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Polymorphisms of the Exons 13, 15 and 16 of Transferrin Gene in Cheju Horses

제주마 Transferrin Gene Exon 13, 15 및 16의 다형현상

  • Kim, N.Y. (Department of Animal Biotechnology, Cheju National University) ;
  • Lee, S.S. (National Jeju Agricultural Experiment Station, R.D.A.) ;
  • Yang, Y.H. (Department of Animal Biotechnology, Cheju National University)
  • 김남영 (제주대학교 동물자원과학과) ;
  • 이성수 (농촌진흥청 제주농업시험장) ;
  • 양영훈 (제주대학교 동물자원과학과)
  • Published : 2002.08.31

Abstract

This study was conducted to determine the polymorphism of transferrin exons 13, 15 and 16 by Single-Strand Conformation Polymorphism(SSCP) analysis and to compare their genotypes of Cheju horse Group I (Cheju Institute), Cheju horse Group II (farms), and Thoroughbred (KRA). SSCP of transferrin exon 13, 15, and 16 showed two (A, B), three (A, B, C) and three (A, B, C) codominant alleles, respectively. The Group I and Thoroughbred showed the similar frequencies of allele A and B in transferrin exon 13, but only allele A was observed in Group Ⅱ. In transferrin exons 15 and 16, the frequencies of each allele were different in each Groups. The multiple allele frequencies in exons 15 and 16 suggested that the genotyping of this locus could be used to identify an individual and to test the parentage of offspring. The probability for parentage exclusion were 0.46 and 0.374 for exons 15 and 16 for Cheju horse Group I. Among the 13 combined genotypes of exons 13, 15 and 16, the genotype AA-AB-AB (0.372) is the most common in Cheju horse Group I, but genotype AA-AA-AA is common in the Cheju horse Group II (0.366) and Thoroughbred (0.767). The present study showed two new SNP, which was at the cDNA position 1626 (A/G) in B allele of the exon 13 and 2075 (C/T) in C allele of the exon 16 resulting in amino acid change (Threonine $\longrightarrow$ Methionine). Result showed that polymorphism of exons 13, 15 and 16 in Cheju horses was as high as in Thoroughbred and there was a differences of transferrin allele frequencies in Cheju horses.

Keywords

SSCP;Transferrin;SNP;Cheju Horse

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