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Sequence and Genetic Variation of Mitochondrial DNA D-loop Region in Korean Cattle

한우 Mitochondrial DNA D-loop 영역의 염기서열 및 유전변이

  • Chung, E.R. (Division of Applied Animal Sciences, Sangji University) ;
  • Kim, W.T. (Division of Applied Animal Resources, Kangwon National University) ;
  • Kim, Y.S. (Division of Applied Animal Resources, Kangwon National University) ;
  • Lee, J.K. (Division of Applied Animal Resources, Kangwon National University) ;
  • Han, S.K. (College of Animal Husbandry, Konkuk University)
  • 정의룡 (상지대학교 응용동물과학부) ;
  • 김우태 (강원대학교 동물자원학부) ;
  • 김연수 (상원대학교 동물자원학부) ;
  • 이정구 (상원대학교 동물자원학부) ;
  • 한상기 (건국대학교 축산대학)
  • Published : 2002.04.30

Abstract

This study was performed to determine sequences of the mt DNA D-loop region, including $tRNA^{Pro}$ and $tRNA^{Pre}$ and to analysis sequence variation polymorphism in Korean cattle. The resulting sequencies were compared with previously published sequences for other cattle breeds(GenBank J01394). The PCR was used to amplify an 1142bp between nucleotides 15061 and 404 within the D-loop region of mt DNA using specific primers. Korean cattle showed 24 polymorphic sites by nucleotide substitutions and insertions of single base pairs. About 50% of polymorphic sites were found in positions 16042 to 16122 with the most variable region. Among these polymorphic sites, variations at 16055, 16230 and 16260 bp were detected as new sequence variants in Korean cattle. These specific polymorphic sites have not been reported in the Japanese black cattle and European cattle. Therefore, mt DNA variants in the D-loop region may be used as genetic markers for specifying Korean cattle. The frequencies of positions 169, 16302, 16093, 16042, 16119 with a high level of sequence polymorphism were 0.81, 0.56, 0.56, 0.50 and 0.43, respectively. In comparison of genetic distances, Korean cattle showed the more closely to European cattle as Bos taurus than Bos indicus such as African and India breeds. In conclusion, these mt DNA sequence polymorphisms in the D-loop region for Korean cattle may be useful for the analysis of cytoplasmic genetic variation and associations with economic important traits and genetic analysis of maternal lineage.

Keywords

mt DNA D-loop region;Sequence variation;Korean cattle

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