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Changes in Ovarian and Placental 20α-hydroxysteroid Dehydrogenase Activity during the Pregnancy in the Rat

  • Seong, H.H. (Division of Animal Biotechnology National Livestock Research Institute, RDA) ;
  • Min, K.S. (Graduate School of Bio. & Information Technology, Hankyong National University) ;
  • Kang, M.H. (Dept. of Food and Nutrition, Hoseo University) ;
  • Yoon, J.T. (Graduate School of Bio. & Information Technology, Hankyong National University) ;
  • Jin, H.J. (National Livestock Research Institute, RDA) ;
  • Chung, H.J. (National Livestock Research Institute, RDA) ;
  • Chang, W.K. (National Livestock Research Institute, RDA) ;
  • Yun, S.G. (National Livestock Research Institute, RDA) ;
  • Shiota, Kunio (Department of Veterinary Biochemistry, The University of Tokyo)
  • Received : 2002.09.02
  • Accepted : 2002.12.31
  • Published : 2003.03.01

Abstract

The enzyme $20{\alpha}$-hydroxysteroid dehydrogenase ($20{\alpha}$-HSD) catabolizes progesterone to $20{\alpha}$-dihydroprogesterone ($20{\alpha}$-OHP), and is appeared in rat corpora luteal and placenta. A polled samples of 10-15 placental and ovarian tissues collected from each individual rat were subjected to measurement of $20{\alpha}$-HSD activity. A $20{\alpha}$-HSD activity in the cytosol fraction was based on the generation of NADPH. In this study, it is designed to study cytosolic $20{\alpha}$-HSD activity in rat ovarian and placenta during pregnancy, and its relationship to embryonic mortality. It was found that from days 5 to 18 of pregnancy the $20{\alpha}$-HSD activities steady by decreased but at parturition time rapidly increased in ovary. On the other hand, placental cytosolic $20{\alpha}$-HSD activities were high detected from days 8 to 10 of pregnancy, not detectable from days 11 to 20 of pregnancy, but again very high at the time of parturition. Analysis of DEAE column chromatography revealed that two different types of $20{\alpha}$-HSD (HSD-1 and HSD-2) were found with similar activity in the placental cytosol on day 10 of pregnancy. The number of fetuses on day 10 of pregnancy was 15.4 and decreased significantly to 12.9 on day 12. The results suggested that expression of $20{\alpha}$-HSD in the placental tissues seems to be related the number of fetal survived in the specific time (days 11 and 12) which spontaneous fetal loss occurs.

Keywords

$20{\alpha}$-HSD Activity;Progesterone;$20{\alpha}$-dihydroprogesterone;Rat Ovary

References

  1. Nakajin, S., Y. Kawal, S. Ohno and M. Shinoda. 1989. Purification and characterization of pig adrenal $20{\alpha}$-hydroxysteroid dehydro-genage. J. Steroid Biochem. 33:1181-1198. https://doi.org/10.1016/0022-4731(89)90428-7
  2. Pollard, J. W., A. Bartocci, R. Arceci, A. Orlofsky, M. B. Ladner and E. R. Stanley. 1987. Apparent role of the macrophage growth factor, CSF-1, in placental development. Nature 330:484-486. https://doi.org/10.1038/330484a0
  3. Albarracin, C. T., T. G. Parmer, W. R. Duan, S. E. Nelson and G. Gibori. 1994. Identification of a major prolactin-regulated protein as 20 alpha-hydroxysteroid dehydrogenase: coordinate regulation of its activity, protein content, and messenger ribonucleic acid expression. Endocrinology 134:2453-2460. https://doi.org/10.1210/en.134.6.2453
  4. Matsuyama, S., K. Shiota and M. Takahashi. 1990. Possible role of transforming growth factor-$\beta$ as a mediator of lucotropic action of prolactin in the rat luteal cell cultures. Endocrinology 127:1561-1567. https://doi.org/10.1210/endo-127-4-1561
  5. Olofsson. J., E. Norjavaara and G. Selstam. 1992. Synthesis of prostaglandin F2 alpha, E2 and prostacyclin in isolated corpora lutea of adult pseudopregnant rats throughout the luteal lifespan. Prostaglandins Leukot Essent Fatty Acids 46:151-161. https://doi.org/10.1016/0952-3278(92)90222-5
  6. Csapo, A. I and W. G. Wiest. 1969.An examination of the quantitative relationship between progesterone and the maintenance of pregnancy. Endocrinology 85; 735-746 https://doi.org/10.1210/endo-85-4-735
  7. Pineda, J.A., M. F. Salinas and J. C. Warren. 1985. Purification and characterization of $20{\alpha}$-hydroxysteroid dehydro-genase from bull testis. J. Steroid Biochem. 23:1001-1006. https://doi.org/10.1016/0022-4731(85)90059-7
  8. Seong, H. H., K. Shiota, K. Noda, A. Ogure. T. Asano and M. Takahashi. 1992. Expression of activites of two $20{\alpha}$-hydroxysteroid dehydrogenase in rat corpora lutea. J. Reprod. Fertil. 96:573-580. https://doi.org/10.1530/jrf.0.0960573
  9. Kuhn, N. J and M. S. Briley. 1970. The roles of pregn- 5-ene-$3{\beta}$, $20{\alpha}$-atiol and $20{\alpha}$-hydroxysteroid dehydro-genase in the control of progesterone synthesis preceding parturition and lactogenesis in the rat. Biochemical J. 117:193-201. https://doi.org/10.1042/bj1170193
  10. Bladford, M. M. 1976. A rapid and sensitive method for quantitation of microgram quantities utilizing the principle of protein-dye binding. Annu. Biochem. 72:248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  11. Murakami, N., M. Takahashi, Y. Suzuki and K. Homma. 1982. Responsiveness of despersed rat luteal cells to luteinizing hormone and PRL during the estrous cycle and early pseudopregnancy. Endocrinology 111:500:508.
  12. Miura, R., K. Shiota, K. Noda, S. Yagi, T. Ogawa and M. Tahahashi. 1994. Molecular cloning of cDNA for rat ovarian 20 alpha-hydroxysteroid dehydrogenase (HSD1). Biochem. J. 299:561-567. https://doi.org/10.1042/bj2990561
  13. Noda, K., H. H. Seong, K. Shiota and M. Takahashi. 1992. Changes of two kinds of $20{\alpha}$- hydroxysteroid dehydrogenase during luteal phase in the rat. J. Reprod. Develop. 38:49-53. https://doi.org/10.1262/jrd.38.49
  14. Shiota, K., H. H. Seong, K. Noda, N. Hattori, A. Ikeda, A. Ogura, S-I. Itagaki, M. Takahashi and T. Ogawa. 1993. $20{\alpha}$-hydroxysteroid dehydrogenase activity in rat placenta. Endocrine J. 40:673-681. https://doi.org/10.1507/endocrj.40.673
  15. Shikita. M., H. Inano and B. Tamaoki. 1967. Further studies in $20{\alpha}$-hydroxysteroid dehydro genase of rat testes. Biochemistry 6:1760-1764. https://doi.org/10.1021/bi00858a026
  16. Clarke, C. L and R. L. Sutherland. 1990. Progesterone regulation of cellular proliferation. Endocrine Rev. 14:266-301.
  17. Noda, K., K. shiota and M. Takahashi. 1991. Purification and characterization of rat ovarian $20{\alpha}$-hydroxysteroid dehydrogenase. Biochem. Biophys. Acta. 1079:112-118. https://doi.org/10.1016/0167-4838(91)90031-T
  18. Nancarrow, C. D. M. A. Sharaf and F. Sweet. 1981. Purification of $20{\alpha}$ hydroxysteroid oxidoreductase from bovine fetal erythrocytes. Steroids 37:530-553. https://doi.org/10.1016/S0039-128X(81)90370-6
  19. Matsuda, J., K. Noda, K. Shiota and M. Takahashi. 1990. Participation of ovarian $20{\alpha}$-hydroxysteroid dehydrogenase in luteotrophic and luteolytic processes during rat pseudopregnancy. J. Reprod. Fertil. 88: 467 - 478. https://doi.org/10.1530/jrf.0.0880467
  20. Mori, M. and W. G. Wiest. 1979. Purification of $20{\alpha}$- hydroxysteroid dehydro-genase by affinity chromatography. J. Steroid Biochem. 14:1443-1449.
  21. Fukuda, T., K. Hirato, T. Yanaihara and T. Nakayama. 1986. Microsonal $20{\alpha}$-atiol and $20{\alpha}$-hydroxysteroid dehydrogenase activity for progesterone in human placenta. Endocrinol Japon 33:361-368. https://doi.org/10.1210/endo-33-6-361
  22. Shiota, K., N. Sasaki, N. Hattori, R. Miura, T. Hasegawa, J-X. Guang, K. Noda and M. Takahashi. 1991. Cytosolic $20{\alpha}$-hydroxysteroid dehydrogenase activity in spontaneous Neoplasms in the dog and cat. J. Vet. Med. Sci. 53:549-552. https://doi.org/10.1292/jvms.53.549
  23. Sharaf, M. and F. Sweet. 1982. Dual activity at an enzyme active site: $3{\beta}$, $20{\alpha}$- hydroxysteroid oxidoreductase from fetal blood. Biochemistry 21:4615-4620. https://doi.org/10.1021/bi00262a016

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