Antihypertensive Effects of Casein Hydrolysate in Spontaneously Hypertensive Rats

자연발증고혈압쥐에서 카제인 가수분해물의 혈압강하효과

  • Kim, H.S. (National Livestock Research Institute, R.D.A.) ;
  • In, Y.M. (National Livestock Research Institute, R.D.A.) ;
  • Jeong, S.G. (National Livestock Research Institute, R.D.A.) ;
  • Ham, J.S. (National Livestock Research Institute, R.D.A.)
  • 김현수 (농촌진흥청 축산기술연구소) ;
  • 인영민 (농촌진흥청 축산기술연구소) ;
  • 정석근 (농촌진흥청 축산기술연구소) ;
  • 함준상 (농촌진흥청 축산기술연구소)
  • Published : 2002.08.31


The aim of this study was to determine if a low-molecular weight casein hydrolysate has an anti- hypertensive effect in spontaneously hypertensive rats (SHR). Prior to the in vivo experiment, the casein hydrolysate was confirmed to be resistant to gastrointestinal digestion by confirming the retention of its potency as an inhibitor of angiotensin I-concerting enzyme after incubation with pepsin, trypsin, or chymotrypsin. The in vivo anti-hypertensive effect of the hydrolysate was determined by the tail cuff method. Following an oral administration of the hydrolysate solution, the systolic blood pressure (SBP) decreased by 12.9% (-28.9mmHg; P<0.05) at 3 h after the administration at a dose of 500mg/kg body weight. When the hydrolysate was administered as an emulsion with 30% egg yolk, its anti-hypertensive effect was even more greater at the same dose(-30.8mmHg or -15.9%; P<0.01). In a 50-day long-term trial where the casein hydrolysate was administered once a day, the SBP-lowering effect of the hydrolysate was apparent (P<0.05) from day 35 through the end. Moreover, organ weights and plasma glutamate oxaloacetate transaminase and glutamate pyruvate transaminase activities of the administered SHR were not significantly different from those of controls at the end of the long-term trial.




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