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Effects of Moist Extruded Full-fat Soybeans on Gut Morphology and Mucosal Cell Turnover Time of Weanling Pigs

  • Qiao, Shiyan ;
  • Li, Defa ;
  • Jiang, Jianyang ;
  • Zhou, Hongjie ;
  • Li, Jingsu ;
  • Thacker, P.A.
  • Received : 2002.03.05
  • Accepted : 2002.07.22
  • Published : 2003.01.01

Abstract

Ten barrows, weaned at 28 days (7.2$\pm$0.1 kg BW), were used to evaluate the effects of feeding extruded full-fat soybeans on intestinal morphology and mucosal cell turnover time. All pigs were fed corn-based diets with half of the pigs receiving diets supplemented with 15.5% soybean meal and 3% soybean oil and the remaining pigs fed a diet in which the soybean meal and oil were replaced by 18.5% extruded full-fat soybeans. The pigs were individually placed in $80{\times}150cm$ metabolic cages and fed twice daily an amount approximately equal to their ad libitum intake for a period of 14 days. On day 14, pigs were weighed and then injected intraperitoneally with $^3$H]thymidine ($100{\mu}Ci/kg$ of BW, specific activity 20 Ci/mmol) 6 h after the morning meal. A pig from each treatment was killed 1, 4, 8, 16, or 24 h postinjection and intestinal tissues were collected. Daily gains for pigs fed the soybean diet and extruded full-fat soybean diet were 0.24 and 0.31 kg/day (p=0.05) with feed conversions of 1.58 and 1.39 (p=0.05), respectively. In comparison with pigs fed soybean meal, pigs fed moist extruded full-fat soybeans had a decreased crypt depth in their duodenum and cecum (p<0.1), while the villus height in the mid jejunum and ileum and the total height (villus height plus crypt depth) of the ileum and mid jejunum increased (p<0.05). The villus width in the duodenum and mid jejunum decreased (p<0.05). The number of crypt epithelial cells in the upper jejunum increased but decreased in the ileum, colon and cecum (p<0.05). The number of villus epithelial cells in the ileum and the upper and mid jejunum increased (p<0.05). The time for migration of epithelial cells in the crypt-villus column decreased (p<0.05) in all sites except the upper jejunum, ileum and cecum. The mucosal turnover rate for all intestinal sites except the upper jejunum, colon and cecum decreased (p<0.05). From these data, we conclude that inclusion of moist extruded full-fat soybeans in weanling pig diets can improve the intestinal morphology and slow the migration rate and turnover time of epithelial cells of the small intestine, especially in the mid jejunum compared with soybean meal.

