- Volume 16 Issue 1
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Association of a Single Nucleotide Polymorphism in the 5'-Flanking Region of Porcine HSP70.2 with Backfat Thickness in Duroc Breed
- Chen, Ming-Yu ;
- Huang, San-Yuan ;
- Lin, En-Chung ;
- Hseu, Tzong-Hsiung ;
- Lee, Wen-Chuan
- Received : 2002.06.11
- Accepted : 2002.09.10
- Published : 2003.01.01
Higher environmental temperature affects the economic performance of pigs. Heat shock protein 70 has been shown to play an important role in thermoresistance. The purpose of this study was to assess the effect of a single nucleotide polymorphism in the 5'-flanking region of porcine HSP70.2 on growth performance in Taiwanese Duroc. The genotype of this nt 393 polymorphic site could be verified by digestion with Bsa WI restriction enzyme of a PCR product. Pigs with TT and TC genotypes have thinner backfats than those with CC type (p<0.05). The result suggested that the polymorphic Bsa WI site in the 5'flanking region of porcine HSP70.2 may be used as a marker for the early selection of ultrasonic backfat thickness in Duroc pigs.
Pigs Duroc;Heat Shock Protein 70;Growth Performance;Polymorphism
- Holmes, C. W. 1971. Growth and backfat depth of pigs kept at a high temperature. Anim. Prod. 13:521-527. https://doi.org/10.1017/S0003356100010710
- Maak, S., K. Petersen and G. Von Lengerker. 1998. Association of polymorphisms in the porcine HSP70 gene promoter with performance traits. Anim. Genet. 29(Suppl. 1):71(Abstr.).
- Heitman, H. and E. H. Hughes. 1949. The effects of air temperature and relative humidity on the physical wellbeing of swine. J. Anim. Sci. 8:171-181. https://doi.org/10.2527/jas1949.82171x
- Nunes, M., M. Yerles, F. Dezeure, J. Gellin, P. Chardon and M. Vaiman. 1993. Isolation of four HSP70 genes in the pig and localization on Chromosome 7 and 14. Mamm. Genome 4:247-251. https://doi.org/10.1007/BF00417430
- Lindquist, S. and E. A. Craig. 1988. The heat shock proteins. Annu. Rev. Genet. 22:63-77.
- Chyr, S. C. 1980. Swine selection index and direction of pig improvement. J. Chin. Soc. Anim. Sci. 9:55-69.
- Huang, S. Y., H. L. Tsou, M. T. Kan, W. K. Lin and C. S. Chi. 1995. Genetic study on leg weakness and its relationship with economic traits in central tested boars in subtropical area. Livestock Prod. Sci. 44:53-59. https://doi.org/10.1016/0301-6226(95)00063-Q
- Dezeure, F., M. Vaiman and P. Chardon. 1993. Characterization of a polymorphic heat shock protein 70 gene in swine outside the SLA major histocompatibility complex. Biochim. Biophys. Acta 1174:17-26. https://doi.org/10.1016/0167-4781(93)90087-T
- Heitman, H., C. F. Kelly and T. E. Bond. 1958. Ambient air temperature and weight gain in swine. J. Anim. Sci. 17:62-67. https://doi.org/10.2527/jas1958.17162x
- Ruohonen-Lehto, M. K., M. F. Rothschild and R. G. Larson. 1993. Restriction fragment length polymorphisms at the heat shock protein HSP70 gene(s) in pigs. Anim. Genet. 24:67-68. https://doi.org/10.1111/j.1365-2052.1993.tb00923.x
- Schwerin, M., S. Maak, C. Kalbe and R. Fuerbass. 2001. Functional promoter variants of highly conserved inducible hsp70 genes significantly affect stress response. Biochim. Biophys. Acta 1522:108-111. https://doi.org/10.1016/S0167-4781(01)00313-X
- Chen, M. Y., S. Y. Huang, H. L. Tsou, E. C. Lin, Y. H. Kuo, P. C. Yang, T. Y. Huang and W. C. Lee. 2000. Polymorphism in 5'-flanking region of porcine heat shock protein 70.2 gene. Anim. Genet. 31:410-411. https://doi.org/10.1046/j.1365-2052.2000.00682.x
- Holme, D. W. and W. E. Coey. 1967. The effect of environmental temperature and method of feeding on performance and carcass composition of bacon pigs. Anim. Prod. 9:209-218. https://doi.org/10.1017/S0003356100038472
- Peelman, L. J., A. R. Van De Weghe, W. R. Coppieters, A. J. Van Zeveren and Y. H. Bouquet. 1992. Complete nucleotide sequence of a porcine HSP70 gene. Immunogenetics 35:286-289. https://doi.org/10.1007/BF00166836
- SAS Institute, 1989. SAS User's Guide: Statistics. Release 6.3 ed. SAS Institute Inc., Cary, North Carolina, USA.
- Schwerin, M., A. Hagendorf, R. Furbass and F. Teuscher. 1999. The inducible stress protein 70.2 gene-A candidate gene for stress susceptibility in swine. Arch Tierz. 42:61-66.
- Johnston, R. N. and B. L. Kucey. 1988. Competitive inhibition of hsp70 gene expression causes thermosensitivity. Science 243:1551-1554. https://doi.org/10.1126/science.2928791
- Rattink, A. P., D. J. De Koning, M. Faivre, B. Harlizius, J. A. Van Arendonk and M. A. Groenen. 2000. Fine mapping and imprinting analysis for fatness trait QTLs in pigs. Mamm Genome 11:656-61. https://doi.org/10.1007/s003350010117
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