- Volume 16 Issue 8
The surfactant Tween 80 was evaluated for its ability to influence cumulative gas production, cellulose digestion, and enzyme activities by mixed ruminal microorganisms grown on barley grain or Orchardgrass hay. The addition of Tween 80 at a level of 0.10% significantly (p<0.05) decreased the cumulative gas production rate from both barley grain or Orchardgrass hay substrates. However, 0.05% Tween 80 did not affect gas production rates compared to the control treatment. The addition of 0.05% Tween 80 to cultures growing on barley grain resulted in a significant increase in cellulase (90.01%), xylanase (90.73%) and amylase (487.25%) activities after 30 h incubation. Cultures utilizing Orchardgrass hay had a significant increase in cellulase (124.43%), xylanase (108.86%) and amylase (271.22%) activities after 72 h incubation. These increases in activities were also observed with cultures supplemented with 0.10% Tween 80 throughout all the incubation times tested. These results indicated that the addition of 0.05% Tween 80 could greatly stimulate the release of some of key enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism. Our data indicates potential uses of the surfactant Tween 80 as a feed additive for ruminant animals.
Tween 80, Rumen Microorganisms;Gas Production;Enzyme Activity;Cellulose Digestion;Feed Additives
- Bryant, M. P. and L. A. Burkey. 1953. Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. Dairy Sci. 36:205-217. https://doi.org/10.3168/jds.S0022-0302(53)91482-9
- Demain, A. L. and J. Birnbaum. 1968. Alternation of permeability for the release of metabolites from microbial cells. Curr. Topics Microbiol. Immun. 46:1-25.
- Fedorak, P. M. and S. E. Hrwdey. 1983. A simple apparatus for measuring gas production by methanogenic cultures in serum bottles. Environ. Technol. Lett. 4:425-432. https://doi.org/10.1080/09593338309384228
- Ha, J. K., S. S. Lee, Z. Gao, C. -H Kim, S. W. Kim and K. -J. Cheng. 2001. The effect of saturated fatty acids on cellulose digestion by rumen anaerobic fungus, Neocallimastix frontalis C5-1. Asian-Aust. J. Anim. Sci. 14:941-946.
- Lowe, S. E., G. G. Griffith, A. Milne, M. K. Theodorou and A. P. J. Trinci. 1987a. The life cycle and growth kinetics of an anaerobic rumen fungus. J. Gen. Microbiol. 133:1815-1827.
- Merry, R. J., M. K. Theodorou, M. G. Raurich and M. S. Dhanoa. 1991. Use of head-space gas pressure in batch cultures to assist in determination of nutritive value of silage to rumen bacteria. Presented at a Conference on Forage Conservation Towards 2000. Institute of Grassland and Forage Research, Federal Research Centre of Agriculture, Brauschweig-Volkenrode, Germany.
- SAS. 1996. Statistical analysis systems user's guide: Statistics. SAS Institute. Inc., Cary, NC.
- Lowe, S. E., M. K. Theodorou and A. P. J. Trinci. 1987b. Isolation of anaerobic fungi from saliva and faeces of sheep. J. Gen. Microbiol. 133:1829-1834.
- Theodorou, M. K., D. R. David, B. B. Nielsen, M. I. G. Lawrence, and A. P. J. Trinci. 1995. Determination of growth of anaerobic fungi on soluble and cellulosic substrates using a pressure transducer. Microbiology 141:671-678. https://doi.org/10.1099/13500872-141-3-671
- Lee, S. S., J. K. Ha and K. -J. Cheng. 2001. Effects of LCFA on the gas production, cellulose digestion and celluase activities by rumen anaerobic fungus, Neocallimastix frontalis RE1. Asian-Aust. J. Anim. Sci. 14:1110-1117.
- Lee, S. S., B. H. Ahn, H. S. Kim, C. H. Kim, K. -J. Cheng and J. K. Ha. 2003. Effects of non-ionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performances of lactating cows. Asian-Aust. J. Anim. Sci. 16:104-115.
- Reese, E. T. and A. Maguire. 1969. Surfactants as stimulants of enzyme production by microorganisms. Applied Microbiol. 17:242-245.
- Hulme, M. A. and D. W. Stranks. 1970. Induction and their regulation of production of cellulase by fungi. Nature, London. 226:469-470. https://doi.org/10.1038/226469a0
- Miller, T. L. and M. J. Wolin. 1974. A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl. Microbiol. 27:985-987.
- Hung, B. R., L. Lara, M. A. Patron, N. N. Ugarova, W. Bechstedt, and S. Clappes. 1988. Tween 80 and proteose peptone effect on cellulase production. Acta Biotechnol. 8:461-464. https://doi.org/10.1002/abio.370080515
- Schewale, J. G. and J. C. Sadana. 1978. Cellulase and -glucosidase production by basidiomycetes species. Can. J. Microbiol. 24:1204-1216. https://doi.org/10.1139/m78-195
- Miller, G. L. 1959. Use of dinitrosalycilic acid reagent for determination of reducing sugar. Analytical Chemistry 31:426-428. https://doi.org/10.1021/ac60147a030
- Long, K. and J. S. Knapp. 1991. The effect of Junlon PW110 and Tween 80 on the production of cellulolytic enzymes by Coprinus cinereus. Mycol. Res. 95:1077-1081. https://doi.org/10.1016/S0953-7562(09)80550-9
- Deshpande, M. V., M. C. Srinavasan and S. S. Deshmakh. 1987. Effects of fatty acids on cellulase production by Penicillium funiculosum and its mutants. Biotechnol. Lett. 9:301-304. https://doi.org/10.1007/BF01025792
- Yazdi, M. T., J. R. Woodward, and A. Radford. 1990. The cellulase complex of Neurospora crassa: activity, stability and release. J. Gen. Microbiol. 136:1313-1319. https://doi.org/10.1099/00221287-136-7-1313
- Lee, S. S., C. H. Kim, J. K. Ha, Y. H. Moon, N. J. Choi and K. J. Cheng. 2002. Distribution and activities of hydrolytic enzymes in the rumen compartments of Hereford bulls fed alfalfa based diet. Asian-Aust. J. Anim. Sci. Vol 15: 1725-1731.
- Herrero, M., I. Murray, R. H. Fawcett and J. B. Dent. 1996. Prediction of the in vitro gas production and chemical composition of kikuyu grass by near-infrared reflectance spectroscopy. Anim. Feed Sci. Technol. 60:51-67. https://doi.org/10.1016/0377-8401(95)00924-8
- Hungate, R. E. 1950. The anaerobic mesophyllic cellulolytic bacteria. Bacteriol. Rev. 14:1-49.
- Mountfort, D. O. and R. A. Asher. 1983. Role of catabolite regulatory mechanisms in control of carbohydrate utilization by the rumen anaerobic fungus Neocallimastix frontalis. Appl. Environ. Microbiol. 46:1331-1338.
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