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Studies on the MC1R Gene Frequencies in Landrace, Large White, Duroc and Jeju Native Black Pigs

랜드레이스, 대요크셔, 듀록 및 제주 흑돈의 Melanocortin 1 Receptor(MC1R) 유전자의 유전자형 분석

  • Cho, I.C. (National Jeju Agricultural Experiment Station, R.D.A) ;
  • Lee, J.G. (Division of Animal Science, Gyeongsang National University) ;
  • Jung, J.G. (National Jeju Agricultural Experiment Station, R.D.A) ;
  • Yang, B.S. (National Jeju Agricultural Experiment Station, R.D.A) ;
  • Kang, S.Y. (National Jeju Agricultural Experiment Station, R.D.A) ;
  • Kim, B.W. (Division of Animal Science, Gyeongsang National University)
  • 조인철 (농촌진흥청 제주농업시험장) ;
  • 이정규 (경상대학교 축산과학부) ;
  • 정진관 (농촌진흥청 제주농업시험장) ;
  • 양보석 (농촌진흥청 제주농업시험장) ;
  • 강승률 (농촌진흥청 제주농업시험장) ;
  • 김병우 (경상대학교 축산과학부)
  • Published : 2002.04.30

Abstract

This study was conducted to investigate the genotypes and frequencies of Melanocortin 1 Receptor(MC1R) genes in pigs which plays a central role in regulation of eumelanin (black/brown) and phaeomelanin(red/yellow) pigment synthesis within the mammalian melanocytes. Four different breeds of pigs(20 Landrace, 20 Yorkshire, 20 Duroc, and 93 Jeju native black pigs) were used and PCR-RFLP analysis of MC1R gene was also carried out. Two regions of MC1R genes (428bp and 405bp) were amplified using two specific primers (MERL1-EPIG2, EPIG1-EPIG3), respectively and MC1R allele were determined using 2 restriction enzymes (BspHⅠ, AccⅡ). The results of this experiment indicated that MC1R allelic type in Landrace, Large Yorkshire and Duroc were MC1R *2 (Ep), MC1R *2 (Ep), MC1R *4 (e), respectively. However, various allelic types of MC1R genes were detected in Jeju native black pigs. MC1R allelic type of Jeju black pigs was MC1R*2 type as in Meishan and Large black breeds or MC1R*3 type as in Hampshire and Berkshire breeds and the gene frequencies of ED1 and ED2 were 0.554 and 0.446 in average.

Keywords

MC1R gene;PCR-RFLP;Jeju native black pigs

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