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Effect of Diet on Enzyme Profile, Biochemical Changes and In sacco Degradability of Feeds in the Rumen of Buffalo

  • Kamra, D.N. (Microbiology Section, Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Saha, Sudipto (Microbiology Section, Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Bhatt, Neeru (Microbiology Section, Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Chaudhary, L. C. (Microbiology Section, Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Agarwal, Neeta (Microbiology Section, Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute)
  • Received : 2002.07.06
  • Accepted : 2002.10.09
  • Published : 2003.03.01

Abstract

Four rumen fistulated Murrah buffaloes were used to study the effect of four diets differing in roughage to concentrate ratio on rumen biochemical changes, microbial enzyme profile and in sacco degradability of feed in a $4{\times}4$ Latin Square design. The animals were fed four diets consisting of 80:20, 70:30, 60:40 and 50:50 ratios of wheat straw and concentrate mixtures, respectively. Wheat straw and concentrate mixture were mixed with water (0.6 l/kg feed) and complete feed mixture was offered to the animals at 8:00 h and 16:00 h in two equal parts. The variation in pH of rumen liquor (difference of maximum and minimum during 0-8 h post feeding) increased with increasing level of concentrate mixture in the diet. There was no effect of diet composition on volatile fatty acids, total nitrogen and trichloro-acetic acid precipitable nitrogen in the rumen liquor, but ammonia nitrogen increased with increasing level of concentrate mixture in the ration. Major portions of all fibre degrading enzymes were present in the particulate material (PM) of the rumen contents, but protease was absent in PM fraction. The activities of micro-crystalline cellulase, acetyl esterase and protease increased with increase in the level of concentrate mixture, but the activities of other enzymes (carboxymethylcellulase, filter paper degrading activity, xylanase, $\beta$-glucosidase and $\beta$-xylosidase) were not affected. The in sacco degradability and effective degradability of feeds increased with increasing level of concentrate mixture in the ration.

Keywords

Rumen Enzymes;Buffalo;Enzyme Profile;ISDMD;Diet Effect;Rumen Fermentation;Roughage Effect;Cellulase;Xylanase;Acetyl Esterase

