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Anti-idiotypic Antibodies against Bovine Growth Hormone

  • Verma, N.K. ;
  • Sodhi, R. ;
  • Rajput, Y.S.
  • Received : 2002.06.21
  • Accepted : 2002.11.21
  • Published : 2003.05.01

Abstract

Anti-antibodies against three mouse monoclonal antibodies viz. IIB5D6, VIA6E8 and VIC1F9 (specific to bovine growth hormone) in rabbits have been generated and characterized. Ammonium sulfate fractionated and affinity-purified monoclonal antibodies were used for producing anti-antibodies. The generated anti-antibodies were against common as well as uncommon antigenic determinants present in mouse monoclonal antibodies. The raised anti-antibodies replaced [$I^125$ ]bGH bound to goat liver microsomes indicating production of anti-idiotypic antibodies against bovine growth hormone. These antibodies can have profound implications in vivo in lactating bovines for enhancing milk yield.

Keywords

Anti-idiotypic Antibodies;Bovine Growth Hormone;Bovine Somatotropin;Receptor Assay;Antibody Purification

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Cited by

  1. Development and Characterization of a Novel Anti-idiotypic Monoclonal Antibody to Growth Hormone, Which Can Mimic Physiological Functions of Growth Hormone in Primary Porcine Hepatocytes vol.28, pp.4, 2015, https://doi.org/10.5713/ajas.14.0600

Acknowledgement

Supported by : USDA