Keywords

Extruded Soybeans;Intestinal Morphology;Cell Turnover Time;Weanling Pigs

References

  1. AOAC. 1990. Official Methods of Analysis. 15th edn. Association of Official Analytical Chemists, Arlington, Virginia.
  2. Kopriwa, B. and C. P. Leblond. 1962. Improvements in the coating technique radioautography. J. Histochem. 10:269-284.
  3. Li, D. F., J. L. Nelssen, P. G. Reddy, F. Blecha, R. D. Klemn and R. D. Goodband. 1991. Interrelationship between hypersensitivity to soybean proteins and growth performance in early-weaned pigs. J. Anim. Sci. 69:4062-4069.
  4. Miller, B. G., T. J. Newby, C. R. Stokes, D. J. Hampson, P. J. Brown and F. J. Bourne. 1984a. The importance of dietary antigen in the cause of post-weaning diarrhoea in pigs. Am. J. Vet. Res. 45:1730-1733.
  5. Miller, B. G., T. J. Newby, C. R. Stokes and F. J. Bourne. 1984b. Influence of diet on post-weaning malabsorption and diarrhoea in the pig. Res. Vet. Sci. 36:187-193
  6. Quastler, H. and F. G. Sherman. 1959. Cell population kinetics in the intestinal epithelium of the mouse. Exp. Cell Res. 17:420-438. https://doi.org/10.1016/0014-4827(59)90063-1
  7. Swick, R. A. 1994. Soybean meal quality. American Soybean Association Technical Bulletin PO11-1994.
  8. Yen, J. T., J. A. Nienaber, D. A. Hill, and W. G. Pond. 1988. Oxygen consumption by whole body and by portal drained organs in swine. J. Anim. Sci. 66(Suppl. 1):331(Abstr.).
  9. Radecki, S. V., P. K. Ku, M. R. Bennink, M. T. Yokoyama and E. R. Miller. 1992. Effect of dietary copper on intestinal mucosa enzyme activity, morphology, and turnover rates in weanling pigs. J. Anim. Sci. 70:1424-1431.
  10. De Schutter, A. C. and J. R. Morris. 1990. Soybeans: Full-fat. In: Non-traditional Feed Sources for Use in Swine Production (Ed. P. A. Thacker and R. N. Kirkwood). Butterworths, Stoneham, Massachusetts. pp. 439-451.
  11. Terlington, W. H., D. S. Pollmann, J. G. Endres and J. A. Coalson. 1990. Soy protein concentrates: An alternative to milk proteins. Feedstuffs, September, p. 16.
  12. Li, D. F., J. L. Nelssen, P. G. Reddy, F. Blecha, J. D. Hancock, G. L. Allee, R. D. Goodband and R. D. Klemn. 1990. Transient hypersensitivity to soybean meal in the early weaned pig. J. Anim. Sci. 68:1790-1799.
  13. Geurin, H. B., G. A. Kesel, W. T. Black, T. B. Hatfield and C. N. Daniels. 1988. Effect of isolated soy protein and whey on replacing dried skim milk in a prestarter for weaned baby pigs. J. Anim. Sci. 66 (Suppl. 1):320 (Abstr.).
  14. Kakade, M. L., J. J. Rackis, J. E. McGhee and G. Puski. 1974. Determination of trypsin inhibitor activity of soy products: A collaborative analysis of an improved procedure. Cereal Chem. 51:376-382.
  15. Friesen, K. G., J. L. Nelssen, R. D. Goodband, K. C. Behnke and L. J. Kats. 1993. The effect of moist extrusion of soy products on growth performance and nutrient utilization in the early-weaned pigs. J. Anim. Sci. 71:2099-2109.
  16. Yangsheng, S. 1993. Methods of Feed Analysis and Feed Quality Determination. Beijing Agricultural University Press, Beijing, China, pp. 260-267.
  17. Imondi, A. R. and F. H. Bird. 1966. The turnover of intestinal epithelium in the chick. Poult. Sci. 45:142-147. https://doi.org/10.3382/ps.0450142
  18. Dunsford, B. R., D. A. Knabe and W. E. Hanesly. 1989. Effect of dietary soybean meal on the microscopic anatomy of the small intestine in the early weaned pigs. J. Anim. Sci. 67:1855-1863.
  19. SAS. 1999. SAS Users Guide, Version 8, SAS Institute Inc., Cary, NC.
  20. Woodworth, J. C., M. D. Tokach, R. D. Goodband, J. L. Nelssen, P. R. O'Quinn, D. A. Knabe and N. W. Said. 2001. Apparent ileal digestibility of amino acids and digestible and metabolizable energy content of dry extruded-expelled soybean meal and its effects on growth performance of pigs. J. Anim. Sci. 79:1280-1287.
  21. Newby, T. J., B. G. Miller, C. R. Stokes, D. J. Hampson and F. J. Bourne. 1984. Local hypersensitivity response to dietary antigens in early weaned pigs. In: Recent Advances in Animal Nutrition (Ed. W. Haresign and D. J. A. Cole). Butterworths, London. pp. 49-59.
  22. Moon, H. W. 1970. Epithelial cell migration in the alimentary mucosa of the suckling pig. Proc. Soc. Exp. Biol. Med. 137:151-156.

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