Bile and Acid Tolerance of Lactic Acid Bacteria Isolated from Dadih and Their Antimutagenicity against Mutagenic Heated Tauco

  • Pato, Usman (Faculty of Agriculture, University of Riau)
  • Received : 2002.11.14
  • Accepted : 2003.07.16
  • Published : 2003.11.01


Antimutagenicity of milk cultured with lactic acid bacteria isolated from dadih on the mutagenicity of heated salty and sweet tauco was examined using streptomycin dependent (SD) 510 strain of Salmonella typhimurium TA 98 as a tester culture. Cultured milk samples exhibited widely antimutagenic activity against mutagenic heated salty and sweet tauco. Lc. lactis subsp. lactis R-22, Lc. lactis subsp. casei R-35, Lc. lactis subsp. casei R-52 and E. faecalis subsp. liquefaciens R-55 exhibited no inhibitory effect on the mutagenic heated salty tauco. Mutagenicity of heated sweet tauco was inhibited by cultured milks stronger than that of heated salty tauco. Milk cultured with Lc. lactis subsp. cremoris R-48, Leuc. mesentroides R-51 and Lc. lactis subsp. casei R-68 showed high inhibition against the mutagenicity of both heated salty and sweet taucos. Antimutagenic activity of the cultured milks against mutagenic heated tauco was attributed to the bacterial cells. Among the three strains which showed high antimutagenicity, only Leuc. mesentroides R-51 was tolerant to both acid and bile; so this strain can be used as probiotic in preventing the occurrence of mutagenesis caused by mutagenic heated food like tauco.


  1. Fernandes, C. F., K. M. Shahani and M. A. Amer. 1987. Therapeutic role of dietary lactobacilli and lactobacillic fermented dairy products. FEMS Microbiol. Rev. 46:343-356.
  2. Itoh, T. 1992. Functional benefits from lactic acid bacteria used in cultured milk. Anim. Sci. Technol. (Jpn.) 63:1276-1289.
  3. Maron, D. M. and B. N. Ames. 1983. Revised methods for the Salmonella mutagenicity test. Mutat. Res. 113:173-215.
  4. Surono, I. S. and A. Hosono. 1996. Antimutagenicity of milk cultured with lactic acid bacteria from dadih against mutagenic terasi. Milchwissenschaft 51:493-497.
  5. Thyagaraja, N. and A. Hosono. 1993. Antimutagenicity of lactic acid bacteria from idly against food-related mutagens. J. Food Prot. 56:1061-1066.
  6. Hosono, A., A. Yoshimura and H. Otani. 1987. Antimutagenic activity of cellular component of Streptococcus faecalis IFO 12965. Neth. Milk Dairy J. 41:239-245.
  7. Usman and A. Hosono. 1999. Bile tolerance, taurocholate deconjugation and binding of cholesterol by Lactobacillus gasseri strains. J. Dairy Sci. 82:243-248.
  8. Usman and A. Hosono. 1996. Chemical and bacteriological composition of tauco, a traditional fermented food in Indonesia. Quest Tradit. Food 16:41-50.
  9. Gohran, M. 1994. Lactobacillus with the ability of colonization and establishing in the intestinal tracts. Japan Patent 6-501624.
  10. Hood, S. K. and E. A. Zottola. 1988. Effect of low pH on the ability of Lactobacillus acidophilus to survive and adhere to human intestinal cells. J. Food Sci. 53:1514-1516.
  11. Hosoda, M., H. Hashimoto, H. Morita, M. Chiba and A. Hosono. 1992. Antimutagenicity of milk cultured with lactic acid bacteria against N-methyl, N'-nitro, N-nitrosoguanidine. J. Dairy Sci. 75:976-981.
  12. Usman and A. Hosono. 1997. Antimutagenic activity of Maillard reaction products against mutagenic heated tauco. Ital. J. Food Sci. 4:268-276.
  13. Hosono, A., S. Sagae and F. Tokita. 1986c. Desmutagenic effect of cultured milk on chemically induced mutagenesis in Escherichia coli B/r WP2 trp- hcr-. Milchwissenchaft 41:142-145.
  14. Renner, H. W. and R. Munzner. 1991. The possible role of probiotics as dietary antimutagens. Mutat. Res. 262:239-245.
  15. Surono, I. S. 2003. In vitro probiotic properties of indigenous dadih lactic acid bacteria. Asian-Aust. J. Anim. Sci. 16:726-731.
  16. Walker, D. R. and S. E. Gilliland. 1993. Relationship among bile tolerance, bile salt deconjugation, and assimilation of cholesterol by Lactobacullus acidophilus. J. Dairy Sci. 76:956-961.
  17. Bodana, A. R. and D. R. Rao. 1990. Antimutagenic activity of milk fermented by Streptococcus thermophilus and Lactobacillus bulgaricus. J. Dairy Sci. 73:3379-3384.
  18. Hosono, A., E. Omote, Y. Izawa, F. Tokita and T. Kada. 1986b. Isolation of spontaneous induced-streptomycin dependent mutants of Salmonella typhimurium TA 98 and TA 100 strains and desmutagenicity tests in vitro of cultured milk. Lebensm.-Wiss. u-Technol. 19:161-163.
  19. Sreekumar, O. and A. Hosono. 1998. Antimutagenicity and the influence of physical factors in binding Lactobacillus acidophilus and Bifidobacterium longum cells to amino acid pyrolysates. J. Dairy Sci. 81:1508-1516.
  20. Gilliland, S. E. 1990. Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol. Rev. 87:175-188.
  21. Hosono, A., R. Wardoyo and H. Otani. 1990. Inhibitory effects of lactic acid bacteria from fermented milk on the mutagenecities of volatile nitrosamines. Agric. Biol. Chem. 54:1639-1643.
  22. Usman and A. Hosono. 1998. Desmutagenicity of milk cultured with Lactobacillus acidophilus against mutagenic heated tauco. Food Chem. Toxicol. 36:805-810.
  23. Gilliland, S. E., T. E. Staley and L. J. Bush. 1984. Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J. Dairy Sci. 67:3045-3051.
  24. Gilliland, S. E. and D. K. Walker. 1989. Factor to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans. J. Dairy Sci. 73:905-911.
  25. Nadathur, A. R. and S. J. Gould and A. T. Bakalinsky. 1994. Antimutagenicity of fermented milk. J. Dairy Sci. 77:3287-3295.
  26. Doll, R. and R. Peto. 1981. The causes of cancer: quantitative estimate of avoidable risks of cancer in the United States today. J. Natl. Cancer Inst. 66:1195-1308.
  27. Hosono, A., T. Kashina and T. Kada. 1986a. Antimutagenic properties of lactic acid-cultured milk on chemical and fecal mutagens. J. Dairy Sci. 69:2237-2242.
  28. Hosono, A. and H. Shirai. 1996. Antimutagenicity of milk cultured with lactic acid bacteria against the heated solution of cysteine-glucose. Anim. Sci. Technol. (Jpn.) 67:1076-1081.
  29. Salminen, S. and A. von Wright. 1993. Lactic acid bacteria. Marcel Dekker, Inc., New York, Basel, Hong Kong.