L-Arginine Ameliorates Kidney Function and Urinary Bladder Sensitivity in Experimentally-induced Renal Dysfunction in Rats

  • Mansour, Mahmoud A. (Department of Pharmacology, College of Pharmacy, King Saud University) ;
  • Al-Shabanah, Othman A. (Department of Pharmacology, College of Pharmacy, King Saud University) ;
  • El-Khashef, Hassan A. (Department of Pharmacology, College of Pharmacy, King Saud University)
  • Received : 2002.12.25
  • Accepted : 2003.02.25
  • Published : 2003.07.31


Effects of L-arginine and NG-nitro-L-arginine methyl ester (L-NAME) on the renal dysfunction that is induced by cisplatin (CDDP) were investigated. A single dose of CDDP (7.5 mg/kg i.p.) induced renotoxicity, which was manifested by increasing the sensitivity of isolated urinary bladder rings to acetylcholine (ACh), together with a significant elevation of serum urea and creatinine, and a severe decrease in serum albumin. Moreover, renal dysfunction was further confirmed by a significant decrease of enzyme activities, such as glutathione peroxidase, GSH-Px (E.C, catalase (E.C, as well as a significant increase in lipid peroxides that were measured as malondialdhyde (MDA) in kidney tissue homogenates. The administration of L-arginine (70 mg/kg/d p.o in drinking water 5 d before and 5 d after the CDDP injection) significantly ameliorated the renotoxic effects of CDDP, as judged by restoring the normal responses of isolated bladder rings to Ach, and also by an improvement in a range of renal function indices, which included serum urea and creatinine concentrations and kidney weight. In addition, L-arginine prevents the rise of MDA, as well as a reduction of GSH-Px and catalase activities in kidney tissues homogenates. On the other hand, the administration of L-NAME (4 mg/kg/d p.o) resulted in no protection against renal dysfunction that was induced by CDDP treatment. The findings of this study suggest that L-arginine can attenuate kidney injury that is produced by CDDP treatment. In addition, L-arginine may be a beneficial remedy for CDDP-induced renal toxicity, and could be used to improve the therapeutic index of CDDP.


Cisplatin;Rat;Creatinine;Kidney;L-Arginine;Oxidative stress;Urea


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