The Effect of Acute Sinusitis on the Ultrastructure and Sialic Acid Distribution on the Sinus Mucosa Cell Surface of the Rabbit

실험토끼 상악동염이 상피세포 표면의 미세구조변화와 Sialic acid의 분포에 미치는 영향

  • Kim, Soo-Jin (Department of Biology, College of Natural Sciences, Hallym University) ;
  • Lee, Eun-Jung (Department of Biology, College of Natural Sciences, Hallym University)
  • 김수진 (한림대학교 자연과학대학 생물학과) ;
  • 이은정 (한림대학교 자연과학대학 생물학과)
  • Published : 2002.06.01

Abstract

Experimatal maxillary sinusitis was induced in New Zealand white rabbits by blocking the maxillary sinus ostium. The distribution of lectin receptors was explored in the mucosa with induced maxillary sinusitis using colloidal gold label complex with lectin WGA purified from wheat germ (Triticum vulgaris). The lectin WGA gold complex, shown to recognize GlcNac (N-acetylglucosamine) and NeuNAc (N-acetylneuraminic acid) regions, was applied to detect binding sites in Lowicryl HM 20 sections and viewed under the electron microscope. An increased height of the cylindric cells, ciliary loss and hyperplasia of the secretory cells were observed. Examination of normal sinus mucosa labeled with gold-labeled lectins showed the distribution of sialoglycoconjugates to be mainly in the ciliary layer and the granules in the secretory cells. Inflamed mucosa had increased labeling intensity of gold-labeled WGA in the cilia and the secretory granules. These results indicate that lectin WGA receptors are located in the cilia and secretory granules. Specific changes in the lectin binding pattern were apparent in the inflamed mucosa in the experimentally induced acute sinusitis, in comparison with normal mucosa, conceivably as a part of host defense reactions.

References

  1. Beachey H: Bacterial adherence: Adhesin receptor interactions mediating the attachment of bacteria to mucosal surfaces. Infec Dis 143 (3): 325 345, 1981 https://doi.org/10.1093/infdis/143.3.325
  2. Bouchard P, Moroux Y, Tixier R, Monsigny M: An improved method for purification of wheat germ agglutinin (lectin) by affinity chromatography. Biochimie 58: 1247 1253, 1976 https://doi.org/10.1016/S0300-9084(76)80124-1
  3. Carenfelt C, Lundberg C: Purulent and non purulent maxillary sinus secretions with respect to $pO_2$, $pCO_2$ and pH, Acta Otolaryngol (Stockh) 84: 138 144, 1977 https://doi.org/10.3109/00016487709123952
  4. Cho JH, Kim SJ: Electron Microscopic Demonstration of Sialoglycoconjugates in the Sinus Mucosa of Rabbits after Inoculation of the Influenza A Virus, J Rhinol 5 (1): 33 37, 1998
  5. Damjanov I: Biology of disease, Lectin cytochemistry and histochemistry, Lab Invest 57: 5 19, 1987
  6. Iwata M, Ide H, Terao T et al.: Membrane receptors of mouse lymphocytes for various Iectins. J Biochem 82: 661 669, 1977 https://doi.org/10.1093/oxfordjournals.jbchem.a131741
  7. Johansson P, Kwnlien J, Carlsoo B, Drettner B, Nord CE: Experimental acute sinusitis in rabbits: A bacteriological and histological study, Acta Otolaryngol (Stockh) 105: 357 366, 1988 https://doi.org/10.3109/00016488809097019
  8. Jung YG, Cho JH, Lim HJ: Pathophysiology of Mucociliary Transport. Human Sciences 18 (7): 461 469, 1994
  9. Kaliner M, Shelhamer JH, Borson Bet al.: Human respiratory mucus, Am Rev Respir Dis 134 : 612, 1986
  10. Kim SJ, Hahm SY: Fine structual characterization and localization of lectin receptors in the cultured fibroblast. Korean J Electron Microscopy 31 (1): 49 57, 2001. (Korean)
  11. Mason CM, Azizi SQ, Nogare RD: Respiratory epithelial carbohydrate levels of rats with gram negative bacillary colonization, J Lab Clin Med 120 : 740 745, 1992
  12. Newhouse M, Sanchis J, Bienenstock J et al.: Lung defence mechanism, N Engl J Med 295: 990 998, 1976 https://doi.org/10.1056/NEJM197610282951805
  13. Norlander T, Shimizu T, Larsen P et al.: Early stages in the development of secretory cells in the paranasal sinuses: A multimethodological study in the rabbit, Acta Otolaryngol (Stockh) 118: 114 23, 1998 https://doi.org/10.1080/00016489850155242
  14. Nuutinen J: Activation of impaired rnucociliary function, ActaOtolaryngol99: 605 609, 1985
  15. Otori N, Carlsoo B, Stierana P: Changes in glycoconjugate expression of the sinus mucosa during experimental sinusitis: A lectin histochemical study of the epitheliwn and secretory cell development, Acta Otolaryngol 118: 248 256, 1998 https://doi.org/10.1080/00016489850154982
  16. Reimer A, Huberman D, Klementsson K: The rnucociliary activity of the respiratory tract, Acta Otolaryngol 91 : 139 148,1981 https://doi.org/10.3109/00016488109138492
  17. Reimer A: The effect of carbon dioxide on the activity of cilia, A study on rabbit sinus mucosa in vitro, Acta Otolaryngol (Stockh) 103: 156 60, 1987 https://doi.org/10.3109/00016488709134712
  18. Roseman S: The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesion, Chern Phys Lipids 5 : 270 297, 1970 https://doi.org/10.1016/0009-3084(70)90024-1
  19. Shimizu T, Takahashi Y, Kawaguchi S, Sakakura Y: Hypertrophic and metaplastic changes of secretory cells in rat nasal epitheliwn induced by endotoxin, Am J Respir Crit Care Med 153: 1412 1418, 1996 https://doi.org/10.1164/ajrccm.153.4.8616574
  20. Tanimura H, Morioka H, Murakami Y: Lectin histochemistry in the surface layer of guinea pig middle ear mucosa: the mucous blanket protects the underlying microvilli associated glycocalyx, Immunobiology in Otolaryngology Pregress of a Decade, Oita, Japan, pp. 139 141, 1994
  21. Tos M: Golbet cells and glands in the nose and the paranasal sinuses, In: Proctor D, Andersen I, eds. The nose: upper airway physiology and the atmospheric environment Amsterdam, Elsevier Biomedical Press, pp. 99 144, 1982
  22. Ueno K, Lim DJ: Heterogeneity of glycoconjugates in the secretory cells of the chinchila middle ear and eustachian tubal epithelia: A lectin gold cytochemical study, J Histochem Cytochern 39: 71 80, 1991 https://doi.org/10.1177/39.1.1983875
  23. Wanner A: Clinical aspects of mucociliary transport, Am Rev RespirDis 116:73 125, 1977
  24. Zieske JD, Bernstein IA: Modification of cell surface glycoprotein: addition of fuccsyl residues during epidermal differentiation, J Cell BioI 95 :626 631, 1982 https://doi.org/10.1083/jcb.95.2.626