Acknowledgement

Supported by : National Agricultural Technology

References

  1. Akin, D. E. 1979. Microscopic evaluation of fibre digestion by rumen microorganisms : A review. J. Anim. Sci. 48:701-710. https://doi.org/10.2527/jas1979.483701x
  2. Borneman, W. S., L. G. Ljungdahl, R. D. Hartley and D. E. Akin. 1991. Isolation and characterization of p-coumaroyl esterase from the anaerobic fungus Neocallimastix strain MC-2. Appl. Environ. Microbiol. 57:2337-2344.
  3. Lowry, O. H., N. J. Rosenbrough, A. L. Farr and R. C. Randall. 1951. Protein measurement with the Folin-phenol reagent. J. Biol. Chem. 183:265.
  4. McSweeney, C. S., B. P. Dalrymple, K. S. Gobius, P. M. Kennedy, D. O. Krause, R. I. Mackie and G. P. Xue. 1999. Theapplication of rumen biotechnology to improve the nutritive value of fibrous feedstuffs : pre- and post-ingestion. Livestock Prod. Sci. 59:265-283. https://doi.org/10.1016/S0301-6226(99)00032-9
  5. Miller, G. L. 1959. Modified DNS method for reducing sugars. Anal. Chem. 31:426-428 https://doi.org/10.1021/ac60147a030
  6. Orskov, E. R. and I. McDonald. 1979. The estimation of protein degradability in rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. (Camb.) 92:499-503. https://doi.org/10.1017/S0021859600063048
  7. Sahu, N. P. and D. N. Kamra. 2002. Microbial eco-system of the gastro-intestinal tract of wild herbivorous animals. J. Appl. Anim. Res. 21:207-230. https://doi.org/10.1080/09712119.2002.9706370
  8. Silva, A. T., R. J. Wallace and E. R. Orskov. 1987. Use of particle bound microbial activity to predict the rate and extent of fibre degradation in the rumen. Br. J. Nutr. 57:407-415. https://doi.org/10.1079/BJN19870048
  9. Tanaka, M., M. Taniguchi, R. Matsuno and T. Kamikubo. 1988. Cellulases from Eupenicillium javanicum. Methods in Enzymology 160:251-259. https://doi.org/10.1016/0076-6879(88)60127-3
  10. Van der Linden, Y., N. O. van Gylswyk and H. M. Schwatz. 1984. Influence of supplementation of corn stover with corn grain on the fibrolytic bacteria in the rumen of sheep and their relation to the intake and digestion of fibre. J. Anim. Sci. 59:772-783. https://doi.org/10.2527/jas1984.593772x
  11. Cottyn, B. G. and C. V. Boucque. 1968. Rapid method for the gaschromatographic determination of volatile fatty acids in rumen fluid. J. Agric. Food Chem. 16:105-107. https://doi.org/10.1021/jf60155a002
  12. Hobson, P. N. and C. S. Stewart. 1997. The Rumen Microbial Ecosystem. Blackie Academic & Professional, London.
  13. Ushida, K., H. Matsui, Y. Fujino and J. K. Ha. 1997. Role and potential of ruminal fungi in fibre digestion. A review. Asian-Aust. J. Anim. Sci. 10: 541-550.
  14. Krause, D. O., R. J. Bunch, W. J. M. Smith and C. S. McSweeney. 1998. Diversity of Ruminococcus strains : a survey of genetic polymorphism and plant digestibility. J. Appl. Microbiol. 86: 487-495.
  15. Brock, F. M., C. L. Forsberg and J. G. Buchanan-Smith. 1982. Proteolytic activity of rumen microorganisms and effects of proteinase inhibitors. Appl. Environ. Microbiol. 44:561-569.
  16. Williams, A. G., S. E. Withers and C. G. Orpin. 1994. Effect of the carbohydrate growth substrate on polysaccharolytic enzyme formation by anaerobic fungi isolated from the foregut and hindgut of non ruminant herbivores and the forestomach of ruminants. Letters Appl. Microbiol. 18:147-151. https://doi.org/10.1111/j.1472-765X.1994.tb00830.x
  17. Russell, J. B. and D. B. Dombrowski. 1980. Effects of pH on the efficiency of growth by pure cultures of rumen bacteria in continuous culture. Appl. Environ. Microbiol. 34:604.
  18. Agarwal, N., I. Agarwal, D. N. Kamra and L. C. Chaudhary. 2000. Diurnal variations in the activities of hydrolytic enzymes in different fractions of rumen liquor of Murrah buffalo. J. Appl. Anim. Res. 18:73-80. https://doi.org/10.1080/09712119.2000.9706325
  19. Lee, S. S., K. J. Shin, W. Y. Kim, J. K. Ha and I. K. Han. 1999. The rumen eco-system as a fountain source of novel enzymes. Asian-Aust. J. Anim. Sci. 12:988-1001.
  20. Fujino, Y. and K. Ushida. 1999. Plant cell wall degradation and glycanase activity of the rumen anaerobic fungus Neocallimastix frontalis MCH3 grown on various forages. Asian-Aus. J. Anim. Sci. 12:752-757. https://doi.org/10.5713/ajas.1999.752
  21. Hiltner, P. and B. A. Dehority. 1983. Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria. Appl. Environ. Microbiol. 46:642.
  22. Shewale, J. G. and J. C. Sadana. 1978. Cellulase and $\beta$- glucosidase production by a basidomycete species. Can. J. Microbiol. 24:1204-1216.
  23. Snedecor, G. W. and W. G. Cochran. 1967. In: Statistical Methods 6th ed. Oxford IBH Publishing, Calcutta.
  24. Morris, J. and O. J. Cole. 1987. Relationship between cellulolytic activity and adhesion to cellulose in Ruminococcus albus. J. Gen. Microbiol. 133: 53-69.
  25. Singh, B., T. K. Bhat and B. Singh. 2001. Exploiting gastrointestinal microbes for livestock and industrial development: A review. Asian-Aust. J. Anim. Sci. 14:567-586.
  26. Hungate, R. E. 1966. Rumen and its Microbes, Academic Press, New York.
  27. AOAC. 1988. Official Methods of Analysis, 16th edn., Association of Official Analytical Chemists, Washington, DC.
  28. Huhtanen, P. and H. Khalili. 1992. The effect of sucrose supplements on particle associated carboxymethylcellulase and xylanase activities in cattle given grass silage based diet. Brit. J. Nutr. 67:245-255. https://doi.org/10.1079/BJN19920028
  29. Agarwal, N., N. Kewalramani, D. N. Kamra, D. K. Agrawal and K. Nath. 1991. Hydrolytic enzymes of buffalo rumen: Comparison of cell free rumen fluid, bacterial and protozoal fractions. Buffalo J. 7:203-207.
  30. Ha, J. K., S. S. Lee, M. Goto, Y.H. Moon and K. J. Cheng. 2002. Influence of tween 80 on the enzyme distribution in rumen liquor and on the growth of rumen bacteria and fungi. J. Appl. Anim. Res. 21:129-143. https://doi.org/10.1080/09712119.2002.9706366
  31. Borneman, W. S., L. G. Ljungdahl, R. D. Hartley and D. E. Akin. 1992. Purification and partial characterization of two feruloyl esterases from the anaerobic fungus Neocallimastix strain MC-2. Appl. Environ. Microbiol. 58:3762-3766.
  32. Hristov, A. N., T. A. McAllister and K. J. Cheng. 1999. Effect of diet, digesta processing, freezing and extraction procedure on some polysaccharide degrading activities of ruminal contents. Can. J. Anim. Sci. 79:73-81. https://doi.org/10.4141/A98-056
  33. Williams, A. G., S. E. Withers and N. H. Strachan. 1989. Postprandial variations in the activity of polysaccharide degrading enzymes in microbial populations from the digesta solids and liquor fractions of rumen contents. J. Appl. Bacteriol. 66:15-26. https://doi.org/10.1111/j.1365-2672.1989.tb02449.x
  34. Huggins, C. and J. Lapides. 1947. Acetyl esters of p-nitrophenol as substrate for the colorimetric determination of esterase. J. Biol. Chem. 170:467-482.

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