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Possibility of Using DNA Chip Technology for Diagnosis of Human Papillomavirus

  • Liu, Cui-Hua (Institute of Molecular Biology, First Uniform Medical University) ;
  • Ma, Wen-Li (Institute of Molecular Biology, First Uniform Medical University) ;
  • Shi, Rong (Institute of Molecular Biology, First Uniform Medical University) ;
  • Ou, Yang-Qian (Institute of Molecular Biology, First Uniform Medical University) ;
  • Zhang, Bao (Institute of Molecular Biology, First Uniform Medical University) ;
  • Zheng, Wen-Ling (Institute of Molecular Oncology, Guangzhou General Hospital of Guangzhou Command)
  • Received : 2002.12.10
  • Accepted : 2003.02.14
  • Published : 2003.07.31

Abstract

To explore the application of DNA chip technology for the detection and typing of Human Papillomavirus (HPV), the HPV6, 11, 16 and 18 gene fragments were isolated and printed onto aminosilane-coated glass slides by a PixSys 5500 microarrayer as probes to prepare the HPV gene chips. HPV samples, after being labeled with fluorescent dye by restriction display PCR (RD-PCR) technology, were hybridized with the microarray, which was followed by scanning and analysis. The experimental condition for preparing the HPV gene chips was investigated, and the possibility of HPV genotyping using gene chips was discussed. The technique that was established in this study for preparing HPV gene chips is practical. The results of the present study demonstrated the versatility and inspiring prospect of using this technology to detect and genotype HPV.

Keywords

DNA chip technology;Fluorescence labeling;Human papillomavirus (HPV);Random-priming;Restriction display PCR (RD-PCR)

Acknowledgement

Supported by : National Natural Sciences Foundation of China

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