- Volume 16 Issue 5
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Effects of Surfactant Tween 80 on Forage Degradability and Microbial Growth on the In vitro Rumen Mixed and Pure Cultures
- Goto, M. (Faculty of Bioresources, Mie University) ;
- Bae, H. (Kyonggi Provincial Government) ;
- Lee, S.S. (National livestock Institute) ;
- Yahaya, M.S. (Faculty of Bioresources, Mie University) ;
- Karita, S. (Faculty of Bioresources, Mie University) ;
- Wanjae, K. (Faculty of Bioresources, Mie University) ;
- Cheng, K.J. (Department of Microbiology, University of British Columbia)
- Received : 2002.09.02
- Accepted : 2003.01.08
- Published : 2003.05.01
Effect of a surfactant Tween 80 on the bacterial growth in the rumen was examined on the in vitro pure cultures of Streptococcus bovis, Selenomonas ruminantium, Butyrivibrio fibrisolvens, Prevotella ruminicola, Megasphaera elsidenni, Fibrobacta succinogenes, Ruminanococcus albus and Ruminococcus flavefaciens. Dry matter degradability (DMD), concentrations and compositions of volatile fatty acids (VFA), and the most probable number (MPN) of cellulolytic bacteria and total number of bacteria in the presence of Tween 80 were also examined on the in vitro rumen mixed culture either with barley grain or orchardgrass hay. The growth of S. bovis, S. ruminantium, B. fibrisolvens, P. ruminicola, M. elsidenni and F. succinogenes were significantly higher (p<0.05) at over 0.05% concentrations of Tween 80 than those of the control cultures, while was not changed with R. albus and R. flavefaciens. With rumen mixed culture the DMD of barley grain and orchardgrass hay was significantly higher (p<0.05) at a 0.2% concentration of Tween 80 than the control, being reflected in the significantly higher (p<0.05) VFA production (mmol
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