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Production of Superoxide Dismutase by Deinococcus radiophilus

  • Yun, Young-Sun (Division of Life Sciences and Research Institute for Biotechnology, Chungbuk National University) ;
  • Lee, Young-Nam (Division of Life Sciences and Research Institute for Biotechnology, Chungbuk National University)
  • Received : 2002.11.20
  • Accepted : 2002.12.26
  • Published : 2003.05.31

Abstract

The production of superoxide dismutase (SOD) varied in Deinococcus radiophilus, the UV resistant bacterium, depending upon different phases of growth, UV irradiation, and superoxide treatment. A gradual increase in total SOD activity occurred up to the stationary phases. The electrophoretic resolution of the SOD in cell extracts of D. radiophilus at each growth phase revealed the occurrence of MnSOD throughout the growth phases. The SOD profiles of D. radiophilus at the exponential phase received oxidative stress by the potassium superoxide treatment or UV irradiation also revealed the occurrence of a single SOD. However, these treatments caused an increase in SOD activity. The data strongly suggest that D. radiophilus has only one species of SOD as a constitutive enzyme, which seems to be a membrane-associated protein.

Acknowledgement

Supported by : Korea Science and Engineering Foundation

References

  1. Agostini, H. J., Carroll, J. D. and Minton. K. W. (1996) Identification and characterization of uvrA, a DNA repair gene of Deinococcus radiodurans. J. Bacteriol. 178, 759-765.
  2. Battista, J. R. (1997) Against all odds: The survival strategies of Deinococcus radiodurans. Ann. Rev. Microbiol. 51, 203-224. https://doi.org/10.1146/annurev.micro.51.1.203
  3. Bauche, C. and Laval, J. (1999) Repair of oxidized bases in the extremely radiation-resistant bacterium Deinococcus radiodurans. J. Bacteriol. 181, 262-269.
  4. Beauchamp, C. and Fridovich, I. (1971) Superoxide dismutase: improved assays and assay applicable to acrylamidegels. Anal. Chem. 44, 276-287.
  5. Cho, Y. and Song, K. B. (2000) Effect of $\Upsilon$-radiation on the molecular properties of bovine serum albumin and b-lactoglobulin. J. Biochem. Mol. Biol. 33, 133-137.
  6. Cho, Y. -W., Park, E. -H., Ahn, K. -S., Kim, D. and Lim, C. -J. (2001) Growth-dependent variations in antioxidant and redox enzymes activities of Schizosaccharomyces pombe. J. Biochem. Mol. Biol. 34, 278-283.
  7. Chou, F. I. and Tan, S. T. (1990) Manganese (II) induces cell division and increases in superoxide dismutase and catalase activities in an aging Deinococcal culture. J. Bacteriol. 172, 2029-2035.
  8. Daly, M. J., Ouyang, L., Fuchs, P. and Minton, K. W. (1994) In vivo damage and RecA-dependent repair of plasmid and chromosomal DNA in the radiation-resistant bacterium Deinococcus radiodurans. J. Bacteriol. 176, 3508-3517.
  9. Evans, D. M. and Moseley, B. E. (1983) Roles of the uvs C, uvsD, uvsE and mtcA genes in the two pyrimidine dimer excision repair pathways of Deinococcus radiodurans. J. Bacteriol. 156, 576-583.
  10. Farr, S. B. and Kogoma, T. (1991) Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiol. Rev. 55, 561-585.
  11. Fridovich, I. (1995) Superoxide radical and superoxide dismutase. Ann. Rev. Biochem. 64, 97-112. https://doi.org/10.1146/annurev.bi.64.070195.000525
  12. Gersten, D. M. (1996) Gel Electrophoresis: Proteins, essential techniques, D. Rickwood (ed). Wiley & Sons, West Sussex, UK.
  13. Gregory, E. M., Yost Jr., F. J. and Fridovich, I. (1973) Superoxide dismutase of Escherichia coli intracellular localization and function. J. Bacteriol. 115, 987-991.
  14. Gutman, P. D., Carroll, J. D., Masters, C. I. and Minton, K. W. (1994) Sequencing, targeted mutagenesis and expression of recA gene required for the extreme radioresistance of Deinococcus radiodurans. Gene 141, 31-37. https://doi.org/10.1016/0378-1119(94)90124-4
  15. Halliwell, B. and Gutteridge, J. M. C. (1999) Free Radicals in Biology and Medicine (3rd ed.), pp. 105-350. Oxford Univ. Press, Oxford, UK.
  16. Kim, E. -J., Kim, H. -P., Hah, Y. C. and Roe, J. -H. (1996) Differential expression of superoxide dismutases containing Ni and Fe/Zn in Streptomyces coelicolor. Eur. J. Biochem. 241, 178-185. https://doi.org/10.1111/j.1432-1033.1996.0178t.x
  17. Kim, J., Sharma. A. K., Abbott, S. N., Wood, E. A., Dwyer, D. W., Jambura, A., Minton, K. W., Inman, R. B., Daly, M. J. and Cox. M. M. (2002) RecA protein from the extremely radioresistant bacterium Deinococeus radiorurans: Expression, purification and characterization. J. Bacteriol. 184, 1649-1660. https://doi.org/10.1128/JB.184.6.1649-1660.2002
  18. Lee, I. J. and Lee, Y. N. (1995) Purification and characterization of catalase-3 of Deinococcus radiophilus ATCC 27603. J. Microbiol. 33, 239-243.
  19. Li, T., Huang, X., Zhou, R., Liu, Y., Li, B., Nomura, C. and Zhao, J. (2002) Differential expression and localization of Mn and Fe superoxide dismutase in the heterocystous Cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol. 184, 5096-5103. https://doi.org/10.1128/JB.184.18.5096-5103.2002
  20. Lowry, O. H., Rosebrough, N. J., Farr, A. C. and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
  21. Madigan, M. T., Martinko, J. M. and Parker, J. (2000) Brock Biology of Microorganisms. 9th ed., P. 540. Prentice Hall, Upper Saddle River, NY, USA.
  22. Makarova, K. S., Aravind, L., Wolf, Y. I., Tatusov, R. L., Minton, K. W., Koomin, E. V. and Daly, M. J. (2001) Genome of the extremely radiation resistant bacteria, Deinococcus radiodurans viewed from the perspective of comparative genomics. Microbiol. Mol. Biol. Rev. 65, 44-79. https://doi.org/10.1128/MMBR.65.1.44-79.2001
  23. Marklund, S. and Marklund, G. (1974) Assay of SOD by pyrogallol autotoxidation. Eur. J. Biochem. 47, 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  24. Mueller, D. J., Baumeister, W. and Engel, W. A. (1996) Conformational change of the hexagonally packed intermediate layer of Deinococcus ratiiorurans monitored by atomic force microscopy. J. Bacteriol. 178, 3025-3030.
  25. Murray, R. G. E. (1986). Family II, Deinococcaceae; in Bergeys Manual of Systematic Baeteriology, Buchanan, R. E. and Gibbons, N. E. (eds.), pp. 1035-43. The Williams and Wilkins Co., Baltimore, USA.
  26. Narumi. I., Cherdchu, K., Kikuchi, S., Kitayama, T. and Watanabe, H. (1997) The Deinococcus radiodurans uvrA gene: identification of mutation sites of two mitomycin-sensitive strains and the first discovery of insertion sequence element from deinobacteria. Gene 198, 115-126. https://doi.org/10.1016/S0378-1119(97)00301-6
  27. Dh, K. A. and Lee, Y. N. (1998) Purification and characterization of catalase-2 of Deinococcus radiophilus ATCC 27603. J. Biochem. Mol. Biol. 31, 144-148.
  28. Park, Y-B. and Han, Y-H. (1997) Increased activities of SOD and catalases on aerobic growth in Arcobacter nitrofigilis. J. Microbiol. 35, 239-240.
  29. Schnell, S. and Steinman, H. M. (1995) Function and stationary-phase induction of peri plasmic copper-zinc superoxide dismutase and catalase/peroxidase in Caulohacter crescentus. J. Bacteriol. 177, 5924-5929.
  30. Soung, N. K. and Lee, Y. N. (2000) Iso-catalase profiles of Deinococcus spp. J. Biochem. Mol. Biol. 33, 412-416.
  31. St. John, G. and Steinman, H. M. (1996) Periplasmic copper-zinc superoxide dismutase of Legionella pneumoniae: role in stationary phase survival. J. Bacteriol. 178, 1578-1584.
  32. Storz, G. and Zheng, M. (2000) Oxidative stress; in Bacterial Stress Responses, Storz, G. and Hengge-Aronis, R. (eds.) pp. 47-59, ASM Press, Washington D.C., USA.
  33. Tyrrell, R. M. (1991) UVA (320-380 nm) radiation as an oxidative stress; in Oxidative stress. oxidants and antioxidants, Sies, H. (ed.), pp. 57-83, Academic Press, London, UK.
  34. White, O., Eisen, J. A., Heidelberg, J. F., Hickey, E. K. and 28 persons. (1999) Gemone sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science 286, 1571-1577. https://doi.org/10.1126/science.286.5444.1571
  35. Youn, H. D., Kim, E. J., Hah, Y. C. and Kang, S. O. (1996) A novel nickel-containing superoxide dismutase from Streptomyces spp. Biochem. J. 318, 889-896.
  36. Yun, E. J. and Lee, Y. N. (2000) Production of two different catalase-peroxidases by Deinococcus radiophilus. FEMS Microbiol. Lett. 184, 155-159. https://doi.org/10.1111/j.1574-6968.2000.tb09007.x
  37. Yun, Y. S. and Lee, Y. N. (2001) Superoxide dismutase profiles in the mesophilic Deinococcus species. J. Microbiol. 39, 232-235.

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