is not a source of peripheral ir-inhibin in these species.

Keywords

Buffalo;Cattle;Inhibin;Progesterone

References

  1. Findlay, J. K., I. J. Clark and D. M. Robertson 1990. Inhibin concentrations in ovarian and jugular plasma and the relationship of inhibin with FSH and LH during ovine estrous cycle. Endocrinology 126:528-535. https://doi.org/10.1210/endo-126-1-528
  2. Ginther, O. J., J. P. Kastelic and L. Knopf. 1989. Composition and characteristics of follicular waves during the bovine estrous cycle. Anim. Reprod. Sci. 20:187-200. https://doi.org/10.1016/0378-4320(89)90084-5
  3. Good, T. E. M., P. S. D. Weber, J. L. H. Ireland, J. Pulaski, V. Padmanabhan, A. L. Schneyer, G. Lambert-Messerlian, B. R. Ghosh, W. I. Miller, N. Groome and J. J. Ireland. 1995. Isolation of nine different biologically and immunologically active molecular variants of bovine follicular inhibin. Biol. Reprod. 53:1478-1488. https://doi.org/10.1095/biolreprod53.6.1478
  4. Kaneko, H., G. Watanabe, K. Taya and S. Sasamoto. 1992. Changes in peripheral levels of bioactive and immunoreactive inhibin, estradiol-$17{\beta}$, progesterone, luteinizing hormone and follicle stimulating hormone associated with follicular development in cows induced to superovulate with equine chorionic gonadotropin. Biol. Reprod. 47:76-82. https://doi.org/10.1095/biolreprod47.1.76
  5. Mondal, S., B. S. Prakash and P. Palta. 2000b. Relationship between peripheral inhibin and FSH in cycling Murrah buffaloes (Bubalus bubalis). J. Endocrinol. 167:67.
  6. Palta, P., N. Bansal, B. S. Prakash, R. S. Manik and M. L. Madan. 1998. Follicular fluid inhibin in relation to follicular diameter and estradiol-17$\beta$, progesterone and testosterone concentrations in individual buffalo ovarian follicles. Ind. J. Exptl. Biol. 36:768-774.
  7. Robertson, D. M., S. Hayward, D. Irby, J. Jacobson, L. Clarke and R. I. McLachlan and D. M. DeKretser. 1988. Radioimmunoassay of rat serum inhibin: changes after PMSG stimulation and gonadectomy. Mol. Cell. Endocrinol. 58:1-8. https://doi.org/10.1016/0303-7207(88)90047-0
  8. Taya, K., H. Kaneko, G. Watanabe and S. Sasamoto. 1991. Inhibin and secretion of FSH in estrous cycles of cows and pig. J. Reprod. Fertil. (Suppl.) 43:151-162.
  9. Torney, A. H., Y. Hodgson, R. Forage and D. M. DeKretser. 1989. Cellular localization of inhibin mRNA in the bovine ovary by in situ hybridization. J. Reprod. Fertil. 86:391-399. https://doi.org/10.1530/jrf.0.0860391
  10. Ireland, J. L. H., T. E. M. Good, P. G. Knight and J. J. Ireland. 1994. Alterations in amounts of different forms of inhibin during follicular atresia. Biol. Reprod. 50:1265-1276. https://doi.org/10.1095/biolreprod50.6.1265
  11. Claus, R., H. Karg, D. Zwiauer, I. V. Butler, F. Pirchner and E. Rattenberger. 1983. Analysis of factors influencing reproductive performance of the dairy cows by progesterone assay in milk fat. Br. Vet. J. 139:29-37.
  12. Guilbault, L. A., P. Rouillier, P. Matton, R. G. Glencross, A. J. Beard and P. G. Knight. 1993. Relationship between the level of atresia and inhibin contents ($\alpha$- subunit and $\alpha$-$\beta$ dimer) in morphologically dominant follicles during their growing and regressing phases of development in cattle. Biol. Reprod. 48: 268-276. https://doi.org/10.1095/biolreprod48.2.268
  13. O'shea, T., A. Hillard, S. T. Anderson, B. M. Bindon, J. K. Findlay, C. G. Tsonis and J. K. Wilkins. 1994. Inhibin immunization for increasing ovulation rate and superovulation. Theriogenology 41:3-17. https://doi.org/10.1016/S0093-691X(05)80043-3
  14. Palta, P., B. S. Prakash and M. L. Madan. 1996a. Peripheral inhibin levels during estrous cycle in Murrah buffalo (Bubalus bubalis). Theriogenology 45:655-664. https://doi.org/10.1016/0093-691X(95)00411-Z
  15. Pierson, R. A. and O. J. Ginther. 1987. Follicular populations during the oestrous cycle in heifers. I. Influence of day. Anim. Reprod. Sci. 14:165-176. https://doi.org/10.1016/0378-4320(87)90080-7
  16. Burger, H. G. 1992. Inhibin. Reprod. Med. Rev. 1:1–20.
  17. Palta, P., M. Kumar, S. Jailkhani, R. S. Manik and M. L. Madan. 1997b. Changes in peripheral inhibin levels and follicular development following treatment of buffalo with PMSG and Neutra-PMSG for superovulation. Theriogenology 48:233-240. https://doi.org/10.1016/S0093-691X(97)84070-8
  18. Robertson, D. M., M. Giacometti, L. M. Foulds, J. Lahnstein, N. H. Goss, M. T. W. Hearn and D. M. DeKretser. 1989. Isolation of inhibin $\alpha$-subunit precursor proteins from bovine follicular fluid. Endocrinology 125:2141-2149. https://doi.org/10.1210/endo-125-4-2141
  19. Sunderland, S. J., P. G. Knight, M. P. Boland, J. F. Roche and J. J. Ireland. 1996. Alterations in intrafollicular levels of different molecular mass forms of inhibin during development of follicular- and luteal-phase dominant follicles in heifers. Biol. Reprod. 54:453-462. https://doi.org/10.1095/biolreprod54.2.453
  20. Rodgers, R. J., S. J. Stuchbery and J. K. Findlay. 1989. Inhibin mRNA in ovine and bovine ovarian follicles and corpora lutea throughout estrous cycle and gestation. Mol. Cell. Endocrinol. 62:95-101. https://doi.org/10.1016/0303-7207(89)90117-2
  21. Hoffman, B., O. Gunzler, R. Hamburger and W. Schmidt. 1976. Milk progesterone as a parameter for fertility control in cattle: methodological approaches and present status of application in Germany. Br. Vet. J. 132:469-476. https://doi.org/10.1016/S0007-1935(17)34584-0
  22. Palta, P., B. S. Prakash, R. S. Manik and M. L. Madan. 1996b. Inhibin in individual buffalo ovarian follicles in relation to size. Ind. J. Exptl. Biol. 34:606-608.
  23. Palta, P., S. Mondal, B. S. Prakash and M. L. Madan. 1997a. Peripheral inhibin levels in relation to climatic variations and stage of estrous cycle in buffalo (Bubalus bubalis). Theriogenology 47:989-995. https://doi.org/10.1016/S0093-691X(97)00055-1
  24. Campbell, B. K., A. S. McNeilly, G. E. Mann and D. T. Baird. 1991. The effect of stage of estrous cycle and follicular maturation on ovarian inhibin production in sheep. Biol. Reprod. 44:483-490. https://doi.org/10.1095/biolreprod44.3.483
  25. Manik, R. S., S. K. Singla, P. Palta and M. L. Madan. 1998a. Real time ultrasound evaluation of changes in follicular populations during oestrous cycle in buffalo. Ind. J. Anim. Sci. 68:1157-1159.
  26. Foote, R. H., E. A. B. Oltenacu, H. L. Kimmerfeld, R. D. Smith, P. M. Riek and R. K. Braun. 1979. Milk progesterone as diagnostic aid. Br. Vet. J. 135:550-558. https://doi.org/10.1016/S0007-1935(17)30009-X
  27. Kamboj, M. and B. S. Prakash. 1993. Relationship of progesterone in plasma and whole milk of buffaloes during cyclicity and early pregnancy. Trop. Anim. Hlth. Prod. 25:185-192. https://doi.org/10.1007/BF02236239
  28. Manik, R. S., S. K. Singla, P. Palta and M. L. Madan. 1998b. Ovarian follicular dynamics monitored by real-time ultrasonography during oestrous cycle in buffalo (Bubalus bubalis). Asian-Aus. J. Anim. Sci. 11:480-485. https://doi.org/10.5713/ajas.1998.480
  29. Martin, T. L., R. L. Fogwell and J. J. Ireland. 1991. Concentrations of inhibins and steroids in follicular fluid during development of dominant follicles in heifers. Biol. Reprod. 44:693-700. https://doi.org/10.1095/biolreprod44.4.693
  30. Knight, P. G., S. Muttukrishna and N. P. Groome. 1996. Development and application of a two-site enzyme immunoassay for the determination of 'total' activin-A concentrations in serum and follicular fluid. J. Endocrinol. 148:267-279. https://doi.org/10.1677/joe.0.1480267
  31. Snedecor, G. W. and Cochran, W. G. 1967. Statistical Methods 6th Edn. Oxford and IBH Publication Co., New Delhi, India.
  32. Mondal, S., P. Palta and B. S. Prakash. 2000a. Peripheral inhibin concentrations in relation to stage of estrous cycle and progesterone in Sahiwal cows and Murrah buffaloes. In Proceedings of 14th International Congress on Animal Reproduction, Stockholm, Sweden Volume 1, p. 209.
  33. Palta, P. 1998. Fertility augmentation by inhibin-based fecundity vaccines: potential applications in buffaloes. Buff. J. 14:1-19.
  34. Campbell, B. K., G. E. Mann, A. S. McNeilly and D. T. Baird. 1990. The pattern of ovarian inhibin, estradiol and androstenedione secretion during estrous cycle of the ewe. Endocrinology 127:227- 235. https://doi.org/10.1210/endo-127-1-227

Cited by

  1. Seasonal variation and circadian rhythmicity of the prolactin profile during the summer months in repeat-breeding Murrah buffalo heifers vol.19, pp.4, 2007, https://doi.org/10.1071/RD